Rapid degradation of<i>Pseudomonas fluorescens</i>1-aminocyclopropane-1-carboxylic acid deaminase proteins expressed in transgenic<i>Arabidopsis</i>

Kim, Kang-Min; Park, Sung-Hee; Chae, Jong‐Chan; Soh, Byoung Yul; Lee, Kui-Jae

doi:10.1111/1574-6968.12456

Cited by 10 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). These data are in accordance with the previous report that compared to non-transgenic plants, transgenic Arabidopsis expressing the P. fluorescens ACCD gene, displayed increased tolerance against high salinity at 150 mM NaCl (Kim et al 2014).…”

Section: Discussionsupporting

confidence: 93%

Functional analysis of the 1-aminocyclopropane-1-carboxylate deaminase gene of the biocontrol fungus Trichoderma asperellum ACCC30536

et al. 2016

View full text Add to dashboard Cite

1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD) cleaves ACC, the immediate precursor of the ethylene, decreasing the level of ethylene and inhibition of plant growth resulted by environmental stresses. Here, TaACCD was cloned from the biocontrol agent Trichoderma asperellum ACCC30536. Its open reading frame was 1047 bp long encoding a 37 kD protein of 348 aa, and a pI of 5.77. Phylogenetic analysis demonstrated this protein to be closely related to ACCD from T. asperellum T203 (ACX94231). Transformation of Populus davidiana × P. bolleana with TaACCD, increased salinity tolerance of transgenic plants Pdb-ACCD3 and Pdb-ACCD5. Transgenic plants could survive at salinity of 200 mM NaCl, whereas untransformed control poplar Pdb-NT could withstand salinity to 150 mM NaCl. Transformed plants accumulated higher amounts of chlorophyll compared to Pdb-NT plants. Accumulation of reactive oxygen species (ROS) was regulated by TaACCD under salt stress, as shown from higher superoxide dismutase (SOD) and peroxidase (POD) activities, as well as NBT and DAB staining. Evans blue staining showed that TaACCD maintained membrane integrity in Populus under salt stress conditions. Additionally, TaACCD expression decreased ethylene content of transgenic plants compared to nontransgenic plants, but salt content in plant leaves didnt show obvious difference under same salt concentration. To the best of our knowledge, the current study is the first demonstration that the TaACCD gene from T. asperellum ACCC30536 can enhance tolerance of Populus to salt stress.Key words: Trichoderma asperellum, 1-aminocyclopropane-1-carboxylate deaminase, transgenic poplar, salinity tolerance.Résumé : La 1-aminocyclopropane-1-carboxylate (ACC) désaminase (ACCD) scinde l'ACC, précurseur immédiat de l'éthylène, ce qui réduit la concentration de ce gaz et l'effet inhibiteur qu'il a sur la croissance des plantes soumises à un stress environnemental. Dans le cadre de cette étude, les auteurs ont cloné le gène TaACCD de l'agent de lutte biologique Trichoderma asperellum ACCC30536. Son cadre de lecture ouvert mesure 1 047 pb de longueur et code une protéine de 37 kD composée de 348 acides aminés et d'un pI de 5,77. L'analyse phylogénétique révèle que la protéine est étroitement apparentée à l'ACCD de T. asperellum T203 (ACX94231). La modification de Populus davidiana × P. bolleana avec le gène TaACCD augmente la tolérance des plants transgéniques Pdb-ACCD3 et Pdb-ACCD5 à la salinité. Ces plants survivent à une salinité de 200 mM de NaCl, contre 150 mM de NaCl pour le peuplier témoin non modifié Pdb-NT. Les plants modifiés accumulent plus de chlorophylle que les plants de Pdb-NT. Le gène TaACCD régule l'accumulation des espèces réactives d'oxygène résultant du stress dû au sel, comme l'indique la plus grande activité de la superoxyde dismutase (SOD) et de la peroxydase (POD), de même que la coloration au NBT et au DAB. La coloration au bleu d'Evans révèle que le gène TaACCD aide la membrane cellulaire à garder son intégrité chez Populus, dans de...

show abstract

Section: Discussionsupporting

confidence: 93%

Functional analysis of the 1-aminocyclopropane-1-carboxylate deaminase gene of the biocontrol fungus Trichoderma asperellum ACCC30536

et al. 2016

View full text Add to dashboard Cite

show abstract

“…ET functions in the regulation of various physiological processes such as seed germination and leaf senescence as well as abiotic and biotic stress responses [ 39 , 40 , 41 , 42 ]. During abiotic stress, ET signaling is required for plant adaption to salt and drought stress [ 43 , 44 ], nevertheless overproduction of cellular ET reduces the tolerance of plants to the stress [ 45 , 46 , 47 ]. A generalized role of ET has not been established during biotic stress.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Transcription Factor MYB102 Increases Plant Susceptibility to Aphids by Substantial Activation of Ethylene Biosynthesis

