Delivered adipose‐derived stromal cells improve host‐derived adipose tissue formation in composite constructs in vivo

Abstract

NA. Laryngoscope, 127:E428-E436, 2017.

Uncultivated stromal vascular fraction is equivalent to adipose‐derived stem and stromal cells on porous polyurethrane scaffolds forming adipose tissue in vivo

“…Therefore, almost all implanted cells must have been degraded to the time point of histological evaluation. Taken together, we assume that the formation of adipose tissue and the neovascularization is more likely attributable to mediators, secreted by seeded ASCs and SVF cells . These agents might, for example, be IGF, VEGF, bFGF, and HGF …”

“…In particular, in the outer areas, significantly more host cells were attracted to migrate into the constructs. This effect was most likely attributable to soluble factors secreted by ASCs, as unseeded controls, both noninduced and induced, merely exhibited poor tissue formation . Blood vessel formation was insignificantly reduced by adipogenic induction but might have been beneficial at earlier time points of tissue formation, as the authors suggest …”

“…Informed consent was given by every participant. The tissue was processed as previously described by our group, and detailed protocols can be found in the Supporting Information in the online version of this article …”

“…ASCs have been widely used in adipose tissue engineering research for the last few years, and they are being applied in clinical practice in other specialties . A long‐term prevailing challenge in adipose tissue engineering, namely ASC differentiation to major adipocytes and simultaneous vascularization, has lately been put into context by Storck et al, who have shown that adipogenically induced ASCs do not undergo differentiation themselves; instead, they act as modulators for host cells to invade scaffolds and form coherent well‐vascularized fat pads . This influence of incorporated cells on the host response has also been reported for the SVF …”

“…[10][11][12][13][14] These stem cells, isolated from the SVF of adipose tissue, the so-called ASCs, 15 were first detected by Zuk et al and showed potential to differentiate into adipogenic, chondrogenic, osteogenic, myogenic, or neuronal cells. 16,17 ASCs have been widely used in adipose tissue engineering research for the last few years, [18][19][20] and they are being applied in clinical practice in other specialties. 21 A long-term prevailing challenge in adipose tissue engineering, namely ASC differentiation to major adipocytes and simultaneous vascularization, has lately been put into context by Storck et al, who have shown that adipogenically induced ASCs do not undergo differentiation themselves; instead, they act as modulators for host cells to invade scaffolds and form coherent wellvascularized fat pads.…”