DeNovo NT Allograft

Tompkins, Marc; Adkisson, H. Davis; Bonner, Kevin F.

doi:10.1053/j.otsm.2013.03.005

Cited by 40 publications

(33 citation statements)

References 40 publications

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“…Fibrin glue, popular because of its ease of use and suitability for arthroscopy, is used for BioCart ™ II, MACI, RevaFlex ™ , and CaReS ® . Since chondrocytes proliferate and migrate within fibrin gels (262, 263), integration is not hindered. It is worth noting that fixation methods are often designed concomitantly with products.…”

Section: Tissue Engineering Strategies Used In Current Clinical Prmentioning

confidence: 99%

Cell-based tissue engineering strategies used in the clinical repair of articular cartilage

2016

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One of the most important issues facing cartilage tissue engineering is the inability to move technologies into the clinic. Despite the multitude of review articles on the paradigm of biomaterials, signals, and cells, it is reported that 90% of new drugs that advance past animal studies fail clinical trials (1). The intent of this review is to provide readers with an understanding of the scientific details of tissue engineered cartilage products that have demonstrated a certain level of efficacy in humans, so that newer technologies may be developed upon this foundation. Compared to existing treatments, such as microfracture or autologous chondrocyte implantation, a tissue engineered product can potentially provide more consistent clinical results in forming hyaline repair tissue and in filling the entirety of the defect. The various tissue engineering strategies (e.g., cell expansion, scaffold material, media formulations, biomimetic stimuli, etc.) used in forming these products, as collected from published literature, company websites, and relevant patents, are critically discussed. The authors note that many details about these products remain proprietary, not all information is made public, and that advancements to the products are continuously made. Nevertheless, by fully understanding the design and production processes of these emerging technologies, one can gain tremendous insight into how to best use them and also how to design the next generation of tissue engineered cartilage products.

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Section: Tissue Engineering Strategies Used In Current Clinical Prmentioning

confidence: 99%

Cell-based tissue engineering strategies used in the clinical repair of articular cartilage

2016

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“…Our results highlight the importance of adequate cell seeding, a parameter also considered in the technique of cell‐seeded collagen matrix‐supported ACI (Steinwachs ) and in high‐density chondrospheres (Schubert et al , ). ECP isolation from fetal cartilage yields highly viable cells (Tompkins et al , ), which maintain their strong chondrogenic potential after 2D expansion, an advantage over the use of ACs, which dedifferentiate during 2D tissue culture.…”

Section: Discussionmentioning

confidence: 99%

Human chondroprogenitors in alginate-collagen hybrid scaffolds produce stable cartilagein vivo

Studer

Cavalli

Formica

et al. 2016

J Tissue Eng Regen Med

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The goal of this study was to evaluate human epiphyseal chondroprogenitor cells (ECPs) as a potential new cell source for cartilage regeneration. ECPs were compared to human bone marrow stromal cells (MSCs) and human adult articular chondrocytes (ACs) for their chondrogenic potential and phenotypic stability in vitro and in vivo. The cells were seeded in Optimaix-3D scaffolds at 5 × 10 cells/mm and gene expression, matrix production and mechanical properties were analysed up to 6 weeks. In vitro, ECPs synthesized consistently high collagen 2 and low collagen 10. AC-seeded constructs exhibited high donor variability in GAG/DNA values as well as in collagen 2 staining, but showed low collagen 10 production. MSCs, on the other hand, expressed high levels of collagen 2 but also of collagens 1 and 10, and were therefore not considered further. In vivo, there was considerable loss of matrix proteins in ECPs compared to in vitro cultured samples. To overcome this, a second implantation study investigated the effect of mixing cells with alginate prior to seeding in the scaffold. ECPs in alginate maintained their cartilage matrix and resisted mineralization and vessel infiltration better 6 weeks after subcutaneous implantation, whereas ACs lost their chondrogenic matrix completely. This study shows the great potential of ECPs as an off-the-shelf, highly chondrogenic cell type that produces stable cartilage in vivo. Copyright © 2016 John Wiley & Sons, Ltd.

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“…When implanting DeNovo NT, the debridement of the fibrous and calcified tissue of the defect should be performed without violating the subchondral bone layer. Then the implant is added to the lesion site and using fibrin glue will help the tissue to be maintained in place [64,65]. Recently, this juvenile particulate cartilage has been employed in patellar lesions with success.…”

Section: Commercially Available Products For Osteochondral Regenerationmentioning

confidence: 99%

Commercial Products for Osteochondral Tissue Repair and Regeneration

Bicho

Pina

Reis

et al. 2018

Osteochondral Tissue Engineering

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The osteochondral tissue represents a complex structure composed of four interconnected structures, namely hyaline cartilage, a thin layer of calcified cartilage, subchondral bone, and cancellous bone. Due to the several difficulties associated with its repair and regeneration, researchers have developed several studies aiming to restore the native tissue, some of which had led to tissue-engineered commercial products. In this sense, this chapter discusses the good manufacturing practices, regulatory medical conditions and challenges on clinical translations that should be fulfilled regarding the safety and efficacy of the new commercialized products. Furthermore, we review the current osteochondral products that are currently being marketed and applied in the clinical setting, emphasizing the advantages and difficulties of each one.

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DeNovo NT Allograft

Cited by 40 publications

References 40 publications

Cell-based tissue engineering strategies used in the clinical repair of articular cartilage

Cell-based tissue engineering strategies used in the clinical repair of articular cartilage

Human chondroprogenitors in alginate-collagen hybrid scaffolds produce stable cartilagein vivo

Commercial Products for Osteochondral Tissue Repair and Regeneration

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