Dengue 2 genotypes in the state of Oaxaca, Mexico

Cisneros, Alejandro; Diaz-Badillo, Alvaro; Cruz‐Martínez, Gibrán; Tovar, Rosalinda; Ramírez-Palacios, Luis Román; Jiménez-Rojas, F.; Beaty, Barry J.; Black, William C.; Muñoz, Marı́a de Lourdes

doi:10.1007/s00705-005-0595-9

Cited by 15 publications

(18 citation statements)

References 33 publications

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“…These results confirmed the circulation of the four serotypes in the states of Oaxaca and Veracruz, Mexico (36). This study also showed that DENV-2 is present in serum at a higher frequency, consistent with previous assessments of the prevalence of these serotypes in the epidemics of 2000–2001 [37] and 2005–2006 [20,21]. …”

Section: Resultssupporting

confidence: 92%

“…Fluorescence variability was very low in our assays, suggesting this technology will be very useful for DENV diagnoses. Detection of two cases of dual infections (DENV-2 and -1; and DENV-2 and -3) confirm the feasibility of using this microarray assay to diagnose dual infections (Figure 4) [20,21,44]. …”

Section: Resultsmentioning

confidence: 65%

“…Reverse transcription-polymerase chain reaction (RT-PCR) is another potential method for detection of viral RNA [15–19]. Initial studies demonstrated the need to obtain virus isolates from C6/36 cells infected with each virus sample before detection by RT-PCR [20,21]. This method is also limited by the number of species that can be detected and identified in a single test, often requiring multiple parallel reactions [22].…”

Section: Introductionmentioning

confidence: 99%

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A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes

Diaz-Badillo

Muñoz

Pérez-Ramírez

et al. 2014

Sensors

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Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

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A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes

Diaz-Badillo

Muñoz

Pérez-Ramírez

et al. 2014

Sensors

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“…DENV-2 strains of the American/Asian genotype circulating in Oaxaca, Mexico, are probably from South-East Asia [70]. The 2001 isolates of American/Asian DENV-2 were most similar to the Jamaican and Venezuelan isolates MARA3, LARD1996 and LARD1910.…”

Section: Resultsmentioning

confidence: 99%

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

et al. 2017

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Dengue, the predominant arthropod-borne viral disease affecting humans, is caused by one of four distinct serotypes (DENV-1, -2, -3 or -4). A literature analysis and review was undertaken to describe the molecular epidemiological trends in dengue disease and the knowledge generated in specific molecular topics in Latin America, including the Caribbean islands, from 2000 to 2013 in the context of regional trends in order to identify gaps in molecular epidemiological knowledge and future research needs. Searches of literature published between 1 January 2000 and 30 November 2013 were conducted using specific search strategies for each electronic database that was reviewed. A total of 396 relevant citations were identified, 57 of which fulfilled the inclusion criteria. All four dengue virus serotypes were present and co-circulated in many countries over the review period (with the predominance of individual serotypes varying by country and year). The number of countries in which more than one serotype circulated steadily increased during the period under review. Molecular epidemiology data were found for Argentina, Bolivia, Brazil, the Caribbean region, Colombia, Ecuador, Mexico and Central America, Paraguay, Peru and Venezuela. Distinct lineages with different dynamics were found in each country, with co-existence, extinction and replacement of lineages occurring over the review period. Despite some gaps in the literature limiting the possibility for comparison, our review has described the molecular epidemiological trends of dengue infection. However, several gaps in molecular epidemiological information across Latin America and the Caribbean were identified that provide avenues for future research; in particular, sequence determination of the dengue virus genome is important for more precise phylogenetic classification and correlation with clinical outcome and disease severity.

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“…7,8,10 Several factors could be involved in this increase in the number of DEN cases in Oaxaca, such as the viral genotype, vector competence, and the socioeconomic level of the population. The Asian-American genotype of DEN2 was detected in the year 2000 20,21 in the state and this was the main causative agent of the outbreak in the state of Yucatan in 2002. 22 The increase in the number of DEN cases in Oaxaca could be related to the circulation of this relatively new genotype although DEN1 and DEN4 were the most frequently found serotypes in our serological study.…”

Section: Discussionmentioning

confidence: 99%