Deletion of sua gene reduces the ability of Streptococcus uberis to adhere to and internalize into bovine mammary epithelial cells

Chen, Xueyan; Dego, Oudessa Kerro; Almeida, Ricardo Augusto Monteiro de Barros; Fuller, Troy E.; Luther, Douglas A.; Oliver, S. P.

doi:10.1016/j.vetmic.2010.07.006

Cited by 18 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same group demonstrated that bovine serum and milk containing anti-SUAM or anti-pepSUAM antibodies significantly inhibited adherence to and internalization of S. uberis into BMEC . This observation was further confirmed by a study using a sua-gene deletion mutant strain of S. uberis UT888 (Chen et al, 2011). Using 3 different prediction methods, the N-terminal pepSUAM (also designated as SUAM peptide I), 3 other regions in the middle portion of SUAM [peptide II (AA 342-356), peptide III (AA 357-371), and peptide IV (AA 439-453], and a region at the C-terminus of SUAM [peptide V (AA 705-719)] were predicted to be surfaced exposed B-cell epitopes Luther et al, 2010).…”

mentioning

confidence: 55%

Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of Streptococcus uberis isolated from geographically diverse areas

Yuan

Dego

Chen

et al. 2014

Journal of Dairy Science

Self Cite

View full text Add to dashboard Cite

The objective was to identify and sequence the sua gene (GenBank no. DQ232760; http://www.ncbi.nlm.nih.gov/genbank/) and detect Streptococcus uberis adhesion molecule (SUAM) expression by Western blot using serum from naturally S. uberis-infected cows in strains of S. uberis isolated in milk from cows with mastitis from geographically diverse areas of the world. All strains evaluated yielded a 4.4-kb sua-containing PCR fragment that was subsequently sequenced. Deduced SUAM AA sequences from those S. uberis strains evaluated shared >97% identity. The pepSUAM sequence located at the N terminus of SUAM was >99% identical among strains of S. uberis. Streptococcus uberis adhesion molecule expression was detected in all strains of S. uberis tested. These results suggest that sua is ubiquitous among strains of S. uberis isolated from diverse geographic locations and that SUAM is immunogenic.

show abstract

mentioning

confidence: 55%

Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of Streptococcus uberis isolated from geographically diverse areas

Yuan

Dego

Chen

et al. 2014

Journal of Dairy Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Invasion, persistence, and internalization are up regulated by binding of SUAM to lactoferrin, which is primarily synthesized in those cells (ALMEIDA et al, 2006;CHEN et al, 2011;LUTHER et al, 2008). This gene is reported to be highly conserved (LUTHER et al, 2008;PERRIG et al, 2015), supporting its potential utility for the development of vaccines.…”

