Delayed Ripening Tomato Plants Expressing the Enzyme 1-Aminocyclopropane-1-carboxylic Acid Deaminase. 1. Molecular Characterization, Enzyme Expression, and Fruit Ripening Traits

Reed, Andrew J.; Magin, Kimberly M.; Anderson, Jerry S.; Austin, Glenn D.; Rangwala, Tasneem; Linde, D. C.; Love, John N.; Rogers, Stephen L.; Fuchs, Roy L.

doi:10.1021/jf00055a036

Cited by 31 publications

(29 citation statements)

References 22 publications

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“…The (MT× ACD ) F 1 generation was PCR screened for the presence of both genes using the following sets of primers: ACD forward, 5ʹ-CGATACGCTGGTTTCCATC-3ʹ, and reverse, 5ʹ-CGTCCTCTTCCGTAATCTCG-3ʹ; and Mi-1.2 forward, 5ʹ-CTAGAAAGTCTGTTTGTGTCTAACAAAGG-3ʹ, and reverse, 5ʹ-CTAAGAGGAATCTCATCACAGG-3ʹ. Similar to Mi-1.2 , the ACD transgene behaves as a single dominant gene inherited in a Mendelian fashion when transgenic tomato plants are crossed with other tomato lines ( Reed et al , 1995 ); therefore, heterozygous F 1 plants were used for subsequent assays.…”

Section: Methodsmentioning

confidence: 99%

The ethylene response factor Pti5 contributes to potato aphid resistance in tomato independent of ethylene signalling

Avila

Goggin

2014

Journal of Experimental Botany

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Section: Methodsmentioning

confidence: 99%

The ethylene response factor Pti5 contributes to potato aphid resistance in tomato independent of ethylene signalling

Avila

Goggin

2014

Journal of Experimental Botany

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“…Furthermore, when Reed et al (1995) transformed tomato plants with a binary vector, in which the T-DNA was flanked only by a RB, a transformant was obtained in which the complete vector and two additional tandem, incomplete copies of the studied gene on the T-DNA were integrated at a single locus. Denis et al (1995) studied 14 Brassica napus plants transformed with a binary vector with a T-DNA flanked by a RB and a LB in normal positions.…”

Section: Integration Of More Than One Unit Of Binary Vector Dnamentioning

confidence: 99%

Fluorescence in situ hybridization on extended DNA fibres as a tool to analyse complex T‐DNA loci in potato

et al. 1998

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SummaryIn this study the T-DNA composition of four antisense potato transformants showing complete or very strong inhibition of granule-bound starch synthase (GBSS) activity was analysed in detail. By Southern blot hybridizations, it was determined that all four transformants contained T-DNAs on multiple linkage groups and that most linkage groups contained multiple T-DNA copies, often in combination with non-T-DNA vector sequences. Subsequently, fluorescence in situ hybridization was performed on extended DNA fibres ('fibre-FISH') of three progeny plants each containing a single linkage group with a complex T-DNA organization. By using two differently labelled probes, one consisting of T-DNA sequences and the other of vector DNA sequences, it was possible to visualize the composition of complex loci. DNA sequences of 5-6 kb were well distinguishable. With this technique it is possible to determine T-DNA copy number, and arrangement of T-DNA and vector DNA sequences in a locus, more accurately than by Southern blot analysis alone. Therefore, fibre-FISH is a valuable supplementary tool to study T-DNA integrations in detail.

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“…In addition, many transgenic plants expressing bacterial acdS genes have pinpointed the beneficial roles of AcdS on plant growth and environmental adaptation (reviewed by Gontia-Mishra et al, 2014). For example, tomato plants expressing acdS genes from Pseudomonas chlororaphis 6G5 exhibit reduced sensitivity to ethylene compared with wild-type plants (Klee et al, 1991;Reed et al, 1995). Recently, the acdS gene from Pseudomonas was shown to confer increased salt tolerance in transgenic canola (Sergeeva et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Rapid degradation ofPseudomonas fluorescens1-aminocyclopropane-1-carboxylic acid deaminase proteins expressed in transgenicArabidopsis

Kim

Park

Chae

et al. 2014

FEMS Microbiol Lett

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1-Aminocyclopropane-1-carboxylate (ACC) deaminase is commonly produced by plant growth-promoting rhizobacteria (PGPR) and has been suggested to facilitate the growth and stress tolerance of hosts via a reduction in levels of ethylene. However, the regulatory mechanism of ACC deaminase (AcdS) protein within host plant cells is largely unknown. Here, we demonstrated beneficial effects and post-translational modification of PGPR-originated AcdS proteins in plants. Compared with the wild-type, transgenic Arabidopsis expressing the Pseudomonas fluorescens acdS (PfacdS) gene displayed increased root elongation and reduced sensitivity to 10 μM exogenous ACC, an ethylene precursor. Arabidopsis expressing PfacdS also showed increased tolerance to high salinity (150 mM NaCl). PfAcdS proteins accumulated in transgenic Arabidopsis were rapidly degraded, which was potentially mediated by the 26S proteasome pathway. The degradation of PfAcdS was alleviated in the presence of exogenous ACC. In conclusion, our data suggest that the plant growth-promoting effects of bacterial AcdS proteins are potentially modulated via protein turnover inside the host plant cells. Such post-translational modification plays a physiological role in the mutualistic interactions between microorganisms and plants in the rhizospheric and/or endospheric niche.

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Delayed Ripening Tomato Plants Expressing the Enzyme 1-Aminocyclopropane-1-carboxylic Acid Deaminase. 1. Molecular Characterization, Enzyme Expression, and Fruit Ripening Traits

Cited by 31 publications

References 22 publications

The ethylene response factor Pti5 contributes to potato aphid resistance in tomato independent of ethylene signalling

The ethylene response factor Pti5 contributes to potato aphid resistance in tomato independent of ethylene signalling

Fluorescence in situ hybridization on extended DNA fibres as a tool to analyse complex T‐DNA loci in potato

Rapid degradation ofPseudomonas fluorescens1-aminocyclopropane-1-carboxylic acid deaminase proteins expressed in transgenicArabidopsis

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