Demonstration of a Mid-Infrared Cavity Enhanced Absorption Spectrometer for Breath Acetone Detection

Ciaffoni, Luca; Hancock, Gus; Harrison, Jeremy J.; Helden, J. H. van; Langley, Cathryn E.; Peverall, R.; Ritchie, Grant A. D.; Wood, Simon

doi:10.1021/ac3031465

Cited by 60 publications

(42 citation statements)

References 26 publications

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“…Furthermore, also broadband absorbing molecules, such as acetone [152], can be detected in breath samples with a portable and compact system. For example, Hancock et al have demonstrated lab-based mid-and near-IR CEAS systems for breath acetone detection [98,101]. Most recently, the same group have reported on a portable and compact device for measuring acetone in breath samples; the device features a 7 cm long high-finesse optical cavity that is coupled to a miniature adsorption pre-concentrator containing 0.5 g of polymer material [102].…”

Section: Opportunitiesmentioning

confidence: 99%

Laser spectroscopy for breath analysis: towards clinical implementation

et al. 2018

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Detection and analysis of volatile compounds in exhaled breath represents an attractive tool for monitoring the metabolic status of a patient and disease diagnosis, since it is non-invasive and fast. Numerous studies have already demonstrated the benefit of breath analysis in clinical settings/applications and encouraged multidisciplinary research to reveal new insights regarding the origins, pathways, and pathophysiological roles of breath components. Many breath analysis methods are currently available to help explore these directions, ranging from mass spectrometry to laser-based spectroscopy and sensor arrays. This review presents an update of the current status of optical methods, using near and mid-infrared sources, for clinical breath gas analysis over the last decade and describes recent technological developments and their applications. The review includes: tunable diode laser absorption spectroscopy, cavity ring-down spectroscopy, integrated cavity output spectroscopy, cavity-enhanced absorption spectroscopy, photoacoustic spectroscopy, quartz-enhanced photoacoustic spectroscopy, and optical frequency comb spectroscopy. A SWOT analysis (strengths, weaknesses, opportunities, and threats) is presented that describes the laser-based techniques within the clinical framework of breath research and their appealing features for clinical use.

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Section: Opportunitiesmentioning

confidence: 99%

Laser spectroscopy for breath analysis: towards clinical implementation

et al. 2018

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“…Figure 3 shows a simulated absorption profile of 500 ppbv acetone and 200 ppbv ethanol in the region of 1150-1300 cm´1 for a path length of 1 km at a pressure of 1 atm. The inset in the top right corner shows the region of 1216.5 cm´1 and 1220 cm´1 that was proposed for breath acetone analysis earlier [18]. Although this wavelength region was chosen because of the minimal spectroscopic interference from water and CO 2 , the contribution of ethanol results in an elevated absorption line without any significant lineshape change.…”

Section: Calibration and Detection Limitmentioning

confidence: 99%

“…Overall, external cavity quantum cascade lasers (EC-QCLs) [13][14][15] represents a light source with high power, broad tuning range, narrow linewidth, and rapid wavelength tuning for the sensitive and accurate detection of trace gases like in breath analysis [16,17]. Examples of applications using EC-QCLs in narrowband absorption include detection of exhaled acetone [18,19], ammonia [20] and nitric oxide [21].…”

Section: Introductionmentioning

confidence: 99%

Influence of Ethanol on Breath Acetone Measurements Using an External Cavity Quantum Cascade Laser

et al. 2016

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Broadly tunable external cavity quantum cascade lasers (EC-QCLs) in combination with off-axis integrated cavity enhanced spectroscopy (OA-ICOS) provide high molecular gas sensitivity and selectivity. We used an EC-QCL in the region of 1150-1300 cm´1 in both broadband scan mode, as well as narrow scanning mode around 1216 cm´1, respectively, for detection of acetone in exhaled breath. This wavelength region is essential for accurate determination of breath acetone due to the relative low spectral influence of other endogenous molecules like water, carbon dioxide or methane. We demonstrated that ethanol has a strong spectroscopic influence on the acetone concentration in exhaled breath, an important detail that has been overlooked so far. An ethanol correction is proposed and validated with the reference measurements from a proton-transfer reaction mass spectrometer (PTR-MS) for the same breath samples from ten persons. With the ethanol correction, both broadband and narrowband molecular spectroscopy represent an attractive way to accurately assess the exhaled breath acetone. The importance of considering spectroscopic ethanol influence is essential, especially for the narrowband scans, (e.g., 1216 cm´1), for which the error in determining the acetone concentrations can rise up to 39% if it is not considered.

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“…According to the experiment performed, the matrix factor value obtained for the QCL was 101.376%, meaning that at this concentration, there was ions enhancement, although not significantly, the value was near 100% [10][11][12][13]. The matrix factor value obtained in the high concentration was 85.053%, meaning that there was ions suppression [14,15].…”

Section: Selectivity Testmentioning

confidence: 95%

Analytical Validation of Clopidogrel in Human Plasma Through Ultrahighperformance Liquid Chromatography-Tandem Mass Spectrometry

Harahap

Maysyarah

2017

Int J App Pharm

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Objective: This study developed a sensitive, selective, and valid method for analyzing clopidogrel in human plasma using liquid chromatographytandem mass spectrometry (LC-MS/MS). Methods:The chromatography separation was performed on the waters ACQUITY UPLC Class BEH C 18 1.7 µm (2.1 × 100 mm) column, consisting of 0.1% formic acid in water and 0.1% formic acid in acetonitrile (30-70) as the mobile phase, and an isocratic elution with a flow rate of 0.2 mL/minutes. The mass detection was performed using a Waters Xevo TQD equipped with positive electrospray ionization in the multiple reaction monitoring modes. The clopidogrel was detected at m/z 322.086>212.097 and irbesartan as an internal standard at m/z 429.233>207.131. The sample was prepared with protein precipitation method using acetonitrile, vortex mixed for 10 minutes, and centrifuged at 13,000 rpm for 20 minutes.Results: This method showed a linear result at the concentration range of 0.2-10 ng/ml with r≥0.9997. Conclusions:The developed method provides sensitivity, linearity, precision, and accuracy and is suitable for analysis of clopidogrel in human plasma using LC-MS/MS.

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Demonstration of a Mid-Infrared Cavity Enhanced Absorption Spectrometer for Breath Acetone Detection

Cited by 60 publications

References 26 publications

Laser spectroscopy for breath analysis: towards clinical implementation

Laser spectroscopy for breath analysis: towards clinical implementation

Influence of Ethanol on Breath Acetone Measurements Using an External Cavity Quantum Cascade Laser

Analytical Validation of Clopidogrel in Human Plasma Through Ultrahighperformance Liquid Chromatography-Tandem Mass Spectrometry

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