Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate

Song, Hyun-Ouk; Lim, Young-Su; Moon, Sun-Joo; Ahn, Myoung-Hee; Ryu, Jae‐Sook

doi:10.3347/kjp.2010.48.1.1

Cited by 18 publications

(20 citation statements)

References 28 publications

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“…Controversial studies have been published concerning cytokines profile that may be induced in response to T. vaginalis infection. Some previous studies in vitro revealed that exposure to T. vaginalis stimulated IL-8 secretion in human monocytes, [21] neutrophils, [22] and vaginal epithelial cells. [23] It was further demonstrated that the cytokines IL-2 and IFN-γ were secreted by murine lymphocytes infected with T. vaginalis.…”

Section: Discussionmentioning

confidence: 99%

Immunological Aspects of Trichomonas vaginalis Infection in Women Attending Maternity Teaching Hospital and Some Public Health Centers in Erbil Governorate, Northern Iraq

Nouraddin¹,

Alsakee

2019

Cihan U Erbil SCI J

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Trichomonas vaginalis infection is one of the sexually transmitted diseases. It is a health problem all over the world, including Iraq. It has also been associated with adverse outcomes of pregnancy and increased risk of HIV. Trichomoniasis typically elicits aggressive local cellular immune responses with inflammation of the vaginal epithelium and exocervix in women and urethra in men. The present study was aimed to assess serum level of interferon gamma (IFN-γ), Interleukin 10 (IL-10), C-reactive protein, antiphospholipid, anticardiolipin antibodies with eosinophil count as well. Four hundred and forty women with ages ranging between 16 and 60 years old (average of 34.2 years) who attended Maternity Teaching Hospital and a number of public health centers in Erbil Governorate were screened for trichomoniasis by direct wet mount preparation and culture technique. Serum IFN-γ, IL-10, antiphospholipid, and anticardiolipin were assessed by enzyme-linked immunosorbent assay. C-reactive protein (CRP) and eosinophil count were assessed by I CROMA and Coulter, respectively. The results revealed that IL-10 level (96.46 ± 1.97 pg/ml vs. 91.86 ± 1.48 pg/ml), eosinophil count (0.1 ± 0.07 × 10³/μL vs. 0.1 ± 0.01 × 10³/μL), and CRP concentration (2.55 ± 0.74 mg/l vs. 2.27 ± 0.37 mg/l) were non-significantly (P > 0.05) changed in infected women in comparison with negative control group. However, serum IFN-γ level (484.83 ± 38.35 pg/ml vs. 372.15 ± 9.49 pg/ml) was significantly (P < 0.05) elevated in infected women in comparison to the control group. The results also revealed significant increase (P < 0.05) of antiphospholipid immunoglobulin G (IgG) antibodies (4.75 ± 0.3 U/ml vs. 3.79 ± 0.11 U/ml) in the sera of infected women, but antiphospholipid immunoglobulin M (IgM), anticardiolipin IgG, and anticardiolipin IgM antibodies were non-significantly altered in response to T. vaginalis infection.

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Section: Discussionmentioning

confidence: 99%

Immunological Aspects of Trichomonas vaginalis Infection in Women Attending Maternity Teaching Hospital and Some Public Health Centers in Erbil Governorate, Northern Iraq

Nouraddin¹,

Alsakee

2019

Cihan U Erbil SCI J

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“…T. vaginalis lysate and ESP were prepared as described previously [17] . To prepare the lysate, T. vaginalis trophozoites were harvested in logarithmic growth phase and washed three times in PBS (pH 6.2).…”

Section: Methodsmentioning

confidence: 99%

Trichonomas vaginalis Metalloproteinase Induces Apoptosis of SiHa Cells through Disrupting the Mcl-1/Bim and Bcl-xL/Bim Complexes

et al. 2014

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To elucidate the roles of metalloproteinases and the Bcl-2 family of proteins in Trichovaginalis. vaginalis-induced apoptosis in human cervical cancer cells (SiHa cells) and vaginal epithelial cells (MS74 cells), SiHa cells and MS74 cells were incubated with live T. vaginalis, T. vaginalis excretory and secretory products (ESP), and T. vaginalis lysates, either with or without the specific metalloproteinase inhibitor 1,10-phenanthroline (1,10-PT), and examined apoptotic events and Bcl-2 signaling. The live T. vaginalis and the T. vaginalis ESP induced the release of cytochrome c into the cytosol, the activation of caspase-3 and caspase-9, and the cleavage of PARP. Additionally, the live T. vaginalis, but not the T. vaginalis lysate, induced the cleavage of the proapoptotic Bim protein. The live T. vaginalis and the T. vaginalis ESP, but not the T. vaginalis lysate, induced the dose-dependent cleavage of the antiapoptotic Bcl-xL and Mcl-1 proteins and decreased the association levels of Bcl-xL/Bim and Mcl-1/Bim complexes. We performed gelatin zymography and casein-hydrolysis assays on the live T. vaginalis and the T. vaginalis ESP to identify the apoptosis-inducing factor. Both the live T. vaginalis and the ESP contained high levels of metalloproteinases, of which activities were significantly inhibited by 1,10-PT treatment. Furthermore, the 1,10-PT blocked the cleavage of Bcl-xL, Mcl-1, PARP, caspase-3, and caspase-9, as well as the release of cytochrome c into the cytosol, and it significantly increased the association levels of the Bcl-xL/Bim and Mcl-1/Bim protein complexes, returning them to normal levels. Our results demonstrate that T. vaginalis induces mitochondria-dependent apoptosis in SiHa cells through the dissociation of Bcl-xL/Bim and Mcl-1/Bim complexes and that the apoptosis is blocked by the metalloproteinase inhibitor 1,10-PT. These results expand our understanding of the role of metalloproteinases in T. vaginalis-induced apoptosis and the signaling pathway in trichomoniasis of the cervicovaginal epithelial cells.

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“…Stimulation with TvSP for IL-8 production in neutrophils Neutrophils (1 9 10 5 ) were seeded in 96-well tissue culture plates and then directly incubated for the times indicated with or without TvSP [6], pretreated TvSP or modified TvSP at 37°C in a 5% CO 2 incubator. No cytotoxicity of lipase or proteinase K alone was observed.…”

Section: Isolation Of Human Neutrophilsmentioning

confidence: 99%

“…In addition, in-vitro experiments indicate that actively rotating T. vaginalis harbors and secretes various immunological stimuli, which might lead to vaginal mucosal inflammation. One study showed that the longevity of human neutrophils is prolonged in response to T. vaginalis lysate [6]. Another study showed that human neutrophils or macrophages stimulated with live T. vaginalis produce the chemokine interleukin (IL)-8 and the proinflammatory cytokines tumor necrosis factor-a, IL-6, and IL-1b [7,8].…”

Section: Introductionmentioning

confidence: 99%

Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products

et al. 2012

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Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate

Cited by 18 publications

References 28 publications

Immunological Aspects of Trichomonas vaginalis Infection in Women Attending Maternity Teaching Hospital and Some Public Health Centers in Erbil Governorate, Northern Iraq

Immunological Aspects of Trichomonas vaginalis Infection in Women Attending Maternity Teaching Hospital and Some Public Health Centers in Erbil Governorate, Northern Iraq

Trichonomas vaginalis Metalloproteinase Induces Apoptosis of SiHa Cells through Disrupting the Mcl-1/Bim and Bcl-xL/Bim Complexes

Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products

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