Redirecting DNA repair for efficient CRISPR-Cas-based gene targeting in tomato
Tien Van Vu,
Ngan Thi Nguyen,
Jihae Kim
et al.
Abstract:The CRISPR-Cas-based gene targeting (GT) method has enabled precise modifications of genomic DNA ranging from single base to several kilobase scales through homologous recombination (HR). In plant somatic cells, canonical nonhomologous end-joining (cNHEJ) is the predominant mechanism for repairing double-stranded breaks (DSBs), thus limiting the HR-mediated GT. In this study, we implemented various approaches to shift the repair pathway preference toward HR by using a dominant-negative KU80 mutant protein (KUD… Show more
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