Deleting ku80 improves the efficiency of targeted gene editing in Neospora caninum

Redirecting DNA repair for efficient CRISPR-Cas-based gene targeting in tomato

“…The Ku70/80 complex was shown to play roles in early sensing DSBs, binding and activating the cNHEJ pathway (Symington and Gautier, 2011). Knocking out or blocking KU70/80 complex by chemicals led to the enhancement of the alternative pathways, including the HR (Chu et al, 2015; Nishizawa-Yokoi et al, 2012; Schmidt et al, 2019; West et al, 2002; Wu et al, 2022). A truncated Ku80(449–732) fragment showed a dominant-negative activity that interferes with the NHEJ pathway in hamster (Marangoni et al, 2000).…”

“…Secondly, the frequency of HR is heightened by incorporating a viral replicon vehicle for donor templates (Baltes et al, 2014; Cermak et al, 2015; Hummel et al, 2018; Vu et al, 2020). Likewise, several novel approaches have been reported in supporting elevated GT efficiency in mammals and plants, including the facilitating the HR pathway choice via suppression of the canonical nonhomologous end-joining (cNHEJ) by chemical (Chu et al, 2015; Vu et al, 2021a) or biological agents (Endo et al, 2016; Movahedi et al, 2022; Nishizawa-Yokoi et al, 2012; Qi et al, 2013; Wu et al, 2022) and facilitation of DSB end resection by introducing DSB end processing enzymes (Charpentier et al, 2018; Park et al, 2021).…”