Dendrobium officinale polysaccharides alleviate colon tumorigenesis via restoring intestinal barrier function and enhancing anti-tumor immune response

Liang, Jian; Li, Hailun; Chen, Jianqiang; He, Lian; Du, Xiongming; Zhou, Lian; Xiong, Qingping; Lai, Xiaoping; Yang, Yiqi; Huang, Song; Hou, Shaozhen

doi:10.1016/j.phrs.2019.104417

Cited by 96 publications

(58 citation statements)

References 52 publications

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“…Dendrobium is a genus of valuable medicinal herbs in the family Orchidacea [1], containing 74 species and two varieties in China [2] of which many species can be used as medicine [3,4]. Dendrobium officinale is a precious species and famously known as one of the nine immortal herbs [5], as it possesses many kinds of physiological functions [6], including immune modulation [7], antitumor activity [8], and helping to reduce levels of blood glucose [9,10]. However, wild D. officinale is scarce because it requires a peculiar growth environment and takes a long time to grow.…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates

Zuo

Zhang

et al. 2020

Metabolites

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Dendrobium officinale, a precious herbal medicine, has been used for a long time in Chinese history. The metabolites of D. officinale, regarded as its effective components to fight diseases, are significantly affected by cultivation substrates. In this study, ultra-performance liquid chromatography mass spectrometry (UPLC-MS/MS) was conducted to analyze D. officinale stems cultured in three different substrates: pine bark (PB), coconut coir (CC), and a pine bark: coconut coir 1:1 mix (PC). A total of 529 metabolites were identified. Multivariate statistical analysis methods were employed to analyze the difference in the content of metabolites extracted from different groups. By the criteria of variable importance in projection (VIP) value ≥1 and absolute log2 (fold change) ≥1, there were a total of 68, 51, and 57 metabolites, with significant differences in content across groups being filtrated out between PB and PC, PB and CC, and PC and CC, respectively. The comparisons among the three groups revealed that flavonoids were the metabolites that fluctuated most. The results suggested the D. officinale stems from the PB group possessed a higher flavonoid content. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that the significantly regulated metabolites were mainly connected with flavonoid biosynthesis. A comprehensive profile of the metabolic differentiation of D. officinale planted in different substrates was provided, which supports the selection of an optimum cultivation substrate for a higher biomass yield of D. officinale.

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Section: Introductionmentioning

confidence: 99%

Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates

Zuo

Zhang

et al. 2020

Metabolites

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“…When the tight junction is closed, it can prevent the passage of bacteria or toxic macromolecules. Studies have shown that polysaccharides of traditional Chinese medicine have an important role in enhancing intestinal immunity, such as Ganoderma atrum polysaccharide, Codonopsis pilosula polysaccharide, and Dendrobium officinale polysaccharides have significant effects on repairing intestinal mucosal structure and enhancing intestinal mucosal immunity [ 10 – 12 ]. Atractylenolide I and PAMK have been shown to stimulate the migration and proliferation of intestinal epithelial cells and have a significant effect on repairing intestinal mucosal epithelium [ 13 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

PAMK Relieves LPS-Induced Enteritis and Improves Intestinal Flora Disorder in Goslings

Xiang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Polysaccharide of Atractylodes macrocephala Koidz (PAMK) is a biologically active component of Atractylodes macrocephala, which has the effect of maintaining the immune homeostasis of the body. Therefore, this study constructed a model of PAMK to relieve LPS-induced gosling enteritis and observed the morphological changes of the small intestine after HE staining. ELISA was used to detect serum CRP, IL-1β, IL-6, and TNF-α levels; immunohistochemistry was used to detect the positive rate of IgA in the small intestine; TLR4, occludin, ZO-1, cytokines, and immunoglobulin mRNA expression in the small intestine were detected by qPCR; and intestinal flora of gosling excrement was analyzed by 16S rDNA sequencing to analyze the protective effect of PAMK on goslings enteritis and the impact on intestinal flora. The results showed that PAMK relieves LPS-induced gosling enteritis by maintaining the small intestine morphology, cytokine, tight junctions, and immunoglobulin relatively stable and improving the disorder of intestinal flora.

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“…At present, studies of D. candidum have mainly focused on its functional polysaccharide, which is related to antitumor, antiobesity and blood sugar-reducing activities ( 31 , 32 ). However, the antioxidant, anti-diabetes, and anti-inflammatory effects of Dendrobium polyphenols have received limited attention ( 33 ).…”

Section: Introductionmentioning

confidence: 99%

Polyphenols in the Fermentation Liquid of Dendrobium candidum Relieve Intestinal Inflammation in Zebrafish Through the Intestinal Microbiome-Mediated Immune Response

et al. 2020

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Previous studies of Dendrobium candidum ( D. candidum ), which is mainly distributed in tropical areas, have mainly focused on its functional polysaccharide; the effects of D. candidum polyphenols, the chemical composition of which may be improved by fermentation, have received limited attention, especially in in vivo models, which inevitably involve interactions with intestinal microorganisms. To address this challenge, metagenomic and metabolomic techniques, were applied, and immune factors and mucosal barrier-related proteins were determined to reveal the effects of fermented D. candidum polyphenols (FDC) on intestinal inflammation induced by oxazolone in zebrafish. The results showed that fermentation significantly changed the chemical composition of D. candidum and that FDC significantly improved the intestinal immune index. After the 21st day of FDC intervention, the abundance of Lactobacillus, Faecalibacterium , and Rummeliibacillus increased, but the abundance of the genera Shewanella, Geodermatophilus, Peptostreptococcaceae , and Mycobacterium decreased. At the same time, FDC significantly increased intestinal short-chain fatty acids (SCFAs). In addition, network analysis based on multi-omics indicated that FDC intake leads to changes in intestinal microbiota and intestinal metabolites, resulting in enhanced host immune function. These results indicate that FDC can improve intestinal health by regulating the intestinal microbiota and its metabolites to treat intestinal inflammation and regulate the host immune system. The present research improved our understanding of the utilization of D. candidum polyphenols and provided new evidence for the impacts of fermented D. candidum on host health.

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Dendrobium officinale polysaccharides alleviate colon tumorigenesis via restoring intestinal barrier function and enhancing anti-tumor immune response

Cited by 96 publications

References 52 publications

Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates

Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates

PAMK Relieves LPS-Induced Enteritis and Improves Intestinal Flora Disorder in Goslings

Polyphenols in the Fermentation Liquid of Dendrobium candidum Relieve Intestinal Inflammation in Zebrafish Through the Intestinal Microbiome-Mediated Immune Response

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