Delignification of the cotton stalk and ginning mill waste via EnZolv pretreatment and optimization of process parameters using response surface methodology (RSM)

Subramaniam, Santhoshkumar; Kumutha, K.; ASM, Raja; Uthandi, Sivakumar

doi:10.1016/j.biortech.2023.129655

Cited by 7 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results gained are in agreement with earlier research done by Iniyakumar and his colleagues [ 12 ]. Optimization of enzyme load in EnZolv pretreatment of CSMW showed lignin reduction in the range of 21.27%–32.40% which was in agreement with previous study using CS and GMW as biomass [ 31 ]. A buffer-free solvent medium limits the activity of enzyme in changing pH environments, and limits solubility of proteins in solvents.…”

Section: Discussionsupporting

confidence: 90%

“…In the present study, conventional EnZolv pretreatment of five different CSMW showed a lignin reduction in the range of 35–61%. The results are in agreement with lignin reduction of 45.36% and 35.02% using EnZolv pretreatment of cotton stalk (CS) and ginning mill waste (GMW), respectively [ 31 ]. A concurrent study using CSMW for pretreatment with Pleurotus florida exhibited lignin degradation of 60% (w/w) [ 32 ].…”

Section: Discussionsupporting

confidence: 72%

“…Incubation time optimization in CSMW due to EnZolv showed maximum lignin reduction at 5 h (CN), 10 h (FS, LF, and MD), 15 h (BD). This was evidenced by the previous findings which showed EnZolv pretreatment with maximum lignin removal at 5 h incubation time for both CS and GMW [ 31 ]. Incubation temperature of 40 °C (LF), and 45 °C (BD, FS, MD, CN) showed maximum lignin reduction due to EnZolv pretreatment which was in accordance with CS and GMW biomass that exhibited maximum removal at 50 °C and 45 °C, respectively [ 31 ].…”

Section: Discussionsupporting

confidence: 71%

“…This was evidenced by the previous findings which showed EnZolv pretreatment with maximum lignin removal at 5 h incubation time for both CS and GMW [ 31 ]. Incubation temperature of 40 °C (LF), and 45 °C (BD, FS, MD, CN) showed maximum lignin reduction due to EnZolv pretreatment which was in accordance with CS and GMW biomass that exhibited maximum removal at 50 °C and 45 °C, respectively [ 31 ]. Previous investigations using EnZolv pretreatment showed maximum lignin reduction at 150 rpm and 80 rpm for CS and GMW biomass, respectively [ 31 ].…”

Section: Discussionsupporting

confidence: 71%

“…CSMW reported maximum lignin reduction due to moisture content optimization in EnZolv pretreatment of 11.81%, and 80.70% at 0% moisture content for BD, and LF, 60.30% at 4% moisture content for MD, and 65.22%, and 76.72% at 100% moisture content for FS, and CN, respectively. A similar study for optimizing moisture content in EnZolv pretreatment of CS and GMW revealed maximum delignification of 69.3% and 71.52% at 0% and 100% moisture content, respectively [ 31 ]. The results gained are in agreement with earlier research done by Iniyakumar and his colleagues [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM)

Subramaniam,

Karunanandham,

Raja

et al. 2024

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

Background EnZolv is a novel enzyme-based, eco-friendly biomass pretreatment process that has shown great potential in the field of textile engineering and biotechnology. It employs laccase from Hexagonia hirta MSF2 and 2% ethanol in the process of delignification. The process is designed to evaluate optimal conditions to remove lignin and other impurities from cotton spinning mill waste (CSMW), without compromising the quality and strength of the fibers. CSMW is a low-cost and readily available source of cellulose, making it an ideal candidate for delignification using EnZolv. By optimizing the pretreatment conditions and harnessing the potential of enzymatic delignification, this research aims to contribute to more sustainable and efficient ways of utilizing lignocellulosic biomass in various industries for the production of biochemical and bioproducts. Results The present study emphasizes the EnZolv pretreatment in the delignification of cotton spinning mill wastes irrespective of the cellulose content. EnZolv process parameters such as, moisture content, enzyme load, incubation time, incubation temperature, and shaking speed were optimized. Under pre-optimized conditions, the percent lignin reduction was 61.34%, 61.64%, 41.85%, 35.34%, and 35.83% in blowroom droppings (BD), flat strips (FS), lickerin fly (LF), microdust (MD) and comber noils (CN), respectively. Using response surface methodology (RSM), the statistically optimized EnZolv pretreatment conditions showed lignin reduction of 59.16%, 62.88%, 48.26%, 34.64%, and 45.99% in BD, FS, LF, MD, and CN, respectively. Conclusion Traditional chemical-based pretreatment methods often involve harsh chemicals and high energy consumption, which can have detrimental effects on the environment. In contrast, EnZolv offers a greener approach by utilizing enzymes that are biodegradable and more environmentally friendly. The resulting fibers from EnZolv treatment exhibit improved properties that make them suitable for various applications. Some of the key properties include enhanced cellulose recovery, reduced lignin content, and improved biophysical and structural characteristics. These improvements can contribute to the fiber's performance and processability in different industries and future thrust for the production of cellulose-derived and lignin-derived bioproducts. Graphical Abstract

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 72%

Section: Discussionsupporting

confidence: 71%

Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM)

Subramaniam,

Karunanandham,

Raja

et al. 2024

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

show abstract

Extrusion puffing as a pretreatment method to change the surface structure, physicochemical properties and in vitro protein digestibility of distillers dried grains with solubles

Liu,

Xie,

Deng

et al. 2023

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDDistillers dried grains with solubles (DDGS) are rich in nutrition, and they are potential protein feed raw material. However, the existence of cellulose, hemicellulose and lignin hinders animals' digestion and absorption of DDGS. Making full use of unconventional feed resources such as DDGS can alleviate the shortage of feed resources to a certain extent. This research investigated the effects of twin‐screw extrusion on the macromolecular composition, physical and chemical properties, surface structure and in vitro protein digestibility (IVPD) of DDGS.RESULTSThe findings showed that extrusion puffing significantly increased the protein solubility, bulk density, water holding capacity, and swelling capacity, while significantly decreased hemicellulose and crude protein content, particle size and zeta potential of DDGS. The structure damage of DDGS induced by the extrusion was characterized by scanning electron microscopy (SEM), Fourier‐transform infrared (FITR) spectroscopy and X‐ray diffraction (XRD) analysis. Interestingly, no random coil was observed in the analysis of the secondary structure, and extrusion promoted the transformation of α‐helix and β‐turn to β‐sheet, which led to significant increases in protein solubility and IVPD of DDGS (P < 0.05). Additionally, correlation analysis revealed that IVPD and PS had a positive relationship.CONCLUSIONExtrusion puffing was an ideal pretreatment method for DDGS modification to improve in vitro protein digestibility. © 2023 Society of Chemical Industry.

show abstract

Biomass pretreatment, bioprocessing and reactor design for biohydrogen production: a review

Sahil,

Singh,

Masakapalli

et al. 2024

Environ Chem Lett

View full text Add to dashboard Cite

Delignification of the cotton stalk and ginning mill waste via EnZolv pretreatment and optimization of process parameters using response surface methodology (RSM)

Cited by 7 publications

References 35 publications

EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM)

EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM)

Extrusion puffing as a pretreatment method to change the surface structure, physicochemical properties and in vitro protein digestibility of distillers dried grains with solubles

Biomass pretreatment, bioprocessing and reactor design for biohydrogen production: a review

Contact Info

Product

Resources

About