Deleterious effect of chronic continuous hypoxia on oral health

Terrizzi, Antonela Romina; Fernández‐Solari, Javier; Lee, Ching M.; Conti, Maria; Martínez, María Pilar

doi:10.1016/j.archoralbio.2016.08.006

Cited by 10 publications

(6 citation statements)

References 25 publications

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“…This is related to the fact that adult animals ate the same amount of food in control and experimental conditions, allowing them to grow and develop similarly. Previously reported studies from our laboratory had shown that the development of growing rats was affected by hypoxia [17, 21, 30], the reason why this study was planned with adult animals.…”

Section: Discussionmentioning

confidence: 99%

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The Process of Acclimation to Chronic Hypoxia Leads to Submandibular Gland and Periodontal Alterations: An Insight on the Role of Inflammatory Mediators

Terrizzi

Conti

Martínez

et al. 2018

Mediators of Inflammation

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The exposition to hypoxia is a stressful stimulus, and the organism develops acclimation mechanisms to ensure homeostasis, but if this fails, it leads to the development of pathological processes. Considering the large number of people under hypoxic conditions, it is of utmost importance to study the mechanisms implicated in hypoxic acclimation in oral tissues and the possible alteration of some important inflammatory markers that regulate salivary and periodontal function. It is the aim of the present study to analyze submandibular (SMG) and periodontal status of animals chronically exposed to continuous (CCH) or intermittent (CIH) hypoxia in order to elucidate the underlying molecular mechanisms that may lead to hypoxic acclimation. Adult Wistar rats were exposed to CCH or CIH simulating 4200 meters of altitude during 90 days. Salivary secretion was decreased in animals exposed to hypoxia, being lower in CIH, together with increased prostaglandin E2 (PGE2) content, TBARS concentration, and the presence of apoptotic nuclei and irregular secretion granules in SMG. AQP-5 mRNA levels decreased in both hypoxic groups. Only the CCH group showed higher HIF-1α staining, while CIH alone exhibited interradicular bone loss and increased concentration of the bone resorption marker CTX-I. In summary, animals exposed to CIH show a worse salivary secretion rate, which related with higher levels of PGE2, suggesting a negative role of this inflammatory mediator during hypoxia acclimation. We link the weak immunorreactivity of HIF-1α in CIH with improper hypoxia acclimation, which is necessary to sustaining SMG physiology under this environmental condition. The alveolar bone loss observed in CIH rats could be due mainly to a direct effect of PGE2, as suggested by its higher content in gingival tissue, but also to the indirect effect of hyposalivation. This study may eventually contribute to finding therapeutics to treat the decreased salivary flow, improving in that way oral health.

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Section: Discussionmentioning

confidence: 99%

“…Interradicular bone volume was measured as bone volume (BV)/total volume (TV) (%). TV was taken as bone tissue plus bone narrow and periodontal ligament [30]. Histopathological analyzed of the gum tissue around the first molar was also performed.…”

Section: Methodsmentioning

confidence: 99%

The Process of Acclimation to Chronic Hypoxia Leads to Submandibular Gland and Periodontal Alterations: An Insight on the Role of Inflammatory Mediators

Terrizzi

Conti

Martínez

et al. 2018

Mediators of Inflammation

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“…In this study, the number of cells seeded was standardized to an ideal size for which the cells in the core of the uninfected MT remained healthy and unaffected by hypoxia. However, a modification of the protocol of MT synthesis may be done, through an increase in the number of the cells seeded, to evaluate the effect of hypoxia on cells response to infection as this phenomenon was demonstrated to be deleterious to oral health 40 . Such 3D model may also be interesting in the future to evaluate the effects of anti-infectious or anti-inflammatory drugs while considering tissular penetration.…”

Section: Discussionmentioning

confidence: 99%

Porphyromonas gingivalis bypasses epithelial barrier and modulates fibroblastic inflammatory response in an in vitro 3D spheroid model

Bugueno

Batool

Keller

et al. 2018

Sci Rep

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Porphyromonas gingivalis-induced inflammatory effects are mostly investigated in monolayer cultured cells. The aim of this study was to develop a 3D spheroid model of gingiva to take into account epithelio-fibroblastic interactions. Human gingival epithelial cells (ECs) and human oral fibroblasts (FBs) were cultured by hanging drop method to generate 3D microtissue (MT) whose structure was analyzed on histological sections and the cell-to-cell interactions were observed by scanning and transmission electron microscopy (SEM and TEM). MTs were infected by P. gingivalis and the impact on cell death (Apaf-1, caspase-3), inflammatory markers (TNF-α, IL-6, IL-8) and extracellular matrix components (Col-IV, E-cadherin, integrin β1) was evaluated by immunohistochemistry and RT-qPCR. Results were compared to those observed in situ in experimental periodontitis and in human gingival biopsies. MTs exhibited a well-defined spatial organization where ECs were organized in an external cellular multilayer, while, FBs constituted the core. The infection of MT demonstrated the ability of P. gingivalis to bypass the epithelial barrier in order to reach the fibroblastic core and induce disorganization of the spheroid structure. An increased cell death was observed in fibroblastic core. The development of such 3D model may be useful to define the role of EC–FB interactions on periodontal host-immune response and to assess the efficacy of new therapeutics.

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“…Periodontitis is one of the most prevalent inflammatory diseases of the oral cavity and, when not properly treated, it may lead to the destruction of periodontium and tooth loss as well as to other systemic alterations, including metabolic disorders or cardiovascular alterations. − Periodontal disease is induced mainly by opportunistic anaerobic bacteria whose proliferation is favored by the characteristic low partial pressure of oxygen (pO 2 ) in the periodontal pocket as well as by the hypoxia environment elicited by inflammatory responses. − The insufficient oxygen availability also restricts the proper regeneration of the lost periodontal tissues once it affects cell survival, growth, and differentiation. − In this sense, the control over the local pO 2 can stimulate tissue regeneration and help mitigate infections into the periodontal defect site.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications

Santos

Dias

Surur

et al. 2022

ACS Appl. Nano Mater.

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Oxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2 NPs) and manganese dioxide nanosheets (MnO2 NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2 NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2 NSs were applied as a nanozyme to catalyze the decomposition of H2O2 to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2 NPs and MnO2 NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2 NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2 NP and MnO2 NS were incorporated into the fiber’s structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration.

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Deleterious effect of chronic continuous hypoxia on oral health

Cited by 10 publications

References 25 publications

The Process of Acclimation to Chronic Hypoxia Leads to Submandibular Gland and Periodontal Alterations: An Insight on the Role of Inflammatory Mediators

The Process of Acclimation to Chronic Hypoxia Leads to Submandibular Gland and Periodontal Alterations: An Insight on the Role of Inflammatory Mediators

Porphyromonas gingivalis bypasses epithelial barrier and modulates fibroblastic inflammatory response in an in vitro 3D spheroid model

Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications

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Deleterious effect of chronic continuous hypoxia on oral health

Cited by 10 publications

References 25 publications

The Process of Acclimation to Chronic Hypoxia Leads to Submandibular Gland and Periodontal Alterations: An Insight on the Role of Inflammatory Mediators

The Process of Acclimation to Chronic Hypoxia Leads to Submandibular Gland and Periodontal Alterations: An Insight on the Role of Inflammatory Mediators

Porphyromonas gingivalis bypasses epithelial barrier and modulates fibroblastic inflammatory response in an in vitro 3D spheroid model

Electrospun Composite Bead-on-String Nanofibers Containing CaO2 Nanoparticles and MnO2 Nanosheets as Oxygen-Release Systems for Biomedical Applications

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Product

Resources

About

Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications