Denitrification capabilities of two biological phosphorus removal sludges dominated by different ‘<i>Candidatus</i> Accumulibacter’ clades

Flowers, Jason J.; He, Shaomei; Yılmaz, Şafak; Noguera, Daniel R.; McMahon, Katherine D.

doi:10.1111/j.1758-2229.2009.00090.x

Cited by 195 publications

(163 citation statements)

References 22 publications

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“…Interestingly, those encoding the nitrate reductases responsible for reducing nitrate to ammonia (nirB and nirD) were detected in the genome of T. japonica (Table 4), suggesting that this organism can reduce nitrate by dissimilatory nitrate reduction to ammonia. Thus, Tetrasphaera may be able to couple nitrate and/or nitrite reduction to phosphorus uptake, which is similar to the behavior described previously for Accumulibacter clade IA members (Flowers et al, 2009).…”

Section: Metabolic Reconstructionsupporting

confidence: 58%

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Kristiansen

Nguyen²,

Saunders³

et al. 2012

The ISME Journal

191

154

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Members of the genus Tetrasphaera are considered to be putative polyphosphate accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) from wastewater. Although abundant in Danish full-scale wastewater EBPR plants, how similar their ecophysiology is to 'Candidatus Accumulibacter phosphatis' is unclear, although they may occupy different ecological niches in EBPR communities. The genomes of four Tetrasphaera isolates (T. australiensis, T. japonica, T. elongata and T. jenkinsii) were sequenced and annotated, and the data used to construct metabolic models. These models incorporate central aspects of carbon and phosphorus metabolism critical to understanding their behavior under the alternating anaerobic/aerobic conditions encountered in EBPR systems. Key features of these metabolic pathways were investigated in pure cultures, although poor growth limited their analyses to T. japonica and T. elongata. Based on the models, we propose that under anaerobic conditions the Tetrasphaerarelated PAOs take up glucose and ferment this to succinate and other components. They also synthesize glycogen as a storage polymer, using energy generated from the degradation of stored polyphosphate and substrate fermentation. During the aerobic phase, the stored glycogen is catabolized to provide energy for growth and to replenish the intracellular polyphosphate reserves needed for subsequent anaerobic metabolism. They are also able to denitrify. This physiology is markedly different to that displayed by 'Candidatus Accumulibacter phosphatis', and reveals Tetrasphaera populations to be unusual and physiologically versatile PAOs carrying out denitrification, fermentation and polyphosphate accumulation.

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Section: Metabolic Reconstructionsupporting

confidence: 58%

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Kristiansen

Nguyen²,

Saunders³

et al. 2012

The ISME Journal

191

154

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“…PAOII can only use nitrite but not nitrate as electron acceptors [11]. In F-SBR and G-SBR, the ratios of PAOII to PAOs were 72 % (SE mean =1.4 %) and 76 % (SE mean =0.9 %), respectively.…”

Section: 1 Fish In the Two Sbrsmentioning

confidence: 95%

“…In addition, Cy5-labeled Acc-I-444 was used to target PAOI, and FAM-labeled Acc-II-444 was used to target PAOIIA, IIC, and IID Accumulibacter, respectively [11]. The standard error of the mean value (SE mean ) was calculated as the standard deviation divided by the square root of the number of images.…”

Section: Microbial Community Analysis Of the Floc And Granular Sludgesmentioning

confidence: 99%

Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors

Wang

Jiang

Hong

et al. 2015

Chemical Engineering Journal

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Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors, Chemical Engineering Journal (2014), doi: http://dx.doi.org/10.1016/j.cej. 2014.09.065 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACT:Granule formation frequently occurs in denitrifying phosphorus (P) removal systems.The differences between granules and flocculants, including P removal capacities, bio-physiochemical properties, and microorganism distribution and diversity, are however still poorly understood. Physical and biochemical characteristics of granules and sludge flocs were investigated through two laboratory-scale sequencing batch reactors (SBRs). One reactor was operated as a flocculent SBR and the other 2 was operated as a granular SBR, using a granule diameter of 1.92 ± 0.78 mm. Granular sludge had a higher tolerance to inhibitors (nitrite/free nitrite acids (FNAs)), and a higher percentage of P accumulating organisms (PAOs) (72 % vs. 64 % in flocs) than flocculent sludge. The granule crush tests and higher PAOII (unable to use nitrate as an electron acceptor) to PAOs ratios (over 72 %) by fluorescent in situ hybridization showed that in both reactors, glycogen accumulating organisms (GAOs) were mainly responsible for nitrate to nitrite reduction, and PAOII further reduced nitrite to nitrogen gas in association with anoxic P uptake; GAOs wash-out weakened the mutual relationship between GAOs and PAOII to some extent, which made denitrification of nitrate to nitrite inefficient and weakened subsequent anoxic P removal. GAOs existed mainly on the surface of the granules, whereas PAOs (PAOI+PAOII ) were distributed both on the surface and in the interior of the granules. Thus, GAOs had easier access to carbon sources but were at risk of suffering from exposure to FNA.

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“…Numerous studies by Flowers et al [52], He et al [15,53] and Oehmen et al [30,31,33] have found that lower COD/P ratios of 10-15 should be used for successful enrichment (>80% of all bacteria) of Accumulibacter in lab-scale reactors. However, there are some contradicting studies which indicate that low C/P ratios are not sufficient for outcompeting GAOs nor achieving low phosphorus effluent even in lab-scale optimal conditions.…”

Section: C/p Ratio In Influentmentioning

confidence: 99%

From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor

et al. 2013

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A laboratory scale sequencing batch reactor (SBR), fed with synthetic wastewater containing a mixture of organic compounds, was operated for nearly six months. Despite maintaining the same operational conditions, a deterioration of enhanced biological phosphorus removal (EBPR) occurred after 40 days of SBR operation. The Prel/Cupt ratio decreased from 0.28 to 0.06 P-mol C-mol−1, and C requirements increased from 11 to 32 mg C h−1 g−1 of mixed liquor suspended solids. A FISH analysis showed that the percentage of Accumulibacter in an overall community of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) dropped from 93% to 13%. An increase in abundance of Gammaproteobacteria (from 2.6% to 22%) and Alphaproteobacteria (from 1.8% to 10%) was observed. The number of Competibacter increased from 0.5% to nearly 9%. Clusters 1 and 2 of Defluviicoccus-related GAOs, not detected before deterioration, constituted 35% and 27% of Alphaproteobacteria, respectively. We concluded that lab-scale experiments should not be extended implicitly to full-scale EBPR systems because some bacterial groups are detected mainlyin lab-scale reactors. Well-defined, lab-scale operational conditions reduce the number of ecological niches available to bacteria.

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Denitrification capabilities of two biological phosphorus removal sludges dominated by different ‘Candidatus Accumulibacter’ clades

Cited by 195 publications

References 22 publications

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors

From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor

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