Demonstration of virulent genes within Listeria and Klebsiella isolates contaminating the export quality frozen shrimps

Abstract

In the previously studied export quality shrimp samples, presence of a range of pathogenic bacteria including Listeria spp. and Klebsiella spp. were detected. The presence of eae gene in Escherichia coli, aero specific gene in Aeromonas spp., and sodB gene in Vibrio spp. were observed which might confer the associated virulence. Present study further attempted to detect the existence of virulent genes in Listeria spp. and Klebsiella isolates from the same shrimp samples through the gene specific polymerase chain reaction (PCR). Visualization of bands of iap (457 bp) and gyrB2 (711 bp) genes after PCR amplification referred the presence of virulence strains of Listeria spp. and Klebsiella spp., respectively in the export quality shrimp samples. Together with the previous findings of virulence genes of several pathogenic bacteria, the outcomes of current study further conferred the possible detrimental impact of the export quality frozen shrimps as and when consumed.

Microbiological quality of commonly consumed street foods in Bangladesh

“…Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, hemorrhagic colitis, bloodstream infection, meningitis, joint infection, kidney failure, paralysis, miscarriage, etc. (Noor and Munna, 2015;Noor and Feroz, 2015;Noor et al, 2015a;Khan et al, 2015Khan et al, , 2014Toyofuku, 2014;Todd, 2014;Acharjee et al, 2013a;Noor et al, 2014Noor et al, , 2013aNoor et al, , 2013bRoy et al, 2013;Nipa et al, 2011;Scallan et al, 2011). Foods are usually contaminated with various bacterial species and fungi during harvesting; processing; and using of associated machines, utensils, water, etc.…”

Food safety in Bangladesh: A microbiological perspective

“…Lunching the facilities in the research laboratories for conducting sophisticated experiments relating molecular methods used to detect and identify foodborne pathogens are recommended (46).…”

General Perspectives on Water and Fluid Borne Microorganisms in Bangladesh

“…Developing countries like Bangladesh is more venturesome due to the lack of knowledge of handling, storing food or water raw samples 'from production to consumer'; besides, poor hygiene of maintaining food and water, human induced adulterated food, fabrication etc. are also playing roles to spread several diseases or even outbreaks to epidemics [10][11][12][13] . So, to keep control the human's basic rights on food safety the 'Food Safety Act 2013' has also been enacted in Bangladesh through repealing and re-enacting the existing outdated laws in these regards [14][15][16] .…”