Demonstration of Chlamydia pneumoniae in Patients with Chronic Pharyngitis

Falck, Göran; Engstrand, I; Gad, Ahmed; Gnarpe, Judy; Gnarpe, Håkan; Laurila, Aino

doi:10.3109/00365549709035899

Cited by 41 publications

(26 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several reports have indicated that chronic C. pneumoniae infections may be associated with coronary heart disease, atherosclerosis, and asthma (4,9,12,17). Although the acute infection is usually self-limiting, prolonged respiratory symptoms are sometimes a feature, and in such cases C. pneumoniae has been difficult to eradicate with antibiotics (6,7). The optimal choice of antibiotic treatment and dosing regimen still needs to be defined.…”

mentioning

confidence: 99%

In Vitro Pharmacodynamics of the New Ketolides HMR 3004 and HMR 3647 (Telithromycin) against Chlamydia pneumoniae

Gustafsson

Hjelm

Cars

2000

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The ketolides HMR 3004 and HMR 3647 (telithromycin) are a new class of macrolides that have a potential clinical efficacy against intracellular pathogens. The objectives of this study were to investigate the MIC, minimum bactericidal concentration, and time-dependent killing of two Chlamydia pneumoniae strains of the two ketolides. The killing effect was also studied with a newly developed intracellular in vitro kinetic model. Furthermore, HMR 3647 was studied for the effect of a subinhibitory concentration of 0.5 times the MIC after a preexposure of 10 times the MIC during 12 h. The MICs for both strains were 0.0039 and 0.0156 mg/liter for HMR 3004 and HMR 3647, respectively. Killing with 10 times the MIC was time dependent, increasing from a 1-log-unit decrease in the number of inclusions per well at 48 h to a maximal effect of 2.8-log-unit decrease after 96 h. A preexposure of 10 times the MIC of HMR 3647 for 12 h followed by a subinhibitory concentration of 0.5 times the MIC increased the killing effect to a 1.2-log-unit reduction in inclusions per well. An exposure for 12 h gave poor reduction of inclusions, while a static dose of 10 times the MIC for 72 h showed a 2.2-log-unit reduction in inclusions per well. In the kinetic model, a small number of inclusions were detected after 72 h by one exposure of 10 times the MIC. Regrowth could not be detected after 120 h. The ketolides HMR 3004 and HMR 3647 have bactericidal activity and show a significant sub-MIC effect on the intracellular pathogen C. pneumoniae.Chlamydia pneumoniae is an obligate intracellular bacterium with a growth cycle of 72 h. It is a widespread human pathogen causing a variety of community-acquired respiratory tract infections in adults and children. Several reports have indicated that chronic C. pneumoniae infections may be associated with coronary heart disease, atherosclerosis, and asthma (4,9,12,17). Although the acute infection is usually self-limiting, prolonged respiratory symptoms are sometimes a feature, and in such cases C. pneumoniae has been difficult to eradicate with antibiotics (6, 7). The optimal choice of antibiotic treatment and dosing regimen still needs to be defined.Pharmacodynamic studies are important tools for the determination of optimal dosing regimens of antimicrobial agents. The ketolides, a new class of macrolides characterized by a 3-keto function, which gives a strong acid stability (3), act by inhibiting bacterial protein synthesis. The ketolides have shown in vitro activity against a broad range of respiratory tract pathogens (1, 2, 15; I. Odenholt, E. Löwdin, and O. Cars, Abstr. 39th Intersci. Conf. Antimicrob. Agents Chemother., abstr. 540, 1999). Although macrolide antibiotics show long postantibiotic effects, their antibacterial activity is not concentration dependent. The major determinant of efficacy is the time that free drug concentrations are greater than the MIC (5). The pharmacodynamics of the ketolides is less well known. However, significant postantibiotic effect and postantibiotic sub-MIC eff...

show abstract

mentioning

confidence: 99%

In Vitro Pharmacodynamics of the New Ketolides HMR 3004 and HMR 3647 (Telithromycin) against Chlamydia pneumoniae

Gustafsson

Hjelm

Cars

2000

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Chronic and persistent respiratory infections associated with or caused by Cp are known to occur, such as chronic pharyngitis [17] and relapsing bronchitis together with pharyngitis and fatigue [18]. Traditionally, chronic bacterial infection has been considered a significant factor in the etiology and pathogenesis of acute sinusitis.…”

Section: Discussionmentioning

confidence: 99%

Chlamydophila pneumoniae in chronic rhinosinusitis

Edvinsson¹,

Asplund²,

Hjelm³

et al. 2006

Acta Oto-Laryngologica

View full text Add to dashboard Cite

show abstract

“…The microorganism has been shown to cause chronic pharyngitis (19) and possibly chronic rhinitis (20). It has been shown that C. trachomatis can be found in the nasopharynx of small children with neonatal infections (21).…”

Section: Discussionmentioning

confidence: 99%

Association of Chlamydia pneumoniae with Otitis Media in Children

Falck

Engstrand²,

Gnarpe³

et al. 1998

Scandinavian Journal of Infectious Diseases

View full text Add to dashboard Cite

Chlamydia pneumoniae is a common cause of acute and persistent respiratory tract infections. The prevalence of C. pneumoniae was studied using the polymerase chain reaction (PCR) in throat swabs from 85 consecutive children with respiratory tract infections and 86 healthy children. In retrospect, it became evident that this study was conducted in the midst of a local C. pneumoniae epidemic. 38 (45%) of the sick children and 5 (5.7%) of the healthy children were positive for C. pneumoniae by PCR. 26 of the sick children (mean age 6.4 years) were found to have otitis media either at the time of examination or shortly thereafter. Six of 9 children with acute otitis media were PCR positive for C. pneumoniae and 7 of the 9 had specific antibody responses indicating active infection. 10 of 17 children diagnosed as having otitis media with effusion were found to be positive for C. pneumoniae by PCR. Seven children had or developed persistent otitis media with effusion. Chlamydia pneumoniae was demonstrated by PCR from the middle ear fluid in 1 of the children. The results obtained from this study indicate that C. pneumoniae may be involved in the aetiology of otitis media.

show abstract

Demonstration of Chlamydia pneumoniae in Patients with Chronic Pharyngitis

Cited by 41 publications

References 30 publications

In Vitro Pharmacodynamics of the New Ketolides HMR 3004 and HMR 3647 (Telithromycin) against Chlamydia pneumoniae

In Vitro Pharmacodynamics of the New Ketolides HMR 3004 and HMR 3647 (Telithromycin) against Chlamydia pneumoniae

Chlamydophila pneumoniae in chronic rhinosinusitis

Association of Chlamydia pneumoniae with Otitis Media in Children

Contact Info

Product

Resources

About