Zhu

Guo

et al. 2018

Biomolecules

View full text Add to dashboard Cite

Induction of ethylene biosynthesis by aphids increases the susceptibility of several plant species to aphids. Recent studies have indicated that some MYB transcription factors regulate the phloem-based defense against aphid infestation by modulating ethylene (ET) signaling. Arabidopsis MYB102 has previously been shown to be induced by wound signaling and regulate defense response against chewing insects. However, it remains unclear whether Arabidopsis MYB102 takes part in the defense response of plants to aphids. Here, we investigated the function of MYB102 in the response of Arabidopsis to aphid infestation. Arabidopsis MYB102 was primarily expressed in vascular tissues, and its transcription was remarkably induced by green peach aphids (GPA; Myzus persicae). The results of RNA-Sequencing revealed that overexpression of MYB102 in Arabidopsis promoted ET biosynthesis by upregulation of some 1-aminocyclopropane-1-carboxylate synthase (ACS) genes, which are rate-limiting enzymes of the ET-synthetic pathway. Enhanced ET levels led to reduced Arabidopsis resistance to GPA. Furthermore, dominant suppression of MYB102 inhibited aphid-induced increase of ET levels in Arabidopsis. In agreement with a negative regulatory role for ET in aphid defense responses, the MYB102-overexpressing lines were more susceptible to GPA than wild-type (WT) plants. Overexpression of MYB102 in Arabidopsis obviously repressed aphid-induced callose deposition. Conversely, overexpression of MYB102 failed to increase aphid susceptibility in both the ET-insensitive mutants and plants treated with inhibitors of ET signaling pathways, demonstrating that the ET was critical for promoting aphid performance conferred by overexpression of MYB102. Collectively, our findings indicate that the Arabidopsis MYB102 increases host susceptibility to GPA through the ET-dependent signaling pathways.

show abstract

“…All indicate a SIT1-MPK3/6 cascade mediates salt sensitivity by regulating ethylene homeostasis. In addition, plant growth-promoting rhizobacteria (PGPR) produced AcdS (ACC deaminase), which facilitated the growth and stress tolerance of hosts via a reduction in levels of ethylene ( Ali et al, 2014 ; Barnawal et al, 2014 ; Kim et al, 2014 ). Transgenic Arabidopsis expressing AcdS showed reduced sensitivity to exogenous ACC but increased tolerance to high salinity.…”

Section: Ethylene Biosynthesis and Salinity Stressmentioning

confidence: 99%

The Role of Ethylene in Plants Under Salinity Stress

et al. 2015

View full text Add to dashboard Cite

Although the roles of ethylene in plant response to salinity and other stresses have been extensively studied, there are still some obscure points left to be clarified. Generally, in Arabidopsis and many other terrestrial plants, ethylene signaling is indispensable for plant rapid response and tolerance to salinity stress. However, a few studies showed that functional knock-out of some ACSs increased plant salinity-tolerance, while overexpression of them caused more sensitivity. This seems to be contradictory to the known opinion that ethylene plays positive roles in salinity response. Differently, ethylene in rice may play negative roles in regulating seedling tolerance to salinity. The main positive ethylene signaling components MHZ7/OsEIN2, MHZ6/OsEIL1, and OsEIL2 all negatively regulate the salinity-tolerance of rice seedlings. Recently, several different research groups all proposed a negative feedback mechanism of coordinating plant growth and ethylene response, in which several ethylene-inducible proteins (including NtTCTP, NEIP2 in tobacco, AtSAUR76/77/78, and AtARGOS) act as inhibitors of ethylene response but activators of plant growth. Therefore, in addition to a summary of the general roles of ethylene biosynthesis and signaling in salinity response, this review mainly focused on discussing (i) the discrepancies between ethylene biosynthesis and signaling in salinity response, (ii) the divergence between rice and Arabidopsis in regulation of salinity response by ethylene, and (iii) the possible negative feedback mechanism of coordinating plant growth and salinity response by ethylene.

show abstract

Rapid degradation ofPseudomonas fluorescens1-aminocyclopropane-1-carboxylic acid deaminase proteins expressed in transgenicArabidopsis

Cited by 10 publications

References 52 publications

Functional analysis of the 1-aminocyclopropane-1-carboxylate deaminase gene of the biocontrol fungus Trichoderma asperellum ACCC30536

Functional analysis of the 1-aminocyclopropane-1-carboxylate deaminase gene of the biocontrol fungus Trichoderma asperellum ACCC30536

Arabidopsis Transcription Factor MYB102 Increases Plant Susceptibility to Aphids by Substantial Activation of Ethylene Biosynthesis

The Role of Ethylene in Plants Under Salinity Stress

Contact Info

Product

Resources

About