Demonstrating the involvement of an active efflux mechanism in the intestinal absorption of chlorogenic acid and quinic acid using a Caco-2 bidirectional permeability assay

Mortelé, Olivier; Jörissen, Jennifer; Spacova, Irina; Lebeer, Sarah; Nuijs, Alexander L.N. van; Hermans, Nina

doi:10.1039/d0fo02629h

Cited by 25 publications

(14 citation statements)

References 35 publications

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“…32,38 More recently, the presence of an active efflux transport which leads to low intestinal absorption in a Caco-2 cell model was demonstrated. 39 In addition, our data are also supported by the results obtained using another dynamic Caco-2 model set up by Scherbl et al 40 Different from our gastrointestinal model, Scherbl et al only measured the intestinal permeability of chlorogenic acids without considering the gastric step. Also in this case, 5-CQA instability and the isomerization to 3-CQA and 4-CQA were observed.…”

Section: Effect Of the In Vitro Dynamic Digestion Process At The 5-cq...supporting

confidence: 82%

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Effect of the simulated digestion process on the chlorogenic acid trapping activity against methylglyoxal

et al. 2023

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Section: Effect Of the In Vitro Dynamic Digestion Process At The 5-cq...supporting

confidence: 82%

“…32,38 More recently, the presence of an active efflux transport which leads to low intestinal absorption in a Caco-2 cell model was demonstrated. 39…”

Section: Resultsmentioning

confidence: 99%

Effect of the simulated digestion process on the chlorogenic acid trapping activity against methylglyoxal

et al. 2023

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“…To additionally validate the possibility of active L. reuteri AMBV339-derived riboflavin transport through the human intestinal epithelial cells, which would facilitate riboflavin uptake in vivo , we implemented monolayers of the Caco-2 intestinal epithelial cell line grown on ThinCerts. Differentiated Caco-2 cells recapitulate the transporting properties of the small intestinal epithelium key for riboflavin absorption ( 36 , 49 ). L. reuteri RC-14 and riboflavin at 1 mg/ml were used as control conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Testing was performed according to the protocol described in Mortelé et al ( 36 ). Briefly, Caco-2 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) with 10% foetal calf serum (FCS), non-essential amino acids and PenStrep at 37°C, 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Spontaneous Riboflavin-Overproducing Limosilactobacillus reuteri for Biofortification of Fermented Foods

et al. 2022

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Riboflavin-producing lactic acid bacteria represent a promising and cost-effective strategy for food biofortification, but production levels are typically insufficient to support daily human requirements. In this study, we describe the novel human isolate Limosilactobacillus reuteri AMBV339 as a strong food biofortification candidate. This strain shows a high natural riboflavin (vitamin B2) overproduction of 18.36 μg/ml, biomass production up to 6 × 1010 colony-forming units/ml (in the typical range of model lactobacilli), and pH-lowering capacities to a pH as low as 4.03 in common plant-based (coconut, soy, and oat) and cow milk beverages when cultured up to 72 h at 37°C. These properties were especially pronounced in coconut beverage and butter milk fermentations, and were sustained in co-culture with the model starter Streptococcus thermophilus. Furthermore, L. reuteri AMBV339 grown in laboratory media or in a coconut beverage survived in gastric juice and in a simulated gastrointestinal dialysis model with colon phase (GIDM-colon system) inoculated with fecal material from a healthy volunteer. Passive transport of L. reuteri AMBV339-produced riboflavin occurred in the small intestinal and colon stage of the GIDM system, and active transport via intestinal epithelial Caco-2 monolayers was also demonstrated. L. reuteri AMBV339 did not cause fecal microbiome perturbations in the GIDM-colon system and inhibited enteric bacterial pathogens in vitro. Taken together, our data suggests that L. reuteri AMBV339 represents a promising candidate to provide riboflavin fortification of plant-based and dairy foods, and has a high application potential in the human gastrointestinal tract.

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“…These values are corrected by a constant offset (Δlog K p = −1.12 cm/s) [ 46 ], and the final permeability values are log K p = −7.01, −8.39 and −6.69 for CAF, CHA and FA, respectively. Table 5 also shows the average calculated permeability values of CAF and FA in skin by Zhang et al [ 85 ], and experimental permeability values from Caco−2 cells for CAF, CHA and FA [ 86 , 87 ], as well as the deviation of our corrected values log K p . It can be seen that our log K p corrected values fit better to log K p (calc) of skin by Zhang et al (deviation = 0.16 and −0.46 for CAF and FA) than to the values of log K p (exp) of Caco−2 cells (deviation = 1.17, 2.79 and 1.71 for CAF, CHA and FA respectively).…”

Section: Resultsmentioning

confidence: 99%

Concentration of Antioxidant Compounds from Calendula officinalis through Sustainable Supercritical Technologies, and Computational Study of Their Permeability in Skin for Cosmetic Use

et al. 2021

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The growing interest in the cosmetic industry in using compounds of natural and sustainable origin that are safe for humans is encouraging the development of processes that can satisfy these needs. Chlorogenic acid (CHA), caffeic acid (CAF) and ferulic acid (FA) are three compounds widely used within the cosmetic industry due to their functionalities as antioxidants, collagen modifiers or even as radiation protectors. In this work, two advanced separation techniques with supercritical CO2 are used to obtain these three compounds from Calendula officinalis, and these are then evaluated using a computational skin permeability model. This model is encompassed by the COSMO-RS model, the calculations of which make it possible to study the behaviour of the compounds in the epidermis. The results show that both CAF and FA are retained in the stratum corneum, while CHA manages to penetrate to the stratum spinosum. These compounds were concentrated by antisolvent fractionation with super-critical CO2 using a Response Surface Methodology to study the effect of pressure and CO2 flow rate. CHA, CAF and FA were completely retained in the precipitation vessel, with concentrations between 40% and 70% greater than in the original extract. The conditions predicted that the optimal overall yield and enrichment achieved would be 153 bar and 42 g/min.

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Demonstrating the involvement of an active efflux mechanism in the intestinal absorption of chlorogenic acid and quinic acid using a Caco-2 bidirectional permeability assay

Abstract: The intestinal absorption of chlorogenic acid and quinic acid was investigated using an in vitro bidirectional Caco-2 permeability assay and LC-MS/MS.

Cited by 25 publications

References 35 publications

Effect of the simulated digestion process on the chlorogenic acid trapping activity against methylglyoxal

Effect of the simulated digestion process on the chlorogenic acid trapping activity against methylglyoxal

Spontaneous Riboflavin-Overproducing Limosilactobacillus reuteri for Biofortification of Fermented Foods

Concentration of Antioxidant Compounds from Calendula officinalis through Sustainable Supercritical Technologies, and Computational Study of Their Permeability in Skin for Cosmetic Use

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