Post-mortem analysis of bone marrow osteoclasts using tartrate-resistant acid phosphatase staining: does histochemistry work and correlate with time since death?

Boehm, Joachim; Schmidt, Ulrike; Porsche, Michéle; Veeck, Jürgen; Schaefer, Hans-Eckart

doi:10.1136/jclinpath-2012-200854

Cited by 9 publications

(6 citation statements)

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“…The samples used to estimate the PMI came from different organs, such as brain [ 38 , 39 , 42 ], thyroid [ 35 , 36 ], pancreas [ 34 , 37 , 38 ], gingival tissue [ 14 , 27 ], skin [ 44 ], heart [ 33 , 43 ], large intestine [ 45 ], female genital system [ 46 ], bone marrow [ 40 ], skeletal muscle [ 41 ], as well as lung and liver [ 42 ]. For these tissues, the various authors used different markers, without any overlap or repetition ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…Further work on 74 subjects was conducted by Boehm et al [ 40 ], who evaluated the chronological variation of the expression of the antigenic marker Tartrate-resistant acid phosphatase (TRAP) in bone marrow. They reported that TRAP was detectable in osteoclasts with a constant intensity until the seventh day after death, after which there was a sudden and total negativity of the immunoreaction.…”

Section: Resultsmentioning

confidence: 99%

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New Trends in Immunohistochemical Methods to Estimate the Time since Death: A Review

et al. 2022

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The identification of a reliable and accurate post-mortem interval (PMI) is a major challenge in the field of forensic sciences and criminal investigation. Several laboratory techniques have recently been developed that offer a better contribution to the estimation of PMI, in addition to the traditional physical or physico-chemical (body cooling, lividity, radiocarbon dating, rigor mortis), chemical (autolysis), microbiological (putrefaction), entomological, as well as botanical parameters. Molecular biology (degradation pattern of macromolecules such as proteins, DNA, RNA), biochemical analysis of biological fluids (such as blood, cerebrospinal fluid, and vitreous humor), and immunohistochemistry are some of the most recent technological innovations. A systematic review of the literature was performed with the aim of presenting an up-to-date overview on the correlation between the immunohistochemical (IHC) expression of specific antigenic markers at different PMIs. The systematic review was performed according to PRISMA guidelines. Scopus and PubMed were used as search engines from January 1, 1998 to March 1, 2022 to evaluate the effectiveness of immunohistochemistry in estimating PMI. The following keywords were used: (immunohistochemical) OR (immunohistochemistry) AND (time since death) OR (post-mortem interval) OR (PMI). A total of 6571 articles were collected. Ultimately, 16 studies were included in this review. The results of this systematic review highlighted that IHC techniques, in association with traditional methods, add, in Bayesian terms, additional information to define a more accurate time of death and PMI. However, current IHC results are numerically limited and more data and studies are desirable in the near future.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

New Trends in Immunohistochemical Methods to Estimate the Time since Death: A Review

et al. 2022

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“…After µCT scanning, specimens were decalcified in an EDTA solution, dehydrated in ethanol (70%-100%), and embedded in paraffin. Sections (~5 μm) were cut transversely and stained with tartrate-resistant acid phosphatase (TRAP) stain and osteopontin (OPN) stain as established biomarkers for osteoclast and osteoblast activity, respectively (Colnot et al, 2007;Boehm et al, 2012;Bondarenko et al, 2013). Images were acquired by optical light microscopy (Carl Zeiss Micro Imaging GmbH, Göttingen, Germany).…”

Section: Paraffin Embedding and Histologymentioning

confidence: 99%

Calcium-phosphate-coated Oral Implants Promote Osseointegration in Osteoporosis

et al. 2013

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Osteoporotic conditions are anticipated to affect the osseointegration of dental implants. This study aimed to evaluate the effect of a radiofrequent magnetron-sputtered calcium phosphate (CaP) coating on dental implant integration upon installment in the femoral condyles of both healthy and osteoporotic rats. At 8 weeks post-implantation, bone volume and histomorphometric bone area were lower around non-coated implants in osteoporotic rats compared with healthy rats. Interestingly, push-out tests revealed significantly enhanced implant fixation for CaP-coated compared with non-coated implants in both osteoporotic (i.e., 2.9-fold) and healthy rats (i.e., 1.5-fold), with similar implant fixation for CaP-coated implants in osteoporotic conditions compared with that of non-coated implants in healthy conditions. Further, the presence of a CaP coating significantly increased bone-to-implant contact compared with that in non-coated implants in both osteoporotic (i.e., 1.3-fold) and healthy rats (i.e., 1.4-fold). Sequential administration of fluorochrome labels showed significantly increased bone dynamics close to CaP-coated implants at 3 weeks of implantation in osteoporotic conditions and significantly decreased bone dynamics in osteoporotic compared with healthy conditions. In conclusion, analysis of the data obtained demonstrated that dental implant modification with a thin CaP coating effectively improves osseointegration in both healthy and osteoporotic conditions.

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“…Recently, we have reported that tartrate-resistant acid phosphatase (TRAP) staining of autopsy samples of FFPE bone marrow (BM) was feasible in general, but TRAP staining intensities (SI) were not associated with postmortem intervals (PMI) 1. α-Naphthyl acetate esterase (ANAE) is a further enzyme of only few known to date that are detectable on FFPE BM samples.…”

Section: Introductionmentioning

confidence: 99%

Histochemical detection of platelet esterase activity in the bone marrow postmortem: can megakaryocytes serve as indicators for time since death?

et al. 2013

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Post-mortem analysis of bone marrow osteoclasts using tartrate-resistant acid phosphatase staining: does histochemistry work and correlate with time since death?

Cited by 9 publications

References 8 publications

New Trends in Immunohistochemical Methods to Estimate the Time since Death: A Review

New Trends in Immunohistochemical Methods to Estimate the Time since Death: A Review

Calcium-phosphate-coated Oral Implants Promote Osseointegration in Osteoporosis

Histochemical detection of platelet esterase activity in the bone marrow postmortem: can megakaryocytes serve as indicators for time since death?

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