Dendrobii Officinalis, a traditional Chinese edible and officinal plant, accelerates liver recovery by regulating the gut-liver axis in NAFLD mice

Lei, Shanshan; Li, Bo; Chen, Ye‐Hui; He, Xinglishang; Wang, Yuzhi; Yu, Haiqin; Zhou, Fu-Chen; Zheng, Xiang; Chen, Xue; Zhang, Ning-Yu; Shi, Jie; Yan, Ming; Lv, Gui‐Yuan; Chen, Suhong

doi:10.1016/j.jff.2019.103458

Cited by 28 publications

(45 citation statements)

References 21 publications

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“…e histopathological examination of the intestine and kidney was evaluated by hematoxylin and eosin (H&E) staining as previously described [16]. e intestinal and kidney tissues from SD rats were immobilized in 10% formalin solution for 48 hours.…”

Section: Histopathological Examination Of the Intestine And Kidneymentioning

confidence: 99%

“…No liver tissue fragments were found in the turbid liquid of the centrifugal tube. e supernatant was centrifuged at 3,500 r/min and 4°C for 15 min and stored for testing [16]. e activities of liver proteins XOD, ADA, FABP1, and LPL were detected by Epoch2 (Bio-TEK, USA).…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…Mouse and rabbit-specific HRP/DAB (ABC) assay IHC kit was used to detect expression of ABCG2, Caspase-1, TLR4, and NLRP3 in kidney and intestine specimens, and the tissue was counterstained with hematoxylin. In brief, kidney sections were incubated with diluted primary antibodies ABCG2 (1 : 200), Caspase-1 (1 : 200), and NLRP3 (1 : 200) overnight at 4°C, and intestinal sections were incubated with diluted primary antibodies ABCG2 (1 : 200), TLR4 (1 : 200), and NLRP3 (1 : 200) overnight at 4°C [16]. Tissue sections were photographed using a biological microscope.…”

Section: Immunohistochemistry (Ihc) Stainingmentioning

confidence: 99%

“…Dendrobii officinalis (DOF) Caulis are the dried stems of the orchid Dendrobium officinalis Kimura et Migo of the family Orchidaceae. DOF is a traditional Chinese edible plant that has been medicinally used in China for hundreds of years, with a wide range of functions such as protecting against cancer and lowering blood pressure [16]. Prelaboratory studies have found that DOF Chinese herb compound can reduce SUA [13], and it has also been found to effect of regulating blood lipids [17].…”

Section: Introductionmentioning

confidence: 99%

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Effects and Mechanisms of Dendrobium officinalis Six Nostrum for Treatment of Hyperuricemia with Hyperlipidemia

Guo

Chen

Lei

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objectives. Hyperuricemia (HUA) is a disease caused by increased production of uric acid (UA) or reduced excretion of UA in the body. Results of an epidemiological survey show that 60% of patients with HUA have hyperlipidemia (HPA). Dendrobium officinalis (DOF) six nostrum (DOS) is based on the theory of traditional Chinese medicine for the transformation of the traditional Chinese nostrum Si Miao Wan. In this article, we aim to discuss the efficacy and mechanism of DOS in reducing UA and regulating lipid metabolism. The rat model of HUA with HPA was induced by potassium oxonate (PO) combined with high-fat sorghum feed. We monitored the serum UA and blood lipids. Liver xanthine oxidase (XOD), adenosine deaminase (ADA), lipoprotein lipase (LPL), and fatty acid-binding protein (FABP1) activities were measured by enzyme-linked immunosorbent assay (ELISA) after the last administration of DOS. We performed a histopathological examination of rat kidney and intestine. Immunohistochemistry (IHC) was used to detect the expression of renal inflammatory proteins NLRP3 / Caspase-1 and intestinal inflammatory proteins TLR4 / NLRP3. We used western blot for measurement of liver hypoxanthine-guanine phosphoribosyl transferase (HPRT1) protein expression and renal PDZ domain protein kidney 1 (PDZK1) protein expression. DOS administration significantly reduced serum UA, total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-c) level, and improved liver steatosis in the model rat. At the same time, DOS treatment effectively inhibited liver XOD and ADA, increased the level of liver HPRT1, and reduced the production of UA. Additional studies had shown that DOS can restore normal UA excretion function in the intestine and kidney and regulated liver lipids metabolism. IHC and histopathological sections showed that DOS reduced the level of kidney, intestinal inflammatory body (NLRP3, Caspase-1, and TLR4), improved inflammation of the kidney and intestinal tract in rats. DOS is a promising drug that can effectively reduce serum UA and lipid level in the model rat. The mechanism of action may be related to inhibition of UA production, promotion of UA excretion, regulation of lipids metabolism, and anti-inflammatory response.

