Demineralized Dentin Matrix Acts as a Scaffold for Repair of Articular Cartilage Defects

Yagihashi, Keisuke; Miyazawa, Ken; Togari, Ken; Goto, Shigemi

doi:10.1007/s00223-008-9205-7

Cited by 39 publications

(40 citation statements)

References 25 publications

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“…It has also been clarified that demineralized dentin originating from various mammals induces undifferentiated mesenchymal cells to differentiate into osteogenic cells and then into cartilage and bone 23) . Although Reddi documented osteoinduction with particle sizes of 74 to 420 µm 24,25) , our previous study demonstrated particle sizes of 250 to 500 µm were highly efficient in osteoinduction 7) . In the present study, to ensure post-grafting stability, the particle size of DDM was set at 250 to 500 µm.…”

Section: Ddmmentioning

confidence: 73%

“…In order to make the particle size even, DDM with particle sizes of 250 -500 mm was collected by sieving ( Fig. 1) 7) .…”

Section: Ddm Preparationmentioning

confidence: 99%

“…Also, it has been proven that the organic matrix in dentin contains osteoinductive bone morphogenetic protein (BMP) 6) . We have demonstrated that demineralized dentin matrix (DDM) acts as a scaffold for repair of articular cartilage defects 7) . Moreover, recent studies examine a multi-component technique consisting of a novel side population of multi-purpose stem cells cultured on demineralized bone and dentin matrix, for tissue engineering applications [8][9][10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

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Bone Regeneration by Demineralized Dentin Matrix in Skull Defects of Rats

Togari

Miyazawa

Yagihashi

et al. 2012

J. Hard Tissue Biology.

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Alveolar bone defects may result in functional and morphological abnormalities. In such cases, bone can be regenerated by grafting fresh autogenous bone into the defect. Subsequently, we focused on the dentin of third molars, which are usually discarded after extraction, or teeth removed faor orthodontic therapy. As in bone, type I collagen is the main component of the organic matrix in dentin. Also, it has been proven that the organic matrix of dentin contains osteoinductive bone morphogenetic protein (BMP). It would be safe to perform grafting with the dentin of extracted teeth used as an autogenous bone substitute. Therefore, the objective of the present study was to reuse extracted teeth as a bone substitute material. Demineralized dentin matrix (DDM), an organic material derived from the dentine of bovine teeth, was grafted in rat skull defects to determine its bone regeneration ability and the possibility of its use as a bone substitute. In the present study, new bone formed from the defect margin in the control group, but in the graft group, new bone formation occurred from individual DDM granules within the defect, and not just from the margin. At Week 8, defect repair was limited in the control group. In contrast, most of the defect was covered by osseous tissue in the graft group. In conclusion, DDM may be a useful bone substitute that serves as a scaffold for bone regeneration by inducing a high level of new bone formation soon after surgery.

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Section: Ddmmentioning

confidence: 73%

“…In order to make the particle size even, DDM with particle sizes of 250 -500 mm was collected by sieving ( Fig. 1) 7) .…”

Section: Ddm Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bone Regeneration by Demineralized Dentin Matrix in Skull Defects of Rats

Togari

Miyazawa

Yagihashi

et al. 2012

J. Hard Tissue Biology.

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“…Additionally, it has also been reported that DSp is essential for growth and maintenance of mandibular condylar cartilage [42]. Although it is well known that dentin sialophosphoprotein plays an important role in the mineralization of hard tissues such as bone and teeth, demineralized dentin matrices have been shown to function as efficient scaffolds for cartilage repair [43,44]. Moreover, dentin sialophosphoprotein belongs to the family of small integrin binding ligands (SIBLING) which have several factors common with matrix extracellular phosphoglycoproteins [45].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Romanelli

Knoll

Santora

et al. 2015

Fibers

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Advances in tissue engineering have enabled the ability to design and fabricate biomaterials at the nanoscale that can actively mimic the natural cellular environment of host tissue. Of all tissues, cartilage remains difficult to regenerate due to its avascular nature. Herein we have developed two new hybrid polypeptide-glycosaminoglycan microfibrous scaffold constructs and compared their abilities to stimulate cell adhesion, proliferation, sulfated proteoglycan synthesis and soluble collagen synthesis when seeded with chondrocytes. Both constructs were designed utilizing self-assembled Fmoc-protected valyl cetylamide nanofibrous templates. The peptide components of the constructs were varied. For Construct I a short segment of dentin sialophosphoprotein followed by Type I collagen were attached to the templates using the layer-by-layer approach. For Construct II, a short peptide segment derived from the integrin subunit of Type II collagen binding protein expressed by chondrocytes was attached to the templates followed by Type II collagen. To both constructs, we then attached the natural polymer N-acetyl glucosamine, chitosan. Subsequently, the glycosaminoglycan chondroitin sulfate was then attached as the final layer. The scaffolds were characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), atomic force microscopy and scanning electron microscopy. In vitro culture studies were carried out in the presence of chondrocyte cells for OPEN ACCESSFibers 2015, 3 266 both scaffolds and growth morphology was determined through optical microscopy and scanning electron microscopy taken at different magnifications at various days of culture. Cell proliferation studies indicated that while both constructs were biocompatible and supported the growth and adhesion of chondrocytes, Construct II stimulated cell adhesion at higher rates and resulted in the formation of three dimensional cell-scaffold matrices within 24 h. Proteoglycan synthesis, a hallmark of chondrocyte cell differentiation, was also higher for Construct II compared to Construct I. Soluble collagen synthesis was also found to be higher for Construct II. The results of the above studies suggest that scaffolds designed from Construct II be superior for potential applications in cartilage tissue regeneration. The peptide components of the constructs play an important role not only in the mechanical properties in developing the scaffolds but also control cell adhesion, collagen synthesis and proteoglycan synthesis capabilities.

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“…In addition, it has been reported that BP administration near the extraction socket and extraction site and below the maxillary periosteum on the opposite side improved the osteochondral repair ability of the extraction socket [9][10][11] . However, it is unclear whether or not direct BP administration to the defective bone region exhibits a similar effect.…”

Section: Introductionmentioning

confidence: 99%