Section: Resultsmentioning

confidence: 97%

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Loures¹,

Pereira²,

Carvalho-Castro³

et al. 2017

SCA

View full text Add to dashboard Cite

ResumoA mastite é uma das doenças infecciosas mais onerosas em bovinos leiteiros em todos os continentes. Trata-se de uma doença de cunho multifatoral causada por diferentes microrganismos, incluindo vírus, leveduras, algas, parasitas e várias espécies de bactérias. Entre as bactérias, Streptococcus uberis destacase como um importante agente ambiental, responsável por uma grande variedade de infecções clínicas e subclínicas da glândula mamária, especialmente em sistemas de produção intensivos. Apesar da crescente importância de S. uberis na etiologia da mastite bovina, existem poucos estudos sobre a diversidade populacional e fatores de virulência deste patógeno em rebanhos leiteiros brasileiros.Os objetivos do presente estudo foram investigar as características de virulência e a epidemiologia molecular de S. uberis isolados de mastite bovina em rebanhos brasileiros, utilizando-se a PCR e a eletroforese em gel de campo pulsado (PFGE). Para tal, 46 amostras de S. uberis isoladas de mastite bovina em 26 rebanhos leiteiros de uma mesma mesorregião brasileira foram avaliadas quanto à diversidade populacional por eletroforese em gel de campo pulsado (PFGE), utilizando-se a enzima Smal. Além disso, foi realizada PCR para detectar os genes de virulência pauA e skc, que codificam ativadores de plasminogênio, e do gene sua, que codifica uma proteína de adesão às celulas epiteliais da glândula mamária. Nossos resultados mostraram uma alta diversidade genética na população estudada, com vários padrões de PFGE, e uma elevada frequência dos genes de virulência pesquisados: sua (100%), pauA (91%) e skc (91%). A alta frequência dos genes skc, pauA e sua sugere a importância destes fatores de virulência para S. uberis na colonização da glândula mamária bovina. Estudos moleculares e genéticos sobre este agente podem contribuir muito para melhorar o conhecimento sobre a patogenia, permitindo identificar moléculas que tenham papel relevante no estabelecimento da infecção, fornecendo informações importantes para a adoção de medidas mais eficazes para a prevenção e o controle da mastite bovina. AbstractMastitis is one of the most common and costly infectious diseases in dairy cattle worldwide. This is a multifactorial illness caused by different microorganisms, including virus, yeasts, algae, parasites, and several species of bacteria. Among these bacteria, Streptococcus uberis is an important environmental pathogen that is responsible for a large range of clinical and subclinical mammary infections, especially in intensively managed herds. Despite the increasing importance of this pathogen in the etiology of bovine mastitis, data on its virulence and diversity in Brazilian dairy herds are scarce. The aims of the present study were to investigate the virulence characteristics of S. uberis isolated from bovine mastitis and to assess the molecular epidemiology of the Brazilian isolates using pulsed-field gel electrophoresis (PFGE). In this work, 46 strains of S. uberis isolated from bovine mastitis from 26 Brazilian dairy herds were ...

show abstract

“…Streptococcus uberis UT888, originally isolated from a clinical case of mastitis, and ∆sua S. uberis UT888 were used (Chen et al, 2011). For challenge, S. uberis strains were plated onto trypticase soy blood agar plates (BAP, Becton Dickinson and Company, Franklin Lakes, NJ, USA), and incubated overnight at 37°C in 5% CO 2.…”

Section: Bacteria and Culture Conditionsmentioning

confidence: 99%

“…In following studies, we created a sua-gene deletion mutant clone of S. uberis UT888 unable to express SUAM showing reduced adherence to and internalization of the SUAM mutant into BMEC (Chen et al, 2011). However, although all these data suggested the virulence attributes of SUAM the confirmation of the pathogenic role of SUAM under in vivo conditions was still missing.…”

Section: Intramammary Infectionsmentioning

confidence: 99%

“…Subsequently, we reported a novel bacterial protein referred as Streptococcus uberis Adhesion Molecule (SUAM) that has affinity for lactoferrin (Almeida et al, 2006). Using a Sua deletion mutant (Chen, 2011) we showed that SUAM has central role on adherence to and internalization of S. uberis into bovine mammary epithelial cells (BMEC). Hence, the aim of the present study was to define the role of SUAM by comparing the outcomes of experimental intramammary infections caused by the wild type S. uberis UT888 and its isogenic ∆sua S. uberis UT888.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role of Streptococcus uberis adhesion molecule in the pathogenesis of Streptococcus uberis mastitis

Almeida

Dego

Headrick

et al. 2015

Veterinary Microbiology

Self Cite

View full text Add to dashboard Cite

Deletion of sua gene reduces the ability of Streptococcus uberis to adhere to and internalize into bovine mammary epithelial cells

Cited by 18 publications

References 30 publications

Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of Streptococcus uberis isolated from geographically diverse areas

Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of Streptococcus uberis isolated from geographically diverse areas

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Role of Streptococcus uberis adhesion molecule in the pathogenesis of Streptococcus uberis mastitis

Contact Info

Product

Resources

About