Delimitation of the time of death by immunohistochemical detection of thyroglobulin

Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry?

“…Wehner et al [60][61][62] studied if a positive immunoreaction to various antigens like insulin, thyroglobulin or calcitonin is correlated with the time since death ( Table 6). The philosophy of these investigations is that with increasing postmortem interval the tertiary structure of the antigen undergoes postmortal changes and due to protein denaturation staining becomes negative.…”

Histochemical detection of platelet esterase activity in the bone marrow postmortem: can megakaryocytes serve as indicators for time since death?

“…Earlier studies on the delimitation of the time since death by immunohistochemical staining of inner organs detecting, for example, insulin,9 thyroglobulin,10 glucagon,11 and calcitonin12 in forensic corpses stated that it was possible to define a maximum time since death in case of a negative reaction. The authors described a time-dependent loss of stainability within the different immunoreactions which was influenced by external factors, such as cause of death or surrounding conditions of the individual case.…”

Post-mortem analysis of bone marrow osteoclasts using tartrate-resistant acid phosphatase staining: does histochemistry work and correlate with time since death?

“…Earlier studies on the delimitation of the time since death by immunohistochemical staining of inner organs detecting, for example, insulin,11 thyroglobulin,12 glucagon13 and calcitonin14 in forensic corpses stated that it was possible to at least define a maximum time since death in the case of a negative reaction. In these studies, the authors described a time-dependent loss of stainability within the different immunoreactions which was also influenced by various external factors such as cause of death or surrounding conditions of the individual case.…”