Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of Streptococcus uberis isolated from geographically diverse areas

Abstract

The objective was to identify and sequence the sua gene (GenBank no. DQ232760; http://www.ncbi.nlm.nih.gov/genbank/) and detect Streptococcus uberis adhesion molecule (SUAM) expression by Western blot using serum from naturally S. uberis-infected cows in strains of S. uberis isolated in milk from cows with mastitis from geographically diverse areas of the world. All strains evaluated yielded a 4.4-kb sua-containing PCR fragment that was subsequently sequenced. Deduced SUAM AA sequences from those S. uberis strains evaluated shared >97% identity. The pepSUAM sequence located at the N terminus of SUAM was >99% identical among strains of S. uberis. Streptococcus uberis adhesion molecule expression was detected in all strains of S. uberis tested. These results suggest that sua is ubiquitous among strains of S. uberis isolated from diverse geographic locations and that SUAM is immunogenic.

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

“…PCR analysis detected the sua gene in 100% (n = 45) of the strains, which was the most common gene found in the screened isolates. The high frequency of the sua gene in this study was similar to that related by other researchers (LUTHER et al, 2008;PERRIG et al, 2015;YUAN et al, 2014) in strains of S. uberis isolated from geographically diverse locations. However, our results were different from previous studies that showed slightly lower frequencies for the sua gene: 83.3% in Argentina (REINOSO et al, 2011) and84.6% in India (SHOME et al, 2012).…”

Genotyping and study of the pauA and sua genes of Streptococcus uberis isolates from bovine mastitis

“…The study of amino acid sequences showed several repeated mutations in different isolates. Yuan et al described similar results in sua gene conservation among isolates from different countries 46 . The relevance of the present findings, compared with previous studies, relies on the higher number (n = 137), the strict criteria used for the selection and broad distribution of the isolates analyzed.…”

“…Correspondingly, the presence of the sua gene was detected in 97.8 % of the 137 isolates. Previous works reported a prevalence of the sua gene of 83.3 % 33 in Argentina, 84.6 % in India 36 and 100 % in New Zealand, United States and England 23,46 . In addition, the nucleotide and amino acid sequences from the 20 selected S. uberis mastitis isolates had an identity greater than 97 % with respect to the reference sequence (ABB52003.1).…”

“…The analysis of the 137 isolates revealed 61 types of PFGE patterns, 29 of which were unique isolates, and 32 types, containing 2---8 isolates per type. Isolate distribution in groups was: 19 types with 2 isolates (PF 04, 9,11,12,15,16,18,21,23,25,27,37,43,45,46,47,55, 56 and 57), 5 types with 3 isolates (PF 13, 19, 32, 33 and 40), 5 types with 4 isolates (PF 20, 31, 34, 35 and 39), 2 types with 6 isolates (PF14 and PF29) and 1 type with 8 isolates (PF1) ( Table 1). Genotype grouping appeared to be neither related to the mastitis type (clinical or subclinical) nor to the farm origin.…”

<b>Capacidade de formação de biofilme e resistência aos antimicrobianos de <i>Staphylococcus aureus </i>e <i>Streptococcus uberis</i> causadores de mastite bovina</b>

“…No presente estudo, não foi utilizada a adição de leite na avaliação da formação de biofilme e mesmo assim à maioria dos isolados formaram biofilme, o que sugere que outros mecanismos pode estar envolvidos na formação de biofilme por S. uberis. Foi descrito que S. uberis possui uma molécula de adesão e internalização chamada SUAM, que está presente em isolados de diversas localizações geográficas (Yuan et al, 2014). A aderência e internalização é o mecanismo central da patogênese do S. uberis em infecções intramamárias (Almeida et al, Entretanto, devido ao número limitado de estudos envolvendo a formação de biofilme pelo S. uberis, sugere-se que estudos adicionais sejam feitos para elucidar os fatores envolvidos na formação de biofilme, os quais ainda não são conhecidos nessa espécie.…”