[6]-Shogaol inhibits melanogenesis in B16 mouse melanoma cells through activation of the ERK pathway

Abstract

Aim:To investigate the effect of [6]-shogaol, an active ingredient in ginger, on melanogenesis and the underlying mechanisms. Methods: B16F10 mouse melanoma cells were tested. Cell viability was determined with the MTT assay. Melanin content and tyrosinase activity were analyzed with a spectrophotometer. The protein expression of tyrosinase and microphthalmia associated transcription factor (MITF), as well as phosphorylated or total ERK1/2 and Akt were measured using Western blot.

Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells

“…The past two decades have brought an enormous increase in our understanding of melanogenic enzymes, of melanosomes, and of the intracellular and intercellular signaling pathways that regulate pigmentation (Park, Kosmadaki, Yaar, & Gilchrest, 2009). The B16 mouse melanoma cell line is used extensively for melanogenic studies and is considered a stable cell line for studying melanin production (Kim et al, 2014;Sato, Tsukimoto, Shimura, Awaya, & Kojima, 2011;Yao, Oh, Oh, Park, & Chung, 2013).…”

Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation

“…Stimulation of c-kit signaling has also been reported to induce MITF protein degradation, which is prevented by the MAPK/ERK signaling pathway inhibitor, PD98059 (30). Moreover, inhibition of the ERK signaling pathway by PD98059 has been found to induce melanogenesis in B16F10 cells (25). It is well established that the PI3K/Akt signaling pathway is important in the regulation of various cellular processes, including cell growth and apoptosis (31)(32).…”

“…Analysis of melanin content. Melanin content was measured as described previously, with slight modifications (25). B16F10 cells were incubated overnight with DMEM (phenol red-free) containing 10% FBS.…”

UV irradiation-induced production of monoglycosylated biglycan through downregulation of xylosyltransferase 1 in cultured human dermal fibroblasts

“…Cells were lysed with lysis buffer (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM ethylenediaminetetraacetic acid, 1 mM ethyleneglycoltetraacetic acid, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM Na 3 VO 4 , 1 mM dithiothreitol, 10 mg/ml leupeptin, 10 mg/ml aprotinin, and 1 mM phenylmethanesulfonyl fluoride) [28]. Insoluble debris was removed by centrifugation at 12,000 rpm for 10 min, and the total protein samples were fractionated and transferred as described above, and analyzed by Western blotting using anti-BGN antibodies.…”