Delivery of membrane impermeable molecules to primary mouse T lymphocytes

Xu, Ke; Li, Ming O.

doi:10.1016/j.xpro.2021.100757

Cited by 2 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pore-forming toxins, e.g., sticholysin [ 55 ], streptolysin O [ 56 ] and pore-forming proteins such as transmembrane proteins, cause pyroptosis, known as an inflammatory form of cell death [ 57 ]. ATP-induced pore-forming toxins (e.g., nigericin derived from Streptomyces hygroscopicus and channels such as lysenin [ 58 ] cause channelopathies [ 59 ].…”

Section: Methods Applied For Permeabilizationmentioning

confidence: 99%

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Bánfalvi

2022

IJMS

View full text Add to dashboard Cite

Eukaryotic chromosomes have not been visualized during the interphase. The fact that chromosomes cannot be seen during the interphase of the cell cycle does not mean that there are no means to make them visible. This work provides visual evidence that reversible permeabilization of the cell membrane followed by the regeneration of cell membranes allows getting a glimpse behind the nuclear curtain. Reversibly permeable eukaryotic cells have been used to synthesize nascent DNA, analyze the 5′-end of RNA primers, view individual replicons and visualize interphase chromosomes. Dextran T-150 in a slightly hypotonic permeabilizing buffer prevented cells from disruption. Upon reversal of permeabilization, the nucleus could be opened at any time during the interphase. A broad spectrum of a flexible chromatin folding pattern was revealed through a series of transient geometric forms of chromosomes. Linear attachment of chromosomes was visualized in several mammalian and lower eukaryotic cells. The linear connection of chromosomes is maintained throughout the cell cycle showing that rather than individual chromosomes, a linear array of chromosomes is the functional giant macromolecule. This study proves that not only the prokaryotic genome but also linearly attached eukaryotic chromosomes form a giant macromolecular unit.

show abstract

Section: Methods Applied For Permeabilizationmentioning

confidence: 99%

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Bánfalvi

2022

IJMS

View full text Add to dashboard Cite

show abstract

Global diversification of the common moonwort ferns (Botrychium lunaria group, Ophioglossaceae) was mainly driven by Pleistocene climatic shifts

Mossion

Koenen

Grant

et al. 2022

Preprint

View full text Add to dashboard Cite

The cosmopolitan Botrychium lunaria group belong to the most species rich genus of the family Ophioglossaceae and was considered to consist of two species until molecular studies in North America and northern Europe led to the recognition of multiple new taxa. Recently, additional genetic lineages were found scattered in Europe, emphasizing our poor understanding of the global diversity of the B. lunaria group, while the processes involved in the diversification of the group remain unexplored. We conducted the first global phylogenetic study of the group including 513 ingroup accessions sequenced for four non-coding plastid loci. We recovered ten well-supported clades, although relationships between clades were inconsistent between Bayesian and Maximum Likelihood analyses. We treated each clade at the species level, except for one clade including two ploidy levels, resulting in the recognition of 11 species, 5 of which are unnamed. In contrast to previous studies, we found species diversity to be equally distributed across the northern hemisphere, with 7-8 species per continent. We estimated the stem age of the B. lunaria group at 2.4-5.1 million years, with most species 1.8-2.6 million years old, and subspecies 0.5-1.0 million years old. Diversification thus coincided with Pleistocene climatic fluctuations that strongly affected the areas inhabited by the group, suggesting that diversification was driven by climatically induced cycles of extinction, dispersal, and migration. Furthermore, ecological differentiation between species suggests these complex population dynamics were associated with adaptations to specific environmental conditions. We found limited evidence that speciation is driven by polyploidization and hybridization. We show that the B. lunaria group has greater species level diversity than previously assumed and suspect that further cryptic species may await discovery, especially in the B. neolunaria clade.

show abstract

Delivery of membrane impermeable molecules to primary mouse T lymphocytes

Cited by 2 publications

References 2 publications

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Global diversification of the common moonwort ferns (Botrychium lunaria group, Ophioglossaceae) was mainly driven by Pleistocene climatic shifts

Contact Info

Product

Resources

About