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Section: Histopathological Examination Of the Intestine And Kidneymentioning

confidence: 99%

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Section: Immunohistochemistry (Ihc) Stainingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects and Mechanisms of Dendrobium officinalis Six Nostrum for Treatment of Hyperuricemia with Hyperlipidemia

Guo

Chen

Lei

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Ear, throat, and lung tissue sections were Hematoxylin and eosin (H&E) staining according to the previously published literatures [26]. The results were assessed by Olympus BX43 light microscope (Olympus, Tokyo, Japan) and the throat pathology of pharyngitis was scored as Table 2 described [27].…”

Section: Hematoxylin and Eosin (Hande) Stainingmentioning

confidence: 99%

Anti-inflammatory effect of Ganluyin, a Chinese classic prescription, in chronic pharyngitis rat model

Chen

Luo

Lei

et al. 2020

BMC Complement Med Ther

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Background: Ganluyin (GLY) is a famous classical prescription with a long history of use as a treatment for inflammatory conditions such as chronic pharyngitis (CP) in many parts of China. However, it has not been developed as a modern pharmaceutic and its anti-inflammatory mechanisms remain unclear. The aim of this study was to assess the anti-inflammatory efficacy of GLY and potential mechanisms in a rat model of CP. Methods: The chemical profile of GLY was analyzed by HPLC-UV. We used a mouse model of ear edema and a rat model of paw edema. Specifically, xylene was used to induce edema on the surface of one ear in mice, and carrageenan was injected subcutaneously into the right hind paws of rats to induce paw edema. The paw thickness, ear weight, and ear perfusion were measured and recorded. The CP model in rats was induced by irritating the throat with 5% ammonia and was used to evaluate the therapeutic efficacy of GLY. Levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE2) were measured by ELISA in serum, and protein expression of cyclooxygenase-2 (COX-2) and nuclear factor kappa-B p65 (NF-κB p65) in the throat were detected by immunohistochemistry and Western blot to evaluate the anti-inflammatory mechanism of GLY. Hematological assays were also conducted. Results: There were four flavonoids identified in GLY: naringin, neohesperidin, baicalin, and wogonoside. The oral administration of GLY showed a significant inhibitory effect on xylene-induced ear swelling and ear blood flow in mice and significantly ameliorated rat right hind paw edema at doses of 6.2 and 12.4 g/kg. Mechanistic studies found that the anti-inflammatory activity of GLY was related to the inhibition of pro-inflammatory cytokines such as IL-1β, IL-6, TNF-α, and PGE2 and that GLY reduced the expression of COX-2 and NF-κB p65 proteins in the throat, attenuated throat injury, and reduced inflammatory exudates. Hematological analysis showed that treatment with GLY prevented increases in white blood cell (WBC), neutrophil (NEUT), lymphocyte (LYMPH) and monocyte (MONO) levels.

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Luteolin alleviates non-alcoholic fatty liver disease in rats via restoration of intestinal mucosal barrier damage and microbiota imbalance involving in gut-liver axis

Liu

Sun

et al. 2021

Archives of Biochemistry and Biophysics

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Dendrobii Officinalis, a traditional Chinese edible and officinal plant, accelerates liver recovery by regulating the gut-liver axis in NAFLD mice

Cited by 28 publications

References 21 publications

Effects and Mechanisms of Dendrobium officinalis Six Nostrum for Treatment of Hyperuricemia with Hyperlipidemia

Effects and Mechanisms of Dendrobium officinalis Six Nostrum for Treatment of Hyperuricemia with Hyperlipidemia

Anti-inflammatory effect of Ganluyin, a Chinese classic prescription, in chronic pharyngitis rat model

Luteolin alleviates non-alcoholic fatty liver disease in rats via restoration of intestinal mucosal barrier damage and microbiota imbalance involving in gut-liver axis

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