Dengue nonstructural protein 1 antigen in the urine as a rapid and convenient diagnostic test during the febrile stage in patients with dengue infection

Chuansumrit, Ampaiwan; Chaiyaratana, Wathanee; Tangnararatchakit, Kanchana; Yoksan, Sutee; Flamand, Marie; Sakuntabhai, Anavaj

doi:10.1016/j.diagmicrobio.2011.08.020

Cited by 35 publications

(26 citation statements)

References 6 publications

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“…In a previous study, the NS1 antigen was detected in urine samples collected on days 2-10 after disease onset, positive rates of NS1 antigen using NS1 antigen ELISA were 68.4z and 63.9z in 19 DF and 36 DHF samples, respectively (21). However, the utility of NS1 antigen ELISA was not determined at each disease phase.…”

Section: Discussionmentioning

confidence: 91%

“…Previous studies by other investigators have shown the presence of viral genome in the urine samples of dengue patients (12,20). Additionally, NS1 antigen has been detected in urine samples from days 2-10 after the onset of the disease (21). However, determining the applicability of NS1 ELISA at each phase of the disease would confirm the usefulness of NS1 antigen detection in urine samples for DENV diagnosis.…”

Section: Introductionmentioning

confidence: 87%

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Detecting Dengue Virus Nonstructural Protein 1 (NS1) in Urine Samples Using ELISA for the Diagnosis of Dengue Virus Infection

et al. 2015

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SUMMARY:Dengue virus (DENV) infection is a serious global health threat. For the surveillance and control of dengue, there is a need for robust diagnostic tools that are relatively easy to use and reliable in various clinical settings. We investigated the applicability of NS1 antigen detection in urine samples for the diagnosis of DENV. About 118 urine samples, obtained from 96 dengue patients at various phases of disease, were used for this study. NS1 antigen was detected by ELISA in the urine samples obtained from patients after 2-17 days of disease onset. Positive detection rates of NS1 antigen ranged between 13-43z. Based on real-time RT-PCR, positive detection rates of viral genome in the urine samples ranged between 20-33z on days 0 to 15. On days 11 to 15 after the disease onset, NS1 antigen was detected at similar rates in serum and urine samples. Additionally, NS1 antigen was detected in 2 urine samples, but not in the serum samples, on days 7 and 16 after the onset of the disease. The results confirm the applicability of NS1 antigen detection in urine samples using ELISA to diagnose acute DENV infection and suggests that the assay is potentially useful when only limited amounts of serum samples are available and in limited resource settings.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 87%

Detecting Dengue Virus Nonstructural Protein 1 (NS1) in Urine Samples Using ELISA for the Diagnosis of Dengue Virus Infection

et al. 2015

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“…These patient samples were collected from 17 countries in Latin America, Asia and Oceania, and the most studies were conducted in Brazil (27%) [29], [34], [37], [41], [45], [47], [52], [54], Vietnam (13%) [32], [33], [36], [49], Malaysia (13%) [26], [36], [42], [46] and Thailand (13%) [31], [36], [43], [48]. Regarding the design of the studies, they were classified into two types: prospective and retrospective cohorts, of which only twelve reported that their samples were collected during dengue outbreaks [25], [26], [30], [37], [39], [40], [44], [45], [51]–[54].…”

Section: Resultsmentioning

confidence: 99%

A Meta-Analysis of the Diagnostic Accuracy of Two Commercial NS1 Antigen ELISA Tests for Early Dengue Virus Detection

2014

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BackgroundDengue virus (DENV) NS1 antigen detection is regarded as an early diagnostic marker. Accordingly, several studies have evaluated the performance of tests that utilize NS1 capture, but the results of individual studies may be limited due to the restricted sample size of the patients recruited. Therefore, our objective was to perform a meta-analysis of the diagnostic accuracy of two commercial NS1 ELISAs (Panbio and Platelia).Methods and ResultsStudies of interest were found in PubMed, Embase and Google Scholar databases using defined inclusion/exclusion criteria. A total of 30 studies containing 12,105 total enrolled patients were included. The results were as follows: 1) Panbio assays showed low overall performance, sensitivity 66% (95% confidence interval (CI) 61–71), specificity 99% (95% CI 96–100), positive likelihood ratio (LR+) 98 (95% CI 20–464), negative likelihood ratio (LR-) 0.3 (95% CI 0.2–0.4), diagnostic odds ratio (DOR) 289 (95% CI 59–1412); 2) Platelia assays showed high overall performance, sensitivity 74% (95% CI 63–82), specificity 99% (95% CI 97–100), LR+ 175 (95% CI 28–1099), LR- 0.3 (95% CI 0.2–0.4), DOR 663 (95% CI 98–4478). The lowest sensitivity values were for secondary infections (57% [95% CI 47–67] and 66% [95% CI 53–77] for Panbio and Platelia, respectively) and for the detection of DENV4. Regarding clinical manifestations, the sensitivity of Platelia was 69% (95% CI 43–86) and 60% (95% CI 48–70) for fever and dengue hemorrhagic fever, respectively. In addition, the sensitivity of both tests was slightly lower for samples from Southeast Asia and Oceania.ConclusionDENV1 samples gave higher sensitivity results for both tests. We observed that factors negatively influencing the tests, such as the type of infection, geographical origins of samples and viral serotypes, require further investigation to optimize the diagnostic accuracy.

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“…Diagnosis of other viral infections in urine and saliva samples has been reported for human immunodeficiency virus (HIV), Hepatitis A, B, and C, and rubella [14][15][16][17][18][19][20], and more recently in other flaviviruses, West-Nile virus (WNV) [21][22][23] and Dengue (DENV) serotypes -1, -2, -3, and -4 [24][25][26][27][28][29][30][31][32][33][34][35]. WNV can be detected longer in urine than serum [21] and can be isolated from urine [22,23].…”

Section: Advantages Of Non-invasive Sample Collectionsmentioning

confidence: 99%

Advantage of urine based molecular diagnosis of Zika virus

Lamb

Bartolone²,

Kutluay

et al. 2016

Int Urol Nephrol

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Dengue nonstructural protein 1 antigen in the urine as a rapid and convenient diagnostic test during the febrile stage in patients with dengue infection

Cited by 35 publications

References 6 publications

Detecting Dengue Virus Nonstructural Protein 1 (NS1) in Urine Samples Using ELISA for the Diagnosis of Dengue Virus Infection

Detecting Dengue Virus Nonstructural Protein 1 (NS1) in Urine Samples Using ELISA for the Diagnosis of Dengue Virus Infection

A Meta-Analysis of the Diagnostic Accuracy of Two Commercial NS1 Antigen ELISA Tests for Early Dengue Virus Detection

Advantage of urine based molecular diagnosis of Zika virus

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