Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino &amp; E.C. Oliveira (Gracilariales, Rhodophyta)

Ursi, Suzana; Guimarães, Melina; Plastino, Estela M.

doi:10.1590/s0102-33062008000300024

Cited by 20 publications

(12 citation statements)

References 23 publications

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“…They were transported to the laboratory and unialgal cultures were established from fragments, as described by Oliveira et al (1995). Cultures were maintained in Provasoli's enriched seawater (20 mL L −1 ; 31 psu salinity), which was prepared without Tris phosphate (Ursi et al 2008), in a temperaturecontrolled room at 9±1°C and 55 μmol photons m −2 s −1 PAR provided by Philips TLT 20 W/54 daylight fluorescent tubes, on a 12 h light/12 h dark cycle. The medium was renewed weekly.…”

Section: Methodsmentioning

confidence: 99%

Iridaea cordata (Gigartinales, Rhodophyta): responses to artificial UVB radiation

2009

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The effects of UVB radiation on the different developmental stages of the carrageenan-producing red alga Iridaea cordata were evaluated considering: (1) carpospore and discoid germling mortality; (2) growth rates and morphology of young tetrasporophytes; and (3) growth rates and pigment content of field-collected plant fragments. Unialgal cultures were submitted to 0.17, 0.5, or 0.83 Wm −2 of UVB radiation for 3 h per day. The general culture conditions were as follows: 12 h light/12 h dark cycles; irradiance of 55µmol photon . per square meter per second; temperature of 9±1°C; and seawater enriched with Provasoli solution. All UVB irradiation treatments were harmful to carpospores (0:17 W m À2 ¼ 40:9 AE 6:9%, 0:5 W m À2 ¼ 59:8 AE 13:4%, 0:83 W m À2 ¼ 49 AE 17:4% mortality in 3 days). Even though the mortality of all discoid germlings exposed to UVB radiation was unchanged when compared to the control, those germlings exposed to 0.5 and 0.83 Wm −2 treatments became paler and had smaller diameters than those cultivated under control treatment. Decreases in growth rates were observed in young tetrasporophytes, mainly in 0.5 and 0.83 Wm −2 treatments. Similar effects were only observed in fragments of adult plants cultivated at 0.83 Wm −2 . Additionally, UVB radiation caused morphological changes in fragments of adult plants in the first week, while the young individuals only displayed this pattern during the third week. The verified morphological alterations in I. cordata could be interpreted as a defense against UVB by reducing the area exposed to radiation. However, a high level of radiation appears to produce irreparable damage, especially under longterm exposure. Our results suggest that the sensitivity to ultraviolet radiation decreases with increased algal age and that the various developmental stages have different responses when exposed to the same doses of UVB radiation.

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Section: Methodsmentioning

confidence: 99%

Iridaea cordata (Gigartinales, Rhodophyta): responses to artificial UVB radiation

2009

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“…Compared to wildtype, the light-green strain showed lower growth rates (Plastino et al 2004). The best culture conditions in the laboratory for growth and reproduction were provided (Ursi and Plastino 2001;Ursi et al 2008), making it possible to complete the life history of wild-type, greenish-brown, and green strains of G. birdiae in a laboratory culture . The photosynthetic, respiratory, and carotenoid concentrations were also investigated for these strains (Ursi et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Color inheritance and pigment characterization of red (wild-type), greenish-brown, and green strains of Gracilaria birdiae (Gracilariales, Rhodophyta)

Costa

Plastino

2011

J Appl Phycol

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Species of Gracilaria are some of the most useful algae in the world for the production of agar. As a consequence of its economic importance, the genus has been the subject of many studies worldwide. Color variants of Gracilaria birdiae have been found in the natural population on the Brazilian coast, and they have also been isolated from plants cultivated in laboratory. These findings raised new questions regarding intraspecific variation and the prospects of cultivating such variants for their agar production. Therefore, this work aimed to determine the mode of color inheritance for two G. birdiae strains: a greenish-brown strain (gb) found in a natural population and a green strain (gr) which had arisen as a spontaneous mutation in a red plant cultured in the laboratory. The pigment contents of these strains, as well as the red wildtype (rd), were also characterized. Crosses between female and male plants of the same color (rd, gr, or gb) and between different colors were performed. Crosses between plants of the same color showed tetrasporophytic and gametophytic descendents of the parental color. Recessive nuclear inheritance was found in the greenish-brown strain, and cytoplasmic maternal inheritance was found in the green strain; both had lower phycoerythrin and higher concentrations of allophycocyanin and phycocyanin than the wild-type. Chlorophyll a contents were similar among all strains. Taken together, our results contribute to knowledge about the variability of this important red algae. In addition, since greenish-brown and green strains showed stability of color, both could be selected and tested in experimental sea cultivation to evaluate if mutants have advantageous performance when compared with red strain.

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“…This species has recently been the focus of several laboratory studies (Plastino, 2004) and its life history has been completed in a laboratory culture (Costa & Plastino, 2001). Strain selection and the physiological demands of the strains were performed with the aim of improving cultivation techniques in the sea (Costa & Plastino, 2011;Ursi & Plastino, 2001;Ursi et al, 2003;Plastino et al, 2004;Ursi et al, 2008). Studies related to the cultivation of this species have been conducted in different environments such as shrimp ponds (Marinho-Soriano et al, 2009;Oliveira, 2007), estuaries (Marinho-Soriano et al, 2006) and the sea (Bezerra & Marinho-Soriano, 2010), showing promising growth rates.…”

Section: Introductionmentioning

confidence: 99%

Aspectos ecológicos de Gracilaria Grev. (Gracilariales, Rhodophyta) em uma praia tropical brasileira

Lyra¹,

Nunes²,

Jesus³

et al. 2011

RGCI

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Abstract:The reproductive phenology and thallus length of Gracilaria birdiae were studied over a period of 12 months in a natural bed in Northeastern Brazil. Fertile specimens of G. birdiae were observed during the entire study period. Tetrasporophytes were the most common with an annual mean of 80.1±5.6%, followed by cystocarpic plants (9.3±3.4%), male gametophytes (8.3±3.6%) and infertile plants (2.2±3.4%). Only male gametophytes and infertile plants showed a variation in occurrence frequency during the year (p<0.05). With respect to thallus length, a distinct seasonal variation was observed for all reproductive stages (p<0.05), with the highest values recorded during the rainy season (March to August) and the lowest in the dry season (September to February). The results demonstrate that the size of individuals in this population is significantly affected by the periodic changes in the environment caused by rainfall regimes and hydrodynamism.

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Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta)

Cited by 20 publications

References 23 publications

Iridaea cordata (Gigartinales, Rhodophyta): responses to artificial UVB radiation

Iridaea cordata (Gigartinales, Rhodophyta): responses to artificial UVB radiation

Color inheritance and pigment characterization of red (wild-type), greenish-brown, and green strains of Gracilaria birdiae (Gracilariales, Rhodophyta)

Aspectos ecológicos de Gracilaria Grev. (Gracilariales, Rhodophyta) em uma praia tropical brasileira

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