Delivery of Nucleic Acids, Proteins, and Nanoparticles by Arginine-Rich Cell-Penetrating Peptides in Rotifers

Liu, Betty Revon; Liou, Ji-Sing; Chen, Yung-Jen; Huang, Yue-Wern; Lee, Han-Jung

doi:10.1007/s10126-013-9509-0

Cited by 33 publications

(32 citation statements)

References 57 publications

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“…Estas biomoléculas emergieron como una clase especial de transportadores, que constituyen una de las herramientas más utilizadas en el transporte intracelular no invasivo de fármacos, al unirse a principios activos mediante la formación de enlaces químicos o por la formación de complejos estables no covalentes. 3 La capacidad de transporte de los CPPs, permitió la internalización celular de moléculas de distinto grado de complejidad con propiedades terapéuticas, como moléculas pequeñas, 4,5 ácidos nucleicos, 6,7 proteínas, 8 liposomas, 4,9 quantum dots 10 y agentes de contraste de resonancia magnética, 11 entre otros.…”

Section: Antecedentes Y Generalidades De Los Cppsunclassified

Péptidos que atraviesan la membrana celular como potenciales transportadores de fármacos

Pulido¹,

Royo²,

Alberício³

et al. 2016

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RESUMENEl descubrimiento y desarrollo de secuencias peptídicas (naturales o sintéticas), así como de peptidomiméticos que puedan penetrar en las células (CPPs, por sus siglas del inglés: Cell penetrating peptides), constituye un avance prometedor en la identificación de entidades capaces de promover la entrega no invasiva e íntegra de molécu-las pequeñas, ADN plasmídico, ARN de interferencia, proteínas, virus y nanopartículas, entre otras, al interior de la célula. Por lo general, estas secuencias peptídicas están compuestas de 5-30 aminoácidos y se pueden clasificar en catiónicos, anfipáticos e hidrofóbicos, teniendo en cuenta sus características estructurales. La presente revisión explora la clasificación y el estado del arte en general de esta temática, con especial énfasis en el tipo de unión entre el péptido con capacidad de atravesar la membrana celular (CPP) y la entidad a transportar.Palabras clave: Péptidos, Barrera hematoencefalica, transportadores moleculares, CPPs ABSTRACTThe discovery and development of natural, synthetic or peptidomimetic cell-penetrating peptides (CPPs) is a promising breakthrough to achieve non-invasive delivery of non-permeable biomolecules in the intracellular compartment. The CPPs are able to carry small molecules, plasmid DNA, interfering RNA, proteins, viruses, nanoparticles among others, across the cellular membrane, resulting in internalization of the intact cargos. In general, are sequence with 5-30 aminoacids, which are classified in cationics, amphipathics or hydrophobics, depending on its structural characteristics. The present review explores the classification, as well as the "state of the art" of this topic, focuses on the link between CPP and cargo.Keywords: Peptides, Molecular transportes, cell penetrating peptides, blood-brain barrie IntroducciónLas barreras biológicas son las interfaces generadas durante la evolución, que hicieron posible que los organismos abandonaran el agua y se establecieran en la tierra. Durante el proceso evolutivo, la necesidad de especificación promovió su diferenciación, con el fin de regular procesos como la homeostasis del agua, la entrada de nutrientes, el intercambio de gases o la excreción de compuestos tóxicos.1 Las barreras biológicas más estudiadas incluyen la piel, las membranas mucosas, la barrera hematoencefálica (BHE) y las barreras celulares. Estas últimas (por ejemplo, el núcleo o la mitocondria) constituyen las barreras de mayor importancia biológica si nos interesa potenciar el transporte de fármacos a través de ellas para mejorar la efectividad de los agentes terapéuticos.

