Dendroaspis natriuretic peptide and its functions in pig ovarian granulosa cells

Piao, Feng Lian; Park, Sung Hun; Han, Jeong Hee; Cao, Chunhua; Kim, Sung Zoo; Kim, Suhn Hee

doi:10.1016/j.regpep.2003.12.011

Cited by 18 publications

(14 citation statements)

References 24 publications

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“…1A): (1) Oviduct at the small follicle stage (the diameter of all follicles in the ovary was less than 3 mm, OT1); (2) oviduct at the middle follicle stage (a few of 3-6 mm follicles existed in the ovary, OT2); (3) oviduct at the large follicle stage (a few of more than 6 mm follicles existed in the ovary, OT3); (4) oviduct at the corpus rubrum stage (OT4); (5) oviduct at the corpus luteum stage (OT5). Semi-quantitative RT-PCR was performed as described by Piao et al (2004). Briefly, 1 µg total RNA was reverse transcribed at 42 C for 50 min, denatured at 95 C for 2 min, and amplified for 32 (beta-actin) or 35 (ANP, NPRA and NPRC) cycles of denaturation at 94 C of 30 s, primer annealing at 54 C for 30 s, and extension at 72 C for 30 s, with a final extension step of 5 min at 72 C. The amplified products were analyzed by electrophoresis on 1% agarose gels.…”

Section: Rna Isolation and Rt-pcrmentioning

confidence: 99%

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The expression of atrial natriuretic peptide in the oviduct and its functions in pig spermatozoa

Zhang

Hong

Zhou

et al. 2006

Journal of Endocrinology

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Locally synthesized atrial natriuretic peptide (ANP) and its receptors have been found in reproductive tissues of various mammals, and play an important role in the acrosome reaction of human sperm. The objective of the present study was to examine the expression of ANP and its receptors in pig spermatozoa and oviduct, and the effect of ANP on pig spermatozoa function. The expression of ANP and its receptors was analyzed by RT-PCR. Only natriuretic peptide receptors-A (NPRA) mRNA was detected in fresh sperm. While the levels of natriuretic peptide receptors-C (NPRC) mRNA were low with no obvious change among different oviductal phases, the levels of ANP mRNA were high in oviduct(OT)1 , OT3 and OT5, but were very low in OT2. On the other hand, the levels of NPRA mRNA were low in OT1 and OT2, increased in OT3 and reached a maximum in OT4 and OT5. Western blot analysis revealed that the level of ANP was high in OT1, decreased in OT2 and OT3, and arrived at the nadir in OT4 and OT5. The effect of ANP on spermatozoa function was studied by the acrosome reaction and IVF. Incubation with ANP for 1 h significantly induced acrosome reaction of preincubated spermatozoa, and maximal response of acrosome reaction (34·1 2·3%) was achieved at 1 nM ANP treatment. Both C-ANP-(4-23), a selective ligand of NPRC, and caffeine had no effect on the acrosome reaction. The stimulatory effect of ANP on acrosome reaction could be mimicked by the permeable cGMP analog, 8-Br-cGMP. ANP and caffeine had a similar effect on improving the oocytes penetration rate, polyspermy rate and the average number of sperm per penetrated oocyte. Also, ANP treatment had a similar effect on cleavage rate, blastocyst formation rate and the number of cells per blastocyst as that of caffeine treatment. The effects of ANP on the acrosome reaction and the parameters of oocyte penetration could be blocked by cGMP-dependent protein kinase (PKG) inhibitors KT5823 and/or Rp-8-pCPT-cGMPS. These results suggest that the expression of ANP in the oviduct may be involved in the regulation of the acrosome reaction and the fertilising ability of pig spermatozoa, and the PKG pathway possibly participates in the process.

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Section: Rna Isolation and Rt-pcrmentioning

confidence: 99%

“…NM012868). This primer pair predicts 401-bp DNA fragments (Piao et al 2004). The beta-actin primers were based on the mouse sequence (GenBank accession no.…”

Section: Rna Isolation and Rt-pcrmentioning

confidence: 99%

The expression of atrial natriuretic peptide in the oviduct and its functions in pig spermatozoa

Zhang

Hong

Zhou

et al. 2006

Journal of Endocrinology

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“…DNP is a recently isolated peptide that contains 38 amino residues and shares structural and functional properties with the other members of the natriuretic peptide family [2] . Studies about its physiologic functions mainly focus on cardiovascular [3][4][5] , genital [6] , and urinary systems [7] . Evolutionary studies [8] have suggested the presence of DNP-like immunoreactivity in rat colon, and the DNP-like molecule may control colonic motility as a local regulator.…”

Section: Introductionmentioning

confidence: 99%

Dendroaspis natriuretic peptide relaxes gastric antral circular smooth muscle of guinea-pig through the cGMP/cGMP-dependent protein kinase pathway

