Dengue-2 vaccine: preparation from a small-plaque virus clone

Eckels, Kenneth H.; Harrison, V. R.; Summers, Peter L.; Russell, Philip K.

doi:10.1128/iai.27.1.175-180.1980

Cited by 56 publications

(13 citation statements)

References 16 publications

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“…Genetic changes responsible for flavivirus attenuation are not well understood at the molecular level although passage of the viruses in cell cultures may result in loss of virulence (14,25,65,73). On the other hand, in vivo passage level (in vivo transmission) of the viruses in its vertebrate host (human) and invertebrate host/vector (mosquito/tick) might have some influence on the virulence factor.…”

Section: Discussionmentioning

confidence: 99%

Detection of the Disease Severity‐Related Molecular Differences among New Thai Dengue‐2 Isolates in 1993, Based on Their Structural Proteins and Major Non‐Structural Protein NS1 Sequences

Thant

Morita

Igarashi

1996

Microbiology and Immunology

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We determined the nucleotide sequences of the whole structural protein gene of four new dengue-2 viruses by the primer extension dideoxy chain termination method, using multiple cDNA clones for six overlapping gene regions. The nucleotide sequences of the major non-structural protein NS1 gene of these viruses were also determined by direct sequencing of the reverse-transcription polymerase chain reaction products. These viruses were isolated from dengue patients with different clinical severities in Nakhon Phanom, Northeastern Thailand in 1993. The results were compared with the sequences of prototype New Guinea C strain and other reference strains. All four viruses revealed highest homology to New Guinea C strain. The homology between each of the four strains and New Guinea C strain varies from 95.09% to 95.29% in its nucleotide sequences, and from 97.24% to 97.78% in its amino acid sequences covering all structural proteins and NS1 protein. The PreM region shows the highest divergence (6.59% to 7.32%) in its nucleotide sequence, whereas C protein is most highly conserved (only 1.75% to 2.63% divergence). Our data showed that there are certain molecular differences in the genomic structure of these four new isolates, which indicate the possibility that these changes are related with the virulence of the virus strains.

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Section: Discussionmentioning

confidence: 99%

Detection of the Disease Severity‐Related Molecular Differences among New Thai Dengue‐2 Isolates in 1993, Based on Their Structural Proteins and Major Non‐Structural Protein NS1 Sequences

Thant

Morita

Igarashi

1996

Microbiology and Immunology

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“…A new attenuated strain, 181/clone 25 (181/25), was derived by 18 plaque-to-plaque passages of the parent product (28). Strain 181/25 had all the hallmarks of attenuation (29,30), including a small-plaque phenotype, temperature sensitivity, decreased virulence (in mice), and low levels of viremia (in monkeys). When tested in mouse or monkey challenge models, this vaccine was protective and demonstrated reduced virulence compared to that of its nonattenuated parent strain.…”

Section: Figmentioning

confidence: 99%

Vaccine and Therapeutic Options To Control Chikungunya Virus

2018

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Beginning in 2004, chikungunya virus (CHIKV) went from an endemic pathogen limited to Africa and Asia that caused periodic outbreaks to a global pathogen. Given that outbreaks caused by CHIKV have continued and expanded, serious consideration must be given to identifying potential options for vaccines and therapeutics. Currently, there are no licensed products in this realm, and control relies completely on the use of personal protective measures and integrated vector control, which are only minimally effective. Therefore, it is prudent to urgently examine further possibilities for control. Vaccines have been shown to be highly effective against vector-borne diseases. However, as CHIKV is known to rapidly spread and generate high attack rates, therapeutics would also be highly valuable. Several candidates are currently being developed; this review describes the multiple options under consideration for future development and assesses their relative advantages and disadvantages.

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“…After World War II, new endemic patterns of disease, accompanied by an increased incidence of complicated dengue (DHF and DSS), emerged in southeast Asia (96). [67][68][69][70][71][72] were the first to describe epidemic DHF in the Philippine Islands in 1954. Halstead (95) has suggested that DHF was not a new disease and that dengue epidemics in which patients had symptoms of severe hemorrhage and shock were described in Queensland (1897), the southern United States (1922), Durban, South Africa (1927), Greece (1928), and Formosa (1931).…”

Section: Epidemic Patternsmentioning

confidence: 99%