Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species

Acinetobacter silvestris sp. nov. discovered in forest ecosystems in Czechia

“…Core genome-based phylogenetic analysis was carried out as described previously [5]and included the annotation of draft genome sequences using Prokka [15], obtaining groups of single copy orthologs presented in all genomes with OrthoFinder [16], aligning the coding sequences of orthologs using mafft [17] and phylogenetic inference based on the concatenation and trimming of the alignments followed by maximum-likelihood tree reconstruction on the resulting supermatrix with RAxML 8.1.9 using the GTR-GAMMA model [18]. shows the core genome-based phylogram of these sequences within the context of the genomes of the type strains of all known Acinetobacter species with correct, validly published names.…”

“…Full-length 16S rRNA gene sequences were extracted from the whole genome sequences of the Taxon 35 strains and compared with those of the type strains of the known Acinetobacter species with correct, validly published names using the Bionumerics 7.6 software (Applied-Maths) as described previously [5]. The analysis was conducted on the region corresponding to the 1542-nt complete sequence of the 16S rRNA gene of Escherichia coli K-12 (DDBJ/ENA/GenBank accession no.…”

“…Acinetobacter is a taxonomically diverse and ecologically ubiquitous eubacterial genus, which has a deep evolutionary history [2,3]. To understand the taxonomic structure of the genus in soil and aquatic ecosystems which are not directly associated with human activities, we have conducted a long-term prospective study to assess the diversity at the species level of strains recovered from protected landscape areas in Czechia [4,5]. The study resulted in the description of six species [4][5][6][7][8] and several taxonomically unique groups of strains (apps.szu.cz/anemec/Classification.pdf) or single strains (L. Radolfová-Křížová and A.…”

“…To understand the taxonomic structure of the genus in soil and aquatic ecosystems which are not directly associated with human activities, we have conducted a long-term prospective study to assess the diversity at the species level of strains recovered from protected landscape areas in Czechia [4, 5]. The study resulted in the description of six species [4–8] and several taxonomically unique groups of strains (apps.szu.cz/anemec/Classification.pdf) or single strains (L. Radolfová-Křížová and A. Nemec, unpublished), which are likely to represent additional novel species. The aim of the present study was to define the taxonomic status of one of the novel unique groups, which was provisionally designated Taxon 35 and included five strains collected from different localities in protected forest areas.…”

“…Metabolic and physiological properties of Acinetobacter silvestris sp. nov. (Taxon 35) and the other species described among soil and water isolates from Czechia The results for species other than A. silvestris were published previously[4][5][6][7][8]. Assimilation tests were interpreted after 6 days of culture at 25 °C (A. silvestris and A. celticus) or 30 °C (the other species).…”