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Section: Antecedentes Y Generalidades De Los Cppsunclassified

Péptidos que atraviesan la membrana celular como potenciales transportadores de fármacos

Pulido¹,

Royo²,

Alberício³

et al. 2016

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“…A synthetic R9 CPP (SR9) was used to deliver noncovalentlyassociated plasmid DNA into living plant cells [23]. This SR9 peptide, the essential component for transmembrane transport, enters plant cells where it further facilitates nuclear routing [24][25][26][27][28][29][30][31]. The negatively charged plasmid DNA bound to the positively charged SR9 peptide by electrostatic interaction.…”

Section: Cpp-mediated Dna Deliverymentioning

confidence: 99%

Cellular Delivery of Noncovalently-Associated Macromolecules by Cell- Penetrating Peptides

Chang¹,

Huang²,

Aronstam³

et al. 2014

CPB

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Cellular and nuclear delivery of biomolecules is limited by low membrane permeability. Cell-penetrating peptides (CPPs) can be covalently linked to cargos to improve cellular internalization. Our work indicates that arginine-rich CPPs are also able to interact with a variety of cargos, including DNA, RNA, proteins and nanomaterials, in a noncovalent manner and subsequently effect their delivery into cells. The advantages of noncovalent attachment in CPP-mediated transduction are multiple: ease of use, ease of production, and versatility with respect to both cargo composition and functional delivery (i.e., the cargo is not chemically modified). We have extended this approach to achieve simultaneous transduction of covalently and noncovalently associated complexes, opening a new method for delivering multiple types of cargos, including proteins, fluorescent nanomaterials, nucleic acid and others. These novel variations of CPP-mediated transport should be of broad utility in the transport of genes, small interfering RNAs, proteins and nanoparticles in biomedical research and therapeutic intervention.

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“…TAT protein is the 48-60 polypeptide fragment of the human immunodeficiency virus-1 and was the first CPP to be discovered. It has been widely used in the delivery of extracellular bioactive molecules, including small interfering RNA (28)(29)(30), nucleic acids (31,32), proteins (33,34) and QDs (35), into cells (36). Previous studies have demonstrated that the modification of the surface of QDs with CPPs improves the labeling rate of cells (37,38).…”

Section: Discussionmentioning

confidence: 99%

Effect of quantum dots on the biological behavior of the EJ human bladder urothelial carcinoma cell line

Yuan

Zhang

et al. 2015

Molecular Medicine Reports

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Abstract. Quantum dots (QDs) are a type of fluorescent label with applications in biological molecules, cells and in vivo imaging. The current study investigated the effect of QDs on the toxicity, proliferation, migration and invasion of the EJ human bladder cancer cell line in vitro. The cell counting kit-8 test was used to measure the survival rate of EJ cells following incubation with varying concentrations of QDs. Additionally, the effect of QDs on tumor cell migration and invasion was evaluated using the Transwell chamber assay, and cell proliferation rate was assessed using a hemocytometer. Data from the current study demonstrated no significant differences in survival rate between the experimental and control groups with the conventionally used concentrations (5, 10 and 20 nM) of QD605 (P>0.05). However, with high concentrations of QD605 (40 and 80 nM), significant differences were observed (P<0.001). The survival rate of EJ cells, however, remained at 92.6%. In addition, no significant differences were observed between the EJ cells labeled with transactivator of transcription (TAT)-QD605 and the unlabeled EJ cells with regard to proliferation, migration and invasion (P>0.05). Thus, the results of the current study indicate that QDs exhibit a certain degree of influence on the activity of the EJ bladder cancer cell line at high concentrations. However, at the concentrations that QDs are conventionally used, there was little impact on the survival of the EJ cells. In addition, the proliferation, migration and invasion abilities of the EJ cells were not affected by TAT-QDs. Therefore, the peptide-conjugated QDs have potential to be applied in the imaging and tracking of live cells in vitro and of animals in vivo. Notably, QDs may provide the foundation for a novel, non-invasive imaging strategy for the early diagnosis of tumors.

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Delivery of Nucleic Acids, Proteins, and Nanoparticles by Arginine-Rich Cell-Penetrating Peptides in Rotifers

Cited by 33 publications

References 57 publications

Péptidos que atraviesan la membrana celular como potenciales transportadores de fármacos

Péptidos que atraviesan la membrana celular como potenciales transportadores de fármacos

Cellular Delivery of Noncovalently-Associated Macromolecules by Cell- Penetrating Peptides

Effect of quantum dots on the biological behavior of the EJ human bladder urothelial carcinoma cell line

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