Demonstration of Different Regional Distributions of Calcineurin Subunits using Monoclonal Antibodies

Matsui, Hideki; Itano, Toshifumi; Etoh, S; Tokuda, Masaaki; Wang, Jerry H.; Hatase, Osamu

doi:10.1007/978-1-4684-5679-0_40

Cited by 6 publications

(2 citation statements)

References 13 publications

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“…The presence of aluminum in soil is a natural phenomenon, but current studies suggest that the natural environment is significantly exposed to elevated levels of this mineral [29], whereas excessive accumulation of aluminum in brain tissue manifests itself primarily by memory deficit and posture dysfunction [29]. Vegetables cultivated in areas contaminated with heavy metals may include large amounts of aluminum, but it seems that the main source of human exposure comes from the public water supply [29,30]. For the general population, the intake of aluminum from food is 7.2 mg/day for females and 8.6 mg/day for males on average [31].…”

Section: Discussionmentioning

confidence: 99%

Mineral Composition and Antioxidant Potential of Coffee Beverages Depending on the Brewing Method

Janda

Jakubczyk

Baranowska-Bosiacka

et al. 2020

Foods

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Coffee, being one of the world’s most popular beverages, is a rich source of dietary antioxidants. The aim of this study was to determine the mineral content and antioxidant activity as well as acidity of coffee beverages depending on the brewing technique. We tested coffee brews made and served at a popular urban coffee shop (Szczecin, Poland). Five coffee brewing techniques were used: Aeropress, drip, espresso machine, French press, and simple infusion. Our findings showed that the brewing method had a significant effect on all parameters tested in the study. The antioxidant activity of the beverages was high (31%–42% inhibition of DPPH (2,2-diphenyl-1-picrylhydrazyl); reduction potential from 3435.06 mol Fe3+/mL to 4298.19 mol Fe3+/mL). Polyphenolic content ranged from 133.90 g (French press) to 191.29 g of gallic acid/L (Aeropress brew), depending on the coffee extraction method. Mineral content was also found to differ between brewing methods. Coffees prepared by simple infusion and Aeropress provided a valuable source of magnesium, manganese, chromium, cobalt, and potassium, whereas the drip brew was found to be a good source of silicon.

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Section: Discussionmentioning

confidence: 99%

Mineral Composition and Antioxidant Potential of Coffee Beverages Depending on the Brewing Method

Janda

Jakubczyk

Baranowska-Bosiacka

et al. 2020

Foods

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“…Calcineurin (CaN), also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent serine/threonine phosphatase physiologically activated by Ca 2+ . It is also highly expressed in the central nervous system [ 31 ] and responsible for the dephosphorylation of p-CaMKII. CaN is composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Herbal formula GAPT prevents beta amyloid deposition induced Ca2+/Calmodulin-dependent protein kinase II and Ca2+/Calmodulin-dependent protein phosphatase 2B imbalance in APPV717I mice

Shi

Zhang

Yin

et al. 2016

BMC Complement Altern Med

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BackgroundSynaptic dysfunction is one of the pathological characteristics of Alzheimer's disease (AD), which is directly related to the progressive decline of cognitive function. CaMKII and CaN have been found to play important roles in memory processes and synaptic transmission. So present study aimed to elucidate relationships between CaMKII, CaN and cognitive decline in APPV717I mice, and to reveal whether the cognitive improving effects of GAPT is conducted through rebalance CaMKII and CaN.MethodsThree-month-old-male APPV717I mice were randomly divided into ten groups (n = 12 per group) and received intragastrically administrated vehicle, donepezil or different doses of herbal formula GAPT for 8 or 4 months. Three-month-old male C57BL/6 J mice was set as vehicle control.ResultsImmunohistochemistry analysis showed that there were CaMKII expression decrease in the CA1 region of APPV717I transgenic mice, while the CaMKII expression of donepezil or GAPT treated transgenic mice were all increased. And there were CaN expression increase in the brain cortex of APPV717I transgenic mice, while there were decrease of CaN expression in donepezil or GAPT treated transgenic group. Western blot analysis showed the similar expression pattern without significant difference.ConclusionGAPT extract have showed effectiveness in activating the expression of CaMKII and inhibiting the expression of CaN either before or after the formation of amyloid plaques in the brain of APPV717I transgenic mice, which may in certain way alleviated neuron synaptic dysfunction in AD.

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Immunosupressants and calcineurin inhibitors, cyclosporin A and FK506, reversibly inhibit epileptogenesis in amygdaloid kindled rat

et al. 1994

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Demonstration of Different Regional Distributions of Calcineurin Subunits using Monoclonal Antibodies

Cited by 6 publications

References 13 publications

Mineral Composition and Antioxidant Potential of Coffee Beverages Depending on the Brewing Method

Mineral Composition and Antioxidant Potential of Coffee Beverages Depending on the Brewing Method

Herbal formula GAPT prevents beta amyloid deposition induced Ca2+/Calmodulin-dependent protein kinase II and Ca2+/Calmodulin-dependent protein phosphatase 2B imbalance in APPV717I mice

Immunosupressants and calcineurin inhibitors, cyclosporin A and FK506, reversibly inhibit epileptogenesis in amygdaloid kindled rat

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