Delayed Re-Epithelialization in Periostin-Deficient Mice during Cutaneous Wound Healing

Abstract

BackgroundMatricellular proteins, including periostin, are important for tissue regeneration.Methods and FindingsPresently we investigated the function of periostin in cutaneous wound healing by using periostin-deficient (−/−) mice. Periostin mRNA was expressed in both the epidermis and hair follicles, and periostin protein was located at the basement membrane in the hair follicles together with fibronectin and laminin γ2. Periostin was associated with laminin γ2, and this association enhanced the proteolytic cleavage of the laminin γ2 long form to produce its short form. To address the role of periostin in wound healing, we employed a wound healing model using WT and periostin−/− mice and the scratch wound assay in vitro. We found that the wound closure was delayed in the periostin−/− mice coupled with a delay in re-epithelialization and with reduced proliferation of keratinocytes. Furthermore, keratinocyte proliferation was enhanced in periostin-overexpressing HaCaT cells along with up-regulation of phosphorylated NF-κB.ConclusionThese results indicate that periostin was essential for keratinocyte proliferation for re-epithelialization during cutaneous wound healing.

Influence of Periostin on Synoviocytes in Knee Osteoarthritis

“…This protein has known functions in osteology, tissue repair, oncology, cardiovascular and respiratory systems and various inflammatory settings. According to recent reports, periostin deposition promotes chronic allergic inflammation by activating nuclear factor kappa B (NF-ĸB) signaling (9)(10)(11). Periostin is considered to be an important structural mediator by balancing appropriate versus inappropriate tissue adaptation in response to insult/injury.…”

Functional significance of periostin in excisional skin repair

“…Beginning with Nishiyama and colleagues, 12 followed by our report 13 and finally Ontsuka et al, 14 each report shows a significant delay in the closure of excisional wounds in the absence of periostin. From these publications, it is clear that the wound closure results are reproducible even in different derivations of the knockout mouse.…”

“…Nishiyama and colleagues were the first to report a delay in dermal wound repair due to the absence of periostin. 12 This delay was observed at days 3, 5 and 8 following 3 mm full-thickness excisional wounding. The authors attributed this delay in wound closure to a defect in reepithelialization, brought on by reduced keratinocyte proliferation in the hair follicles ( Table 1 and Fig.…”

Avaliação da cicatrização cutânea: fluorescência e estereologia

“…A derme e o tecido tegumentar mostraram, finalmente, depósito abundante de colágeno, com fibras mais espessas e compactas, com poucas células produtoras de tecido conjuntivo, os fibrócitos (Junqueira e Carneiro, 1985). (Nishiyama et al, 2011).…”

“…Isto pode ser explicado pela presença de queratinócitos indiferenciados nos anexos epidérmicos. Esses queratinócitos são ativados por sinalizadores de replicação, sendo que, após esse comando, as camadas epidérmicas e os anexos cutâneos sofrem, simultaneamente, proliferação celular (Nishiyama et al, 2011). Tais dados foram confirmados com a marcação imunoistoquímica com PCNA, onde células presentes da epiderme, especialmente na camada basal, e na parede dos anexos apresentavam marcação mais intensa.…”