Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18

FangYan, Jiang; Wang, Jinmei; Kaleem, Imdad; Dai, Dongfang; Zhou, Xiaohong; Li, Chun

doi:10.1016/j.biortech.2011.05.050

Cited by 41 publications

(19 citation statements)

References 27 publications

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“…Traditional chemical degumming processes have the disadvantages of high energy requirement, need of large amounts of water and chemicals, loss of nutrients, and disposal of highly polluted effluent [13]. Enzymatic degumming was first introduced by the German Lurgi Company as BEnzyMax process^in which a phospholipase was used to convert nonhydratable phospholipids into their hydratable forms, which were then removed by centrifugation [14,15]. Enzymatic degumming overcomes the drawbacks in traditional degumming processes, offering advantages of reduction in the amounts of acid and base used and wastewater generated during the refining process, and an enhancement in product yields [16].…”

Section: Introductionmentioning

confidence: 99%

“…So far, several kinds of phospholipases have been commercialized for oil degumming, for example, Lecitase® 10 L (PLA 2 ), Lecitase® Novo (PLA 1 ), Lecitase® Ultra (PLA 1 ), Purifine® (PLC), and LysoMax® (lipid acyl transferase with PLA 2 activity) [17,18]. Although the phosphorus content in oil can be reduced remarkably after treatment by Lecitase® Ultra and Lecitase® Novo, these two PLA 1 s exhibit both phospholipase and lipase activity, which causes a decrease in oil yield by hydrolysis of triglycerides [7,8,14]. Thus, PLA 2 s without lipase activity are more promising for oil degumming in considering oil yield [19].…”

Section: Introductionmentioning

confidence: 99%

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Streptomyces violaceoruber Phospholipase A2: Expression in Pichia pastoris, Properties, and Application in Oil Degumming

Liu

Xiang

Chen

et al. 2015

Appl Biochem Biotechnol

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The phospholipase A2 (PLA2) from Streptomyces violaceoruber was successfully expressed in the methylotrophic yeast Pichia pastoris GS115 under the control of AOX1 promoter for the first time. The maximum activity of the recombinant PLA2 (rPLA2) reached 34.7 ± 0.2 U/mL, and specific activity was 170 ± 4 U/mg after purification. On the sodium dodecyl sulfate polyacrylamide gel electrophoresis of the culture supernatants, three bands of 21, 18, and 14.3 kDa were observed. The peptide mass fingerprinting analysis showed that all of these three bands were rPLA2 from S. violaceoruber. By the treatment with Endo H and PNGase F, it indicated that the rPLA2 occurred N-glycosylation. The enzymatic properties of this enzyme were determined. The rPLA2 exhibited a lower optimum pH (pH = 6.0) compared to the wild-type enzyme, which was a desirable property in the application of oil degumming. In the enzymatic degumming process, the phosphorus content decreased from 261.77 ± 3.51 mg/kg to 20.74 ± 0.23 mg/kg, which is very promising for the industrial application.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Streptomyces violaceoruber Phospholipase A2: Expression in Pichia pastoris, Properties, and Application in Oil Degumming

Liu

Xiang

Chen

et al. 2015

Appl Biochem Biotechnol

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“…Em comparação com os processos tradicionais, a degomagem enzimática tem muitas vantagens (JIANG et al, 2011). Além da redução nas quantidades de ácido e base utilizados, há uma redução em efluentes gerados durante o processo de refino.…”

Section: O Desenvolvimento Regional Das Pequenas E Médias Propriedadeunclassified

“…As fosfolipases (A1, A2, C e D) são um grupo importante de enzimas que hidrolisam fosfolipídios, causando a liberação de uma variedade de produtos, como, por exemplo, os liso-fosfolipídios, os ácidos graxos livres, os di-acilgliceróis, colina fosfato (DE MARIA et al, 2007). Dependendo do local de hidrólise, as enzimas são classificadas como fosfolipase A, B, C, ou D, representado pela Figura 1 (KOHLER et al, 2006, SIMKHADA et al, 2009, JIANG et al, 2011 (YANG et al, 2008;DIJKSTRA, 2010).…”

Section: O Desenvolvimento Regional Das Pequenas E Médias Propriedadeunclassified

