Demonstration of amelogenin in the enamel‐free cusps of rat molar tooth germs: Immunofluorescent and immunoelectron microscopic studies

Inai, Tetsuichiro; Nagata, Kazuhiro; Kukita, Toshio; Kurisu, Kojiro

doi:10.1002/ar.1092330413

Cited by 14 publications

(22 citation statements)

References 19 publications

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“…Further, the CX 43 expression in preameloblasts and preodontoblasts diminished abruptly at the initial calcification in both tissues, enamel and dentine. This was seen typically in the incisor, and also in the first molar cusps, where the region has been known as enamel-free area, but the secretion of enamel matrix protein has been shown by immunohistochemistry (Inai et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of connexin 43 in the developing tooth germ of rat

1995

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Distribution of gap junction protein in maxillary tooth germs of 1-day-old rats was examined by immunohistochemistry, using an affinity-purified antibody specific to residues 360-376 of rat connexin (CX) 43. In 1-day-old rats, the maxillary second molar formed the shape of the cusp, but neither dentine nor enamel was formed between the cells of the dental papilla and the inner enamel epithelium. In the tooth germ, CX 43 was expressed in the cells of the stratum intermedium and the inner enamel epithelium. Labelling in the stratum intermedium was extensive and showed an increasing gradient from peripheral to cuspal regions. CX 43 detected in the inner enamel epithelium was at cell surfaces facing the interface between the dental papilla and the inner enamel epithelium. The cells of the dental papilla and the inner enamel epithelium began differentiation as odontoblasts and secretory ameloblasts respectively, in the cusps of the first molars, where predentine and dentine were formed but enamel matrix was not secreted. CX 43 was present in the stratum intermedium, inner enamel epithelium, preodontoblasts, odontoblasts and subodontoblasts. The incisors showed the most advanced stage of development, where the enamel matrix and calcified dentine were formed in the labial part of the teeth. The CX 43 epitope was seen in the stratum intermedium, inner enamel epithelium, preameloblasts, preodontoblasts, odontoblasts, and subodontoblasts. Immunolabelling was more extensive in the stratum intermedium and subodontoblasts than in preameloblasts, preodontoblasts, and odontoblasts. The immunolabelling in preameloblasts and predontoblasts was accumulated at cell surfaces facing the predentine.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of connexin 43 in the developing tooth germ of rat

1995

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“…Addison and Appleton (1921), Mellanby (1939), Gaunt (1956), and Cohn (1957) concluded that the cells of the inner enamel epithelium adjacent to enamel-free regions did not differentiate into secretory ameloblasts and consequently failed to produce enamel. However, more recent investigations have demonstrated that the epithelial cells over the enamel-free areas elaborate a thin, noncontinuous layer of organic matrix which contains amelogenin (Nakamura et al, 1991;Inai et al, 1992), which becomes mineralized by deposition of fine crystals (Sakakura et al, 1989) and which on demineralization leaves an organic residue devoid of collagen (Sakakura et al, 1989). Contrary to Sutcliffe and Owens (1980), who with the SEM were able to observe what seemed to be remnants of such an enamel layer on the EFA of rat molars, we could not find it on molars of 14-day-old mice.…”

Section: Enamel Distributionmentioning

confidence: 98%

Crown morphology, enamel distribution, and enamel structure in mouse molars

Lyngstadaas¹,

Møinichen²,

Risnes³

1998

Anat. Rec.

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Background: Biomolecular research and genetic manipulations have stressed the importance of thorough knowledge of normal organ morphology. Mouse molar teeth are convenient models for studying basic interactions in organ development and morphogenesis. The aim of the present study was to provide basic information on their morphology.Methods: Intact and sectioned/ground molars of mice of various ages were observed with SEM.Results: Enamel-free areas (EFA) were present on cusp tips at time of eruption. The dominating structural configuration in enamel was prism decussation in inner enamel and parallel prisms in outer enamel. Prism decussation tended to be absent at cusp ridges and in the bottom of grooves. In the former location, the distinction between prisms and interprism was often obscured in the middle enamel zone due to decreased difference in orientation of their crystals. A thin layer of enamel, often aprismatic, covered the distal aspect of cusps in maxillary molars and the mesial aspect of cusps in mandibular molars. The enamel abutting on EFA was often aprismatic. Aprismatic enamel exhibited incremental lines with a periodicity of about 1 m and was often traversed by cracks. The enamel surface was porous in the bottom of grooves. Parts of mouse molar enamel were incompletely mineralized at the time of eruption.Conclusions: SEM is a convenient method for combined studies of crown morphology and enamel structure. Based on morphological criteria, a modification of the cusp nomenclature is proposed. Enamel thickness and structure in mouse molars show regional variations. Fundamental similarities exist between mouse molar cusps and mouse incisors. Mouse molar enamel undergoes posteruptive maturation. Anat.

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“…21,27,28 It also induced hard tissue (dentin-like) formation when used as capping materials for exposed dental pulp in adult pigs. 20,29,30 …”

Section: Introductionmentioning

confidence: 99%

Root maturation and dentin–pulp response to enamel matrix derivative in pulpotomized permanent teeth

et al. 2014

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The success of pulpotomy of young permanent teeth depends on the proper selection of dressing materials. This study aimed to evaluate the histological and histomorphometric response of dentin–pulp complex to the enamel matrix derivative (Emdogain® gel) compared to that of calcium hydroxide when used as a pulp dressing in immature young permanent dogs’ teeth. Dentin-like tissues bridging the full width of the coronal pulp at the interface between the injured and healthy pulp tissues were seen after 1 month in both groups. With time, the dentin bridge increased in thickness for calcium hydroxide but disintegrated and fully disappeared for Emdogain-treated group. Progressive inflammation and total pulp degeneration were only evident with Emdogain-treated group. The root apices of Emdogain-treated teeth became matured and closed by cementum that attached to new alveolar bone by a well-oriented periodontal ligament. In young permanent dentition, Emdogain could be a good candidate for periodontium but not dentino–pulpal complex regeneration.

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Demonstration of amelogenin in the enamel‐free cusps of rat molar tooth germs: Immunofluorescent and immunoelectron microscopic studies

Cited by 14 publications

References 19 publications

Immunohistochemical localization of connexin 43 in the developing tooth germ of rat

Immunohistochemical localization of connexin 43 in the developing tooth germ of rat

Crown morphology, enamel distribution, and enamel structure in mouse molars

Root maturation and dentin–pulp response to enamel matrix derivative in pulpotomized permanent teeth

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