Delivery of DNAzyme targeting c-Myc gene using β-cyclodextrin polymer nanocarrier for therapeutic application in human breast cancer cell line

Farrokhi, Fatemeh; Karami, Zahra; Esmaeili‐Mahani, Saeed; Heydari, Abolfazl

doi:10.1016/j.jddst.2018.08.015

Cited by 21 publications

(13 citation statements)

References 51 publications

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“…These vectors use CD as the main constituent of the gene delivery system, as compared to the previously explored works which integrate CD to perform a secondary role. Examples include cyclodextrin-based nanosponges [105], nanoparticles [84,106,107], vesicles [108], and hydrogels [11]. Typically, these formulations are composed of CD derivatives.…”

Section: Pure CD Vectorsmentioning

confidence: 99%

“…Gene delivery can be achieved through the usage of carboxymethyl-β-cyclodextrin, a simple modification which is commercially available [110]. Some groups have had success delivering DNAzymes, which form 1:1 polyplexes with CD and can target and suppress genes of interest [107,111]. Other groups have utilized the host/guest ability of CD to create responsive drug delivery systems, which function through binding competition or more effective isomerization, where the weaker complex CD forms with genetic material is an advantage, as it can be undone by a molecule with a stronger affinity [112].…”

Section: Pure CD Vectorsmentioning

confidence: 99%

“…In the first category, a pure beta-CD nanoparticle was fabricated to hold DNAzyme, which did little to kill cancer cells (10-20% cell death) on its own, but was found to work synergistically with DOX to improve effects [107]. In this work, DOX was not loaded into the nanoparticle itself, but was instead administered in a soluble form which mimics systemic administration.…”

Section: Future Combination Therapy Applicationsmentioning

confidence: 99%

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Cyclodextrins in drug delivery: applications in gene and combination therapy

Haley

Gottardi

Langer

et al. 2020

Drug Deliv. and Transl. Res.

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Gene therapy is a powerful tool against genetic disorders and cancer, targeting the source of the disease rather than just treating the symptoms. While much of the initial success of gene delivery relied on viral vectors, non-viral vectors are emerging as promising gene delivery systems for efficacious treatment with decreased toxicity concerns. However, the delivery of genetic material is still challenging, and there is a need for vectors which show improved targeting, further reduced toxicity, and controlled release. In this article, we highlight current work in gene therapy which utilizes the cyclic oligosaccharide molecule cyclodextrin (CD). With a number of unique abilities, such as hosting small molecule drugs, acting as a linker or modular component, reducing immunogenicity, and disrupting membranes, CD is a valuable constituent in many delivery systems. Further, it shows great promise in combination therapies due to the ease of assembling macromolecular structures and wide variety of chemical derivatives, which allow for customizable delivery systems and simultaneous co-delivery of therapeutics. The use of combination and personalized therapies can result in improved patient health -modular systems, such as those which incorporate CD, are more conducive to these therapy types.

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Section: Pure CD Vectorsmentioning

confidence: 99%

Section: Pure CD Vectorsmentioning

confidence: 99%

Section: Future Combination Therapy Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Cyclodextrins in drug delivery: applications in gene and combination therapy

Haley

Gottardi

Langer

et al. 2020

Drug Deliv. and Transl. Res.

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“…A growing list of NPs and their conjugates have been developed for healthcare applications, for example iron oxide NPs (IONPs), AuNPs, QDs, carbon nanotubes (CNTs), PBE functionalized graphene oxide, and Fe 3 O 4 /poly(D,L-lactide) (PDLLA) nanocomposites [93][94][95][96]. The advantageous properties of DNAzymes favor their conjugation to nanocarriers for high-efficacy therapeutics [97], and several DNAzymes have been explored in the treatment of different types of cancer (Table 3).…”

Section: Dnazymes As Therapeutic Agentsmentioning

confidence: 99%

Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications

Kumar

Jain

Dilbaghi

et al. 2019

Trends in Biochemical Sciences

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DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research. Glossary Chromatography: a technique used for the physical separation of mixtures into their components. All forms of chromatography work on the same principle, in that, all have a stationary phase and a mobile phase. The approach is wellknown for its high sensitivity because of competitive interaction between the desired molecule and target immobilized stationary phase that are mainly composed of agarose beads. Combinatorial chemistry: synthetic method used to prepare a library of structurally related analogues in a single process. Currently, vast ranges of simulation software are used for generating combinatorial library. Electrochemical detection: the basic principle of electrochemical biosensors involves immobilization of selected functional DNA molecules on an electrode surface that specifically bind to a ligand, which can further lead to a change in the current or voltage. Electrophoresis: analysis technique used to efficiently separate charged particles from each another based on differences in their mobility. It can be used to separate a variety of small and large target molecules. The major shortcoming associated is that the target molecule should have a sufficient size to induce mobility changes. G-quadruplex DNAzyme: single-stranded G-rich aptamer sequences spontaneously form an intramolecular four-stranded helical structure (G4). Associates with the hemin group to form the catalytic G4-hemin-DNAzyme complex. This complex DNAzyme catalyzes the oxidation reaction of hydrogen peroxide to result in the generation of chemiluminescence. Logic gate: a logic gate performs a logical operation (Boolean algebra) on given inputs in order to generate a single electrical output. Different logic gates are combined using combinatorial or sequential approaches for computer programming. Basic logic gate series include-AND, OR, NOT, XOR, NOR, NAND, and XNOR.

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“…The first key issue is how to efficiently deliver DNAzymes to cancer cells. DNAzymes have to overcome multiple physiological barriers during transportation from the administration site to cancer tissues, including high interstitial pressure of the tumor tissue, the abundant tumor extracelllular matrix (ECM), and high risk of being degraded by nucleases (Farrokhi et al 2018;Xiao et al 2019). Therefore, it is necessary to explore stable vectors to improve the efficiency of DNAzymes for overcoming these obstacles.…”

Section: Introductionmentioning

confidence: 99%

Recent advances of DNAzyme-based nanotherapeutic platform in cancer gene therapy

Huo

Wang

et al. 2020

Biophys Rep

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Deoxyribozyme (or denoted as DNAzyme), which is produced by in vitro screening technology, has gained extensive research interest in the field of biomedicine due to its high catalytic activity and structure identification. This review introduces the structural characteristics of RNA-cleaving DNAzyme and its application potential in cancer gene therapy, which plays a significant role in cancer-related gene inactivation by specifically cleaving target mRNA and inhibiting the expression of the corresponding protein. However, the low delivery efficiency and cellular uptake hindered the widespread usage of DNAzyme in gene therapy of cancers. Emerging nanotechnology holds great promise for DNAzyme to overcome these obstacles. This review mainly focuses on DNAzyme-based nanotherapeutic platforms in gene therapy of cancers, including oncogene antagonism therapy, treatment resistance gene therapy, immunogene therapy, and antiangiogenesis gene therapy. We also revealed the potential of DNAzyme-based nanotherapeutic platforms as emerging cancer therapy approaches and their security issues.

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Delivery of DNAzyme targeting c-Myc gene using β-cyclodextrin polymer nanocarrier for therapeutic application in human breast cancer cell line

Cited by 21 publications

References 51 publications

Cyclodextrins in drug delivery: applications in gene and combination therapy

Cyclodextrins in drug delivery: applications in gene and combination therapy

Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications

Recent advances of DNAzyme-based nanotherapeutic platform in cancer gene therapy

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