Delivery of macromolecules in unstimulated T cells by photoporation with polydopamine nanoparticles

Berdecka, Dominika; Harizaj, Aranit; Goemaere, Ilia; Punj, Deep; Goetgeluk, Glenn; Munter, Stijn De; Keersmaecker, Herlinde De; Boterberg, Veerle; Dubruel, Peter; Vandekerckhove, Bart; Smedt, Stefaan C. De; Vos, Winnok H. De; Braeckmans, Kevin

doi:10.1016/j.jconrel.2023.01.047

Cited by 7 publications

(5 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a follow-up study, we investigated the delivery of 500 kDa FITC-dextran in nonactivated human T cells, finding that smaller BSA@PD NPs of 150 or 250 nm have less impact on T-cell functionality than 400 nm particles. 49 These findings show that photoporation with BSA@PD NPs can be used to deliver compounds in quiescent T cells as well but that the nanoparticles should be small enough to avoid damaging cells too much. Recently, we also reported on the use of a response surface methodology to facilitate finding the optimal parameters for the photoporation of cells with BSA@PD NPs (size, concentration, and laser fluence).…”

Section: Biodegradable Nanoparticlesmentioning

confidence: 88%

“…This means that the overall yield of living transfected T cells was ∼20%, being 2.5-fold larger than what could be achieved with electroporation as a benchmark technology. In a follow-up study, we investigated the delivery of 500 kDa FITC-dextran in nonactivated human T cells, finding that smaller BSA@PD NPs of 150 or 250 nm have less impact on T-cell functionality than 400 nm particles . These findings show that photoporation with BSA@PD NPs can be used to deliver compounds in quiescent T cells as well but that the nanoparticles should be small enough to avoid damaging cells too much.…”

Section: Single Photothermal Nanostructuresmentioning

confidence: 99%

See 1 more Smart Citation

Photothermal Nanomaterial-Mediated Photoporation

et al. 2023

Self Cite

View full text Add to dashboard Cite

Figure 3. Intracellular delivery of effector molecules in various cell types by AuNP-mediated photoporation. (a) AuNP-mediated photoporation of H1299 eGFP cells with varying laser intensity for siRNA gene silencing. eGFP expression was quantified with flow cytometry after 24 h. (b) eGFP-mRNA transfection in HeLa cells by AuNP-mediated photoporation with 0.3 μM eGFP-mRNA using increasing laser fluence and a fixed AuNP concentration of 8 × 10 7 NPs/mL. (c) H1299-eGFP knockout levels after photoporation in the presence of decreasing concentrations of eGFP RNPs. (d) Cell viability and gene silencing in cytotoxic T lymphocytes (CTLs) by AuNP-mediated photoporation versus electroporation (nucleofection). (e) Labeling primary neurons by incubation with the lipophilic dye DiI leads to staining artifacts in microscopy images, such as the presence of debris and uneven staining across the cells. Instead, the intracellular delivery of phalloidin by photoporation resulted in high quality staining of selected neuronal cells. (f) AuNP-mediated photoporation of macrophages with FITC-dextran of 150 kDa at a laser fluence of 1.6 J/cm 2 for increasing AuNP concentration.

show abstract

Section: Biodegradable Nanoparticlesmentioning

confidence: 88%

Section: Single Photothermal Nanostructuresmentioning

confidence: 99%

Photothermal Nanomaterial-Mediated Photoporation

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…[167][168][169] To overcome this problem, PDA NPs were developed and successfully used for photoporation because they are biocompatible and biodegradable. 170,171 However, further studies are required to validate their in vivo degradation process, which is yet not fully understood. 172 For more elaborate information regarding these PSs and their applications for photoporation, we refer readers to a recent review article published by Xiong et al 23 In this context, single small molecules can be promising due to their small molecular structure making their degradation easier for the body.…”

Section: Photoablation Of Biological Aggregates and Barriers Using Dyesmentioning

confidence: 99%

“…It has been displayed that fragmented gold NPs can potentially intercalate with DNA and induce genotoxicity 167–169 . To overcome this problem, PDA NPs were developed and successfully used for photoporation because they are biocompatible and biodegradable 170,171 . However, further studies are required to validate their in vivo degradation process, which is yet not fully understood 172 .…”

Section: Biomedical Applications Of Dyesmentioning

confidence: 99%

Supramolecular dye nanoassemblies for advanced diagnostics and therapies

Ramezani,

De Smedt,

Sauvage

2024

Bioengineering & Transla Med

Self Cite

View full text Add to dashboard Cite

Dyes have conventionally been used in medicine for staining cells, tissues, and organelles. Since these compounds are also known as photosensitizers (PSs) which exhibit photoresponsivity upon photon illumination, there is a high desire towards formulating these molecules into nanoparticles (NPs) to achieve improved delivery efficiency and enhanced stability for novel imaging and therapeutic applications. Furthermore, it has been shown that some of the photophysical properties of these molecules can be altered upon NP formation thereby playing a major role in the outcome of their application. In this review, we primarily focus on introducing dye categories, their formulation strategies and how these strategies affect their photophysical properties in the context of photothermal and non‐photothermal applications. More specifically, the most recent progress showing the potential of dye supramolecular assemblies in modalities such as photoacoustic and fluorescence imaging, photothermal and photodynamic therapies as well as their employment in photoablation as a novel modality will be outlined. Aside from their photophysical activity, we delve shortly into the emerging application of dyes as drug stabilizing agents where these molecules are used together with aggregator molecules to form stable nanoparticles.

show abstract

“…[32][33][34] In recent years, researchers have discovered that PDA has an excellent photothermal property, i.e., the ability to generate heat in the presence of light. 35,36 This special property makes PDA an ideal material for photothermal conversion. These properties give PDA a wide range of application prospects in the elds of photothermal therapy, photothermal conversion and photothermal catalysis.…”

Section: Introductionmentioning

confidence: 99%