Delivering DNA Origami to Cells

Balakrishnan, Dhanasekaran; Wilkens, Gerrit David; Heddle, Jonathan G.

doi:10.2217/nnm-2018-0440

Cited by 41 publications

(35 citation statements)

References 83 publications

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“…DNA nanotechnology has facilitated the design of a library of nanostructures that have been shown to be stable in cellular environments and can be readily modified with small molecules or protein ligands to study cellular signaling at the nanoscale or act as programmable delivery vehicles. 54,55 Here, we evaluated receptor binding efficiency of antibody-functionalized DNA nanostructures to elucidate critical design parameters that can promote or hamper cellular binding. Our results reveal that, while the native affinity of incorporated antibodies remains unaltered, the absolute number of surface receptors targeted by antibody-functionalized DNA nanostructures is reduced compared to free antibodies.…”

Section: Discussionmentioning

confidence: 99%

Determinants of ligand-functionalized DNA nanostructure-cell interactions

Cremers

Rosier

Meijs

et al. 2021

Preprint

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Synthesis of ligand-functionalized nanomaterials with control over size, shape and ligand orientation, facilitates the design of tailored nanomedicines for therapeutic purposes. DNA nanotechnology has emerged as a powerful tool to rationally construct two- and three-dimensional nanostructures, enabling site-specific incorporation of protein ligands with control over stoichiometry and orientation. To efficiently target cell surface receptors, exploration of the parameters that modulate cellular accessibility of these nanostructures is essential. In this study we systematically investigate tunable design parameters of antibody-functionalized DNA nanostructures binding to therapeutically relevant receptors. We show that, although the native affinity of antibody-functionalized DNA nanostructures remains unaltered, the absolute number of bound surface receptors is lower compared to soluble antibodies and is mainly governed by nanostructure size and DNA handle location. The obtained results provide key insights in the ability of ligand-functionalized DNA nanostructures to bind surface receptors and yields design rules for optimal cellular targeting.

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Section: Discussionmentioning

confidence: 99%

Determinants of ligand-functionalized DNA nanostructure-cell interactions

Cremers

Rosier

Meijs

et al. 2021

Preprint

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“…The influence of rod-like or disk-like nano-particles on the orientational order parameter of the LCLC is investigated in the low concentration limit, where interaction of the nano-particles can be neglected. The DNA origami technique [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ] was used in order to synthesize nano-particles with extremely precise shape. In addition to the baseline investigation presented here, LC–NP-composites have potential for complex developments, because the highly precise tailored NPs obtained by the DNA origami technique may be ordered on a larger scale through LC–NP interaction, thereby bridging the gap between NP synthesis and NP assembly, and finally controlling macroscopic material properties.…”

Section: Introductionmentioning

confidence: 99%

“…This level of control facilitates fabrication with near-atomic precision [ 27 , 28 ]. Functionalization with chromophores, semiconducting, plasmonic or magnetic particles, and photo- or biosensitive molecular units, tensegrity designs, incorporation of molecular springs or single-stranded DNA handles, and other modifications have not only facilitated the fabrication of very precise static and dynamic nanostructures for microscopic fundamental research, but also made applications in photonics, materials science, biology and medicine feasible [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

DNA Origami Nano-Sheets and Nano-Rods Alter the Orientational Order in a Lyotropic Chromonic Liquid Crystal

et al. 2020

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Rod-like and sheet-like nano-particles made of desoxyribonucleic acid (DNA) fabricated by the DNA origami method (base sequence-controlled self-organized folding of DNA) are dispersed in a lyotropic chromonic liquid crystal made of an aqueous solution of disodium cromoglycate. The respective liquid crystalline nanodispersions are doped with a dichroic fluorescent dye and their orientational order parameter is studied by means of polarized fluorescence spectroscopy. The presence of the nano-particles is found to slightly reduce the orientational order parameter of the nematic mesophase. Nano-rods with a large length/width ratio tend to preserve the orientational order, while more compact stiff nano-rods and especially nano-sheets reduce the order parameter to a larger extent. In spite of the difference between the sizes of the DNA nano-particles and the rod-like columnar aggregates forming the liquid crystal, a similarity between the shapes of the former and the latter seems to be better compatible with the orientational order of the liquid crystal.

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“…For instance, due to its excellent cell-friendly properties such as the robustness of nanotubes and high cell permeability, dna or dna structures are increasing intracellular permeability with the help of nanotubes [105]. Or DNA nanostructures that simulate the characteristics of nanotubes are designed and replaced [106]. Recent advances in these carbon nanotube-DNA complexes have begun to realize this potential but is still at the earliest stage and a number of hurdles remain.…”

Section: Carbon Nanotube-dna Complexmentioning

confidence: 99%

Hybrid material of structural DNA with inorganic compound: synthesis, applications, and perspective

et al. 2020

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Owing to its precise manipulation in nanoscale, DNA as a genetic code becomes a promising and generic material in lots of nanotechnological outstanding exploitations. The nanoscale assembly of nucleic acids in aqueous solution has showed very remarkable capability that is not achievable from any other material resources. In the meantime, their striking role played by effective intracellular interactions have been identified, making these more attractive for a variety of biological applications. Lately, a number of interesting attempts have been made to augment their marvelous diagnostic and therapeutic capabilities, as being integrated with inorganic compounds involving gold, iron oxide, quantum dot, upconversion, etc. It was profoundly studied how structural DNA-inorganic hybrid materials have complemented with each other in a synergistic way for better-graded biological performances. Such hybrid materials consisting of both structural DNAs and inorganics are gradually receiving much attention as a practical and futureoriented material substitute. However, any special review articles highlighting the significant and innovative materials have yet to be published. At the first time, we here demonstrate novel hybrid complexes made of structural DNAs and inorganics for some practical applications.

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Delivering DNA Origami to Cells

Cited by 41 publications

References 83 publications

Determinants of ligand-functionalized DNA nanostructure-cell interactions

Determinants of ligand-functionalized DNA nanostructure-cell interactions

DNA Origami Nano-Sheets and Nano-Rods Alter the Orientational Order in a Lyotropic Chromonic Liquid Crystal

Hybrid material of structural DNA with inorganic compound: synthesis, applications, and perspective

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