Degomagem Enzimática De Óleo De Mamona Produzido No Vale Do Rio Pardo

Amaral¹,

Silva²,

Silva³

et al. 2014

RJP

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RESUMOEste trabalho foi desenvolvido com o objetivo de realizar a otimização em escala semiindustrial de produção de óleo de mamona, que foi o objetivo de um projeto conjunto da Universidade de Santa Cruz do Sul (UNISC), Indústria Mercur e Cooperativa Mista de Fumicultores do Brasil (COOPERFUMOS). Foram analisados dois lotes de óleo de mamona, sendo um extraído pela UNISC, e outro fornecido pela COOPERFUMOS. Foram feitas analises da composição físico-química de óleo de mamona produzido na região do Vale do Rio Pardo para fins de aproveitamento do óleo para utilização em indústria. O óleo bruto passou por processo de degomagem enzimática. A extração feita pela UNISC teve uma redução acima de 80% de fosfolipídios, mostrando um resultado satisfatório, bem como apresentaram valores compatíveis com as exigências para a utilização na fabricação da borracha. Já o óleo da COOPERFUMOS teve uma redução de aproximadamente 50% dos fosfolipídios iniciais. Entende-se que o óleo da COOPERFUMOS por ser verde pode ter mascarado o resultado da determinação de fosfolipídios que é por turbidimetria. Com base nestes resultados foi possível constatar que a degomagem enzimática pode ser utilizada para a purificação do óleo de mamona.Palavras-chave: Óleo de Mamona. Degomagem Enzimática. Purificação. ABSTRACTThis work was developed in order to perform the optimization in semi-industrial scale production of castor oil, which was the objective of a project at the University of Santa Cruz do Sul (UNISC), Mercur and Cooperativa Mista de Fumicultores do Brasil (COOPERFUMOS) We analyzed two batches of oil being extracted by UNISC one and the other provided by Cooperfumos do Brasil SA. Analyses were made of the physical and chemical composition of castor oil produced in the Vale do Rio Pardo for the purpose of using the oil for use in industry. The crude oil has gone through the process of enzymatic degumming. The extraction was done by UNISC a reduction of over 80% phospholipids, showing a satisfactory result in their reduction and showed values compatible with the demands for use in the production of rubber. The COOPERFUMOS oil had a reduction of approximately 50% of its initial phospholipids, due to this, we note that it was green oil containing more impurities and require a further purification step.

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“…In the enzymatic oil-degumming process, phospholipases of type A and type C are commonly used (Jiang et al 2011). Phospholipase A (PLA) can cause the production of lysophospholipids, which would be removed with water during the separation stage of the degumming process (Manjula et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Optimization of the degumming process for camellia oil by the use of phospholipase C in pilot-scale system

et al. 2014

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In present study, phospholipase C (PLC) was applied in camellia oil degumming and the response surface method (RSM) was used to determine the optimum degumming conditions (reaction time, reaction temperature and enzyme dosage) for this enzyme. The optimum conditions for the minimum residual phosphorus content (15.14 mg/kg) and maximum yield of camellia oil (98.2 %) were obtained at reaction temperature 53ºC, reaction time 2.2 h, PLC dosage 400 mg/kg and pH 5.4. The application of phospholipase A (PLA) -assisted degumming process could further reduce the residual phosphorus content of camellia oil (6.84 mg/kg) to make the oil suitable for physical refining while maintaining the maximal oil yield (98.2 %). These results indicate that PLC degumming process in combination with PLA treatment can be a commercially viable alternative for traditional degumming process. Study on the quality changes of degummed oils showed that the oxidative stability of camellia oil was slightly deceased after the enzymatic treatment, thus more attention should be paid to the oxidative stability in the further application.

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Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18

Cited by 41 publications

References 27 publications

Streptomyces violaceoruber Phospholipase A2: Expression in Pichia pastoris, Properties, and Application in Oil Degumming

Streptomyces violaceoruber Phospholipase A2: Expression in Pichia pastoris, Properties, and Application in Oil Degumming

Degomagem Enzimática De Óleo De Mamona Produzido No Vale Do Rio Pardo

Optimization of the degumming process for camellia oil by the use of phospholipase C in pilot-scale system

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