Demonstration of the “5.8 S” ribosomal sequence in HeLa cell ribosomal precursor RNA

Maden, B.E.H.; Robertson, J. S.

doi:10.1016/0022-2836(74)90145-4

Cited by 72 publications

(26 citation statements)

References 13 publications

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“…1 b) was examined by fingerprinting analysis, using 32P labelled RNA and combined Tr RNase plus alkaline phosphatasc digestion [lo] . In such fingerprints many unique 28 S, 5.8 S and transcribed spacer oligonucleotides of 32 S RNA are directly resolved [2,7]. The main band of 32 S RNA revealed all three classes of oligonucleotides, as previously reported [ 2,7,11] , (fig.2a, Key).…”

Section: Methods and Resultssupporting

confidence: 61%

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Maturation of 5.8 S RNA in HeLa cells

Khan

Maden

1976

FEBS Letters

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Section: Methods and Resultssupporting

confidence: 61%

“…HeLa cells were grown in monolayers and were labelled with "PO4 as described previously [2,7] . Actinomycin D (4 pg/ml) was added 30 min before harvesting.…”

Section: Methods and Resultsmentioning

confidence: 99%

Maturation of 5.8 S RNA in HeLa cells

Khan

Maden

1976

FEBS Letters

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“…It is encoded by the major ribosomal DNA (rDNA) ' and is co-transcribed with 18 S and 28 S RNA within ribosomal precursor RNA 5,6. It is cleaved into a separate entity during ribosome maturation and remains noncovalently attached to 28 S RNA1'.…”

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs

Khan

Maden

1977

Nucl Acids Res

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Nucleotide sequences of the 5.8 S ribosomal RNAs from HeLa cells, Xenopus laevis and chick embryo fibroblasts were compared. Xenopus laevis 5.8 S RNA differs from that of HeLa cells in four internal positions and at the 3' end of the molecule.Chick 5.8 S RNA differs from that of HeLa cells in two positions.Six out of the seven interspecies differences are due to base substitutions.The other difference is due to the presence of an extra nucleotide, internally located, within the Xenopus 5.8 S sequence.

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“…5.8S rRNA was prepared as described by Maden & Robertson (1974). In most of the experiments described here it was purified by re-running on a second 10% (w/v) to 25% (w/v) sucrose gradient in 0.1 MLiCI/0.01 M-EDTA/0.01 M-Tris/HCl (pH 7.4)/sodium dodecyl sulphate (0.2%, w/v) for 24h at 26000rev./ min, at 20°C in the Beckman SW 27 rotor.…”

Section: Methods Preparation Ofrnamentioning

confidence: 99%

“…Knowledge of the conformation of isolated 5.8S rRNA should provide a basis for examining which nucleotides are involved in the specific interaction with 28 S rRNA. Moreover 5.8S rRNA is encoded within the major 'ribosomal DNA' (Speirs & Birnstiel, 1974; and is transcribed within the same 45S precursor rRNA molecule as 18 S and 28 S rRNA (Maden & Robertson, 1974;Nazar et al, 1975a). Vol.…”

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Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite

Kelly¹,

Goddard²,

Maden³

1978

Biochemical Journal

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The reaction of HeLa-cell 5.8 S rRNA with NaHSO3 under conditions in which exposed cytidine residues are deaminated to uridine was studied. It was possible to estimate the reactivities of most of the 46 cytidine residues in the nucleotide sequence by comparing 'fingerprints' of the bisulphite-treated RNA with those of untreated RNA. The findings were consistent with the main features of the secondary-structure model for mammalian 5.8 S rRNA proposed by Nazar, Sitz & Busch [J. Biol. Chem. (1975) 250,[8591][8592][8593][8594][8595][8596][8597]. Five out of six regions that are depicted in the model as single-stranded loops contain cytidine residues that are reactive towards bisulphite at 25°C (the other loop contains no cytidine). The cytidine residue nearest to the 3'-terminus is also reactive. Several cytidine residues that are internally located within proposed double-helical regions show little or no reactivity towards bisulphite, but the cytidine residues of several C. G pairs at the ends of helical regions show some reactivity, and one of the proposed loops appears to contain six nucleotides, rather than the minimum of four suggested by the primary structure. Two cytidine residues that are thought to be 'looped out' by small helix imperfections also show some reactivity.

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Demonstration of the “5.8 S” ribosomal sequence in HeLa cell ribosomal precursor RNA

Cited by 72 publications

References 13 publications

Maturation of 5.8 S RNA in HeLa cells

Maturation of 5.8 S RNA in HeLa cells

Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs

Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite

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