Delayed fracture healing in tetranectin-deficient mice

Abstract

Tetranectin is a plasminogen-binding protein that enhances plasminogen activation, which has been suggested to play a role in tissue remodeling. Recently, we showed that tetranectin has a role in the wound-healing process. In this study, we investigated whether tetranectin plays a role in fracture healing. The fracture-healing process was studied using a femoral osteotomy model in tetranectin-null mice, previously generated by the authors. Radiographic imaging, micro-computed tomography (μCT), and histological analysis were used to evaluate osteotomy healing. In wild-type mice, a callus was apparent from 7 days, and most samples showed marked callus formation and rebridging of the cortices at the osteotomy site at 21 days. In contrast, in the tetranectin-null mice there was no callus formation at 7 days and much less callus formation and no bridging of cortices were observed at 21 days. At 35 days, all osteotomy sites showed clear rebridging, and secondary bone formation was achieved in wild-type mice by 42 days. In contrast, no clear rebridging or secondary bone formation was observed at 42 days in the tetranectin-null mice. Analysis using μCT at 21 days after osteotomy revealed that the callus area in tetranectin-null mice was smaller than that in wild-type mice. Histological analysis also showed that soft tissue and callus formation were smaller in the tetranectin-null mice at the early stage of the healing process after drill-hole injury. These results suggested that tetranectin could have a role in the positive regulation at the early stage of the fracture-healing process, which was reflected in the delayed fracture healing in tetranectin-deficient mice.

Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold

“…3A, B and Supplemental Table 2). SPP1 and CLEC3B are genes that are involved in osteogenic processes (25)(26)(27)(28). The ability to produce proteins that are specific for bone formation was demonstrated by immunocytochemical analysis that revealed, in particular, an expression of osteopontin in cells that were seeded on biomaterial at d 9.…”

Calmodulin Regulated Spectrin Associated Protein 1 mRNA is Directly Regulated by miR‐126 in Primary Human Osteoblasts

“…Nonetheless, the 19 genes that complied with the criteria were interesting, since they are either directly involved in osteoblast biology or in osteoblast‐relevant processes such as ion channels/vesicle trafficking or extracellular matrix biology. Genes involved in bone metabolism included neurofibrin 1 [El Khassawna et al, ], tetranectin [Wewer et al, ; Iba et al, ,, ], and tumor necrosis receptor superfamily member TNFRSF11B [Osta et al, ] and TNF‐α induced protein TNFAIP6 [Inoue et al, ; Tsukahara et al, ]. Genes involved in extracellular matrix formation and turnover were the above‐mentioned TNFAIP6 and tetranectin, as well as PLAUR [Furlan et al, ].…”

Tetranectin as a Potential Biomarker for Stable Coronary Artery Disease

“…TN has received considerable attention because of its function in plasminogen activation 18 19 . Atherosclerosis is a chronic inflammatory-fibroproliferative arterial disease.…”