Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates

Abstract

Dendrobium officinale, a precious herbal medicine, has been used for a long time in Chinese history. The metabolites of D. officinale, regarded as its effective components to fight diseases, are significantly affected by cultivation substrates. In this study, ultra-performance liquid chromatography mass spectrometry (UPLC-MS/MS) was conducted to analyze D. officinale stems cultured in three different substrates: pine bark (PB), coconut coir (CC), and a pine bark: coconut coir 1:1 mix (PC). A total of 529 metabolites were identified. Multivariate statistical analysis methods were employed to analyze the difference in the content of metabolites extracted from different groups. By the criteria of variable importance in projection (VIP) value ≥1 and absolute log2 (fold change) ≥1, there were a total of 68, 51, and 57 metabolites, with significant differences in content across groups being filtrated out between PB and PC, PB and CC, and PC and CC, respectively. The comparisons among the three groups revealed that flavonoids were the metabolites that fluctuated most. The results suggested the D. officinale stems from the PB group possessed a higher flavonoid content. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that the significantly regulated metabolites were mainly connected with flavonoid biosynthesis. A comprehensive profile of the metabolic differentiation of D. officinale planted in different substrates was provided, which supports the selection of an optimum cultivation substrate for a higher biomass yield of D. officinale.

Comparative Analysis of Nutritional Quality of Dendrobium Officinale Kimura Et Migo After Transplantation of Artificially Cultivated Plants From South to North China During Different Growing Years

“…Untargeted metabolic analysis has been widely used for a comprehensive comparison of detailed metabolite identi cations under different conditions [11,22,23] . In our study, a total of 272 metabolites were detected in the current study, and the number of detections was greater than some studies.…”

“…For example, a work aimed to compare metabolite characteristics between two medicinal Dendrobium found 139 major metabolites from D. o cinale [20] . Others found more metabolites after different treatments, for example, one study shows a total number of 529 metabolites based on different cultivation substrates [23] , another found 3,655 annotated metabolites with Ultraviolet-B radiation treatments, indicating that ultraviolet-B radiation helps to the accumulation of active compounds in D. o cinale. Although different studies showed signi cant differences in the number of metabolites detected, this suggests that environmental factors play an important role in the types and amounts of metabolites.…”

Research Advances in Multi-Omics on the Traditional Chinese Herb Dendrobium officinale

“…This research characterizes the biosynthetic pathways of active compounds in D. officinale , which provides an important basis for its uses as a medicinal material ( Table 1 ; Yuan et al, 2020a ). Transcriptomic analysis was also used to investigate the effects of various factors on the accumulation of active compounds, including the material location, hormones and more ( Chen et al, 2019 ; Ren et al, 2020 ; Zuo et al, 2020 ). Moreover, the potential molecular mechanisms of a variety of physiological processes were analyzed, such as response to stress, flowering and symbiotic relationships ( Wang et al, 2018 ; Zou et al, 2018 ; Li H. et al, 2021 ).…”

“…Bibenzyls (belonging to phenolic compounds) including erianin and gigantol are mainly detected in roots and stems ( Adejobi et al, 2021 ). Moreover, increasing evidence shows that the biosynthesis and accumulation of diverse active compounds are often influenced by various environmental factors such as different cultivation substrates ( Zuo et al, 2020 ) and habitats ( Lei et al, 2018 ). Therefore, it is important to dissect the physiological and molecular mechanisms underlying accumulation of those active compounds during the growth and development of different tissues for breeding by genetic improvement, cultivation and management practices in agriculture.…”

The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats

“…The VIP value determined significantly regulated metabolites between groups ≥ 1, fold change ≥ 1 (17). VIP values were extracted from OPLS-DA results, containing score plots and permutation plots generated using R package MetaboAnalystR.…”