Cheng¹,

Cai²,

Gu³

et al. 2008

WJG

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AIM:To systematically investigate if cGMP/cGMPdependent protein kinase G (PKG) signaling pathway may participate in dendroaspis natriuretic peptide (DNP)-induced relaxation of gastric circular smooth muscle. METHODS:The content of cGMP in guinea pig gastric antral smooth muscle tissue and perfusion solution were measured using radioimmunoassay; spontaneous contraction of gastric antral circular muscles recorded using a 4-channel physiograph;

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“…Dendroaspis natriuretic peptide (DNP), a 38-amino residue peptide that contains a disulfide ring structure and was originally isolated from the venom of Green Mamba [6], is a new member of the natriuretic peptide family with a structure and function similar to other members. Studies about its physiological functions are limited and mainly focus on the cardiovascular [7], urinary [8], and genital systems [9]. Moreover, little is known about the functional role of DNP in gastrointestinal cells.…”

Section: Introductionmentioning

confidence: 99%

“…Studies about its physiological functions are limited and mainly focus on the cardiovascular [7], urinary [8], and genital systems [9]. Moreover, little is known about the functional role of DNP in gastrointestinal cells.…”

mentioning

confidence: 99%

Effects of Dendroaspis Natriuretic Peptide on Calcium-Activated Potassium Current and Its Mechanism

et al. 2008

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Abstract:In this study, we sought to investigate the effect of dendroaspis natriuretic peptide (DNP) on calcium-activated potassium current (I K(Ca) ) and its mechanism in gastric antral circular smooth muscle cells (SMCs) using the whole-cell patchclamp technique. DNP concentration-dependently increased macroscopic I K(Ca) and spontaneous transient outward currents (STOCs) in freshly isolated guinea pig gastric antral circular SMCs. The effects of DNP on I K(Ca) and/or STOCs were not blocked by applying calcium-free bath solution or the ryanodine receptor (RyR) antagonist ryanodine (10 µM), but they were inhibited by the inositol triphosphate receptor (IP 3 R) inhibitor heparin or the guanylate cyclase inhibitor LY83583. Moreover, a DNP-induced increase in STOCs was potentiated by the cyclic guanosine monophosphate (cGMP)-sensitive phosphoesterase inhibitor zaprinast. In conclusion, our results suggest that DNP increases I K(Ca) in gastric antral circular SMCs by increasing cGMP production and activating IP 3 Rs.Key words: dendroaspis natriuretic peptide, calcium-activated potassium channels, Ca 2+ -induced Ca 2+ release, inositol 1,4,5-trisphosphate receptors, ryanodine receptor. Dendroaspis natriuretic peptide (DNP), a 38-amino residue peptide that contains a disulfide ring structure and was originally isolated from the venom of Green Mamba [6], is a new member of the natriuretic peptide family with a structure and function similar to other members. Studies about its physiological functions are limited and mainly focus on the cardiovascular [7], urinary [8], and genital systems [9]. Moreover, little is known about the functional role of DNP in gastrointestinal cells. Kim et al. [10] have for the first time demonstrated that DNP is present in rat colon and can control colonic motility as a local regulator. We have found that NPs can also regulate gastric motility [11][12][13][14]. Our further study indicates that the regulatory effect of DNP on spontaneous contraction in gastric antral circular SMCs occurs via a cGMP-dependent pathway [15]. However, the underlying mechanisms are unknown. Until now, there have been few studies about the relationship between DNP-induced increase of I K(Ca) and IP 3 Rs. In our study [16], we found that SNP increased by cGMP via IP 3 Rs. NPs are similar to nitrc oxide which is a cGMP generation system in the living body, and their physiological function exhibits very important in life science. Considering that calcium-activated potassium channels play an important role in the regulation of contractility in gastric SMCs, in this study we first intended to investigate whether DNP could affect I K(Ca) in gastric antral circular SMCs using the conventional whole-cell patch clamp technique, and then to determine the potential involvement of calcium mobilization in the effect of DNP. Moreover, the effect of cGMP on DNP-induced change of I K(Ca) was observed.

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Dendroaspis natriuretic peptide and its functions in pig ovarian granulosa cells

Cited by 18 publications

References 24 publications

The expression of atrial natriuretic peptide in the oviduct and its functions in pig spermatozoa

The expression of atrial natriuretic peptide in the oviduct and its functions in pig spermatozoa

Dendroaspis natriuretic peptide relaxes gastric antral circular smooth muscle of guinea-pig through the cGMP/cGMP-dependent protein kinase pathway

Effects of Dendroaspis Natriuretic Peptide on Calcium-Activated Potassium Current and Its Mechanism

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