Delayed ERK activation by ceramide reduces melanin synthesis in human melanocytes

Kim, Dong-Seok; Kim, Sook-Young; Chung, Jin‐Ho; Kim, Kyu-Han; Eun, Hee-Chul; Park, Kyoung-Chan

doi:10.1016/s0898-6568(02)00024-4

Cited by 150 publications

(106 citation statements)

References 38 publications

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“…The phosphorylated CREB then binds to the CRE site on the MITF promoter, interacts with CREB binding protein (CBP) to increase the expression of MITF, and eventually causes melanogenesis (10,11). However, the ERK and Akt signaling pathways have been shown to negatively regulate melanogenesis in melanocytes (12,13). Inhibition of ERK and PI3K/Akt can cause the stimulation of melanogenesis (14,15).…”

Section: Introductionmentioning

confidence: 99%

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

Cheng

Jin

et al. 2015

International Journal of Molecular Medicine

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Abstract. Melia azedarach (MA) has been used in folk medicine in Asia for the treatment of several diseases. Several constituents from MA possess anti-herpetic, anti-angiogenic and anticancer properties. The aim of the present study was to investigate the effect of a 70% ethanol extract of MA on melanogenesis and the underlying mechanisms involved. A B16F10 mouse melanoma cell line was used in our experiments. Treatment of B16F10 cells with the MA extract (10, 20 and 40 µg/ml) increased melanin content in a concentration-dependent manner without cytotoxicity at 24 h. Further experiments indicated that the MA extract (20 µg/ml) increased melanin content as early as at 4 h after treatment. Additionally, although the MA extract did not affect intracellular tyrosinase activity and the protein levels of tyrosinase and tyrosinase-related protein-2 (TRP-2) at 2 and 4 h after treatment, the MA extract increased TRP-1 protein expression at both time points. However, no significant effect of the MA extract treatment on TRP-1 mRNA level at the time points measured was observed. In conclusion, the results from the present study demonstrate that the MA extract increases melanogenesis through the upregulation of TRP-1 protein expression by post-transcriptional control in B16F10 cells and suggest that the MA extract can be viewed as a rapid inducer of melanogenesis, thus rendering it a potential treatment for hypopigmentation diseases including vitiligo.

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Section: Introductionmentioning

confidence: 99%

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

Cheng

Jin

et al. 2015

International Journal of Molecular Medicine

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“…In particular, the MAPK kinase (MEK)/extracellular signal-regulated kinase (ERK) and the PI3K/Akt pathways have been shown to negatively regulate melanin synthesis in melanocytes and melanoma cells [14][15][16][17][18] . Currently, cosmetic products possessing whitening effects occupy a large proportion of the cosmetic market and have become more popular than ever in Asia.…”

Section: Introductionmentioning

confidence: 99%

[6]-Shogaol inhibits melanogenesis in B16 mouse melanoma cells through activation of the ERK pathway

Cheng

et al. 2012

Acta Pharmacol Sin

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Aim:To investigate the effect of [6]-shogaol, an active ingredient in ginger, on melanogenesis and the underlying mechanisms. Methods: B16F10 mouse melanoma cells were tested. Cell viability was determined with the MTT assay. Melanin content and tyrosinase activity were analyzed with a spectrophotometer. The protein expression of tyrosinase and microphthalmia associated transcription factor (MITF), as well as phosphorylated or total ERK1/2 and Akt were measured using Western blot.

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“…[13][14][15] Several studies have found that ERK activation induces the phosphorylation of MITF at serine 73, which leads to ubiquitination of it followed by proteasome-mediated degradation. [14][15][16] It has reported that sustained activation of ERK by sphingosine-1-phosphate (S1P) 35) and C 2 -ceramide 40) induced MITF A, After serum starvation for 16 h, cells were pretreated with CEFV (100 mg/mL) for the times indicated and stimulated with -MSH for 30 min. Whole-cell lysates were then subjected to Western blot analysis using an antibody against phospho-specific ERK1/2 (p-ERK1/2).…”

Section: Discussionmentioning

confidence: 99%

FermentedViola mandshuricaInhibits Melanogenesis in B16 Melanoma Cells

Kwak

Kim

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Delayed ERK activation by ceramide reduces melanin synthesis in human melanocytes

Cited by 150 publications

References 38 publications

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

[6]-Shogaol inhibits melanogenesis in B16 mouse melanoma cells through activation of the ERK pathway

FermentedViola mandshuricaInhibits Melanogenesis in B16 Melanoma Cells

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