Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology

Buntru, Matthias; Gärtner, Stefanie; Staib, Lena; Kreuzaler, Fritz; Schlaich, Nikolaus Ludwig

doi:10.1007/s11248-012-9640-0

Cited by 25 publications

(17 citation statements)

References 54 publications

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“…16 More recently, 7 genes were assembled by an improved version of multiround Gateway technology. 17 Another progress for multigenes transformation came from the use of site specific recombination with homing endonucleases to stack different genes into artificial chromosome vector 18 or minichromosome. 19 Which Promoters to Use?…”

Section: Rewiring Multiple Map Kinases To Construct "Mapkmentioning

confidence: 99%

MAPK transgenic circuit to improve plant stress-tolerance?

Moustafa

2014

Plant Signaling & Behavior

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Section: Rewiring Multiple Map Kinases To Construct "Mapkmentioning

confidence: 99%

MAPK transgenic circuit to improve plant stress-tolerance?

Moustafa

2014

Plant Signaling & Behavior

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“…The resultant vector let introduce up to eight genes placed on a single destination vector, besides other sequences as selection markers, scaffold attachment regions, etc. The inserted transgenes were stably expressed for at least two generations (Buntru et al 2013 ). (c) Co-integrated vectors: they are not commonly in use because they require of long homologies between Agrobacterium and E. coli being less effi cient than binary vectors.…”

Section: Vectorsmentioning

confidence: 99%

Molecular Farming in Plants

Álvarez

2014

Plant Biotechnology for Health

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Molecular farming can generally be defi ned as the production of molecules (proteins, fatty acids) for the pharmaceutical and chemical industries in transgenic organisms (plants, animals, etc.).Molecular farming in plants has advantageous aspects as biosecurity, they do not bear pathogens for humans or animals, and they do not produce toxins. Also, plant protein synthetic machinery is able to produce complex glycosylated proteins as they have a glycosylation pattern with slight difference respect of that of mammals, and can perform foldings.When displayed in in vitro conditions, molecular farming have the characteristic advantages of that type of culture, mainly the capacity of working under Good Manufactory practices as is required by the pharmaceutical industry. The critical aspects of plants for molecular farming are the different glycosylation patterns respect to mammals, the duration of the productive process, and the relatively low yields.The approval by the FDA of the fi rst medicine to be used in humans, taliglucerase alfa (ELELYSO®), has give an impulse to this technology but there are some drawbacks to be addressed, particularly related to low yields and regulatory aspects.

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“…Initially, these vectors still suffered from the cumbersome cloning procedure due to the lack of unique restriction sites, but this issue has been taken care of by combining the vectors with Gateway site-specific recombination technology (Vega et al, 2008). Multisite and MultiRound Gateway systems have been used to integrate up to seven genes into the plant genome (Buntru et al, 2013).…”

Section: High-capacity T-dna Vectorsmentioning

confidence: 99%

Engineering metabolic pathways in plants by multigene transformation

Zorrilla-López¹,

Masip²,

Arjó³

et al. 2013

Int. J. Dev. Biol.

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Metabolic engineering in plants can be used to increase the abundance of specific valuable metabolites, but single-point interventions generally do not improve the yields of target metabolites unless that product is immediately downstream of the intervention point and there is a plentiful supply of precursors. In many cases, an intervention is necessary at an early bottleneck, sometimes the first committed step in the pathway, but is often only successful in shifting the bottleneck downstream, sometimes also causing the accumulation of an undesirable metabolic intermediate. Occasionally it has been possible to induce multiple genes in a pathway by controlling the expression of a key regulator, such as a transcription factor, but this strategy is only possible if such master regulators exist and can be identified. A more robust approach is the simultaneous expression of multiple genes in the pathway, preferably representing every critical enzymatic step, therefore removing all bottlenecks and ensuring completely unrestricted metabolic flux. This approach requires the transfer of multiple enzyme-encoding genes to the recipient plant, which is achieved most efficiently if all genes are transferred at the same time. Here we review the state of the art in multigene transformation as applied to metabolic engineering in plants, highlighting some of the most significant recent advances in the field.

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Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology

Cited by 25 publications

References 54 publications

MAPK transgenic circuit to improve plant stress-tolerance?

MAPK transgenic circuit to improve plant stress-tolerance?

Molecular Farming in Plants

Engineering metabolic pathways in plants by multigene transformation

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