High poly ε-caprolactone biodegradation activity by a new Acinetobacter seifertii isolate

Budkum, Jirawan; Thammasittirong, Sutticha Na-Ranong; Thammasittirong, Anon

doi:10.1007/s12223-022-00964-7

Cited by 5 publications

(2 citation statements)

References 46 publications

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“…Due to the slow degradation of PCL in vivo , several in vitro models have been established to investigate its degradation mechanisms. , The in vitro degradation studies of PCL are often conducted in PBS with a pH 7.4. Because of the slow degradation rate of PCL in PBS at pH 7.4, numerous researchers have focused on the impact of enzymes on PCL degradation. , Various enzymes play a crucial role in polymer biodegradation, and often employed to accelerate degradation in in vitro studies of PCL. It has been reported in previous studies that several kinds of lipases, especially lipase-type enzymes, such as Rhizopus delemer lipase, Rhizopus arrhizuslipase, Pseudomonas, , and lipase of porcine pancreas, were effective to accelerate the degradation rate of PCL to induce nonuniform surface erosion.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Vascular Grafts Using Poly(ε-Caprolactone) and Collagen-Encapsuled ADSCs for Interposition Implantation of Abdominal Aorta in Rhesus Monkeys

Jiang,

Zuo,

Wang

et al. 2024

ACS Biomater. Sci. Eng.

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The production of small-diameter artificial vascular grafts continues to encounter numerous challenges, with concerns regarding the degradation rate and endothelialization being particularly critical. In this study, porous PCL scaffolds were prepared, and PCL vascular grafts were fabricated by 3D bioprinting of collagen materials containing adipose-derived mesenchymal stem cells (ADSCs) on the internal wall of the porous PCL scaffold. The PCL vascular grafts were then implanted in the abdominal aorta of Rhesus monkeys for up to 640 days to analyze the degradation of the scaffolds and regeneration of the aorta. Changes in surface morphology, mechanical properties, crystallization property, and molecular weight of porous PCL revealed a similar degradation process of PCL in PBS at pH 7.4 containing Thermomyces lanuginosus lipase and in situ in the abdominal aorta of rhesus monkeys. The contrast of in vitro and in vivo degradation provided valuable reference data for predicting in vivo degradation based on in vitro enzymatic degradation of PCL for further optimization of PCL vascular graft fabrication. Histological analysis through hematoxylin and eosin (HE) staining and fluorescence immunostaining demonstrated that the PCL vascular grafts successfully induced vascular regeneration in the abdominal aorta over the 640-day period. These findings provided valuable insights into the regeneration processes of the implanted vascular grafts. Overall, this study highlights the significant potential of PCL vascular grafts for the regeneration of small-diameter blood vessels.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Vascular Grafts Using Poly(ε-Caprolactone) and Collagen-Encapsuled ADSCs for Interposition Implantation of Abdominal Aorta in Rhesus Monkeys

Jiang,

Zuo,

Wang

et al. 2024

ACS Biomater. Sci. Eng.

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“…Multiple studies have reported both aerobic and anaerobic PCL-degrading bacteria or fungi, including Acinetobacter spp., Alcaligenes faecalis, Bacillus spp., Brevundimonas sp., Clostridium sp., Lactobacillus spp., Pseudomonas spp., Streptomyces spp., Aspergillus spp., Fusarium spp., Mucor spp., Paecilomyces lilacinus, Penicillium spp., and Rhizopus spp. (Murphy et al, 1996 ; Tiago et al, 2004 ; Tokiwa et al, 2009 ; Nawaz et al, 2014 ; Khan et al, 2017 ; Bartnikowski et al, 2019 ; Mandic et al, 2019 ; Suzuki et al, 2021 ; Budkum et al, 2022 ; Atanasova et al, 2023 ). The degradation of PCL is carried out by esterases and/or depolymerases secreted extracellularly.…”

Section: Introductionmentioning

confidence: 99%

Insights into the biodegradation of polycaprolactone through genomic analysis of two plastic-degrading Rhodococcus bacteria

Zampolli,

Vezzini,

Brocca

et al. 2024

Front. Microbiol.

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Polycaprolactone (PCL) is an aliphatic polyester often utilized as a model to investigate the biodegradation potential of bacteria and the involved catabolic enzymes. This study aims to characterize PCL biodegradative metabolic potential and correlate it to genomic traits of two plastic-degrading bacteria—Rhodococcus erythropolis D4 strain, a new isolate from plastic-rich organic waste treatment plant, and Rhodococcus opacus R7, known for its relevant biodegradative potential on polyethylene and similar compounds. After preliminary screening for bacteria capable of hydrolyzing tributyrin and PCL, the biodegradation of PCL was evaluated in R. erythropolis D4 and R. opacus R7 by measuring their growth and the release of PCL catabolism products up to 42 days. After 7 days, an increase of at least one order of magnitude of cell number was observed. GC-MS analyses of 28-day culture supernatants showed an increase in carboxylic acids in both Rhodococcus cultures. Furthermore, hydrolytic activity (~5 U mg−1) on short/medium-chain p-nitrophenyl esters was detected in their supernatant. Finally, a comparative genome analysis was performed between two Rhodococcus strains. A comparison with genes annotated in reference strains revealed hundreds of gene products putatively related to polyester biodegradation. Based on additional predictive analysis of gene products, gene expression was performed on a smaller group of genes, revealing that exposure to PCL elicits the greatest increase in transcription for a single gene in strain R7 and two genes, including that encoding a putative lipase, in strain D4. This work exhibits a multifaceted experimental approach to exploit the broad potential of Rhodococcus strains in the field of plastic biodegradation.

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Microorganisms that produce enzymes active on biodegradable polyesters are ubiquitous

et al. 2023

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High poly ε-caprolactone biodegradation activity by a new Acinetobacter seifertii isolate

Cited by 5 publications

References 46 publications

Fabrication of Vascular Grafts Using Poly(ε-Caprolactone) and Collagen-Encapsuled ADSCs for Interposition Implantation of Abdominal Aorta in Rhesus Monkeys

Fabrication of Vascular Grafts Using Poly(ε-Caprolactone) and Collagen-Encapsuled ADSCs for Interposition Implantation of Abdominal Aorta in Rhesus Monkeys

Insights into the biodegradation of polycaprolactone through genomic analysis of two plastic-degrading Rhodococcus bacteria

Microorganisms that produce enzymes active on biodegradable polyesters are ubiquitous

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