Degumming of ramie fibers by alkalophilic bacteria and their polysaccharide-degrading enzymes

Zheng, Lijuan; Du, Yong; Zhang, Jiayao

doi:10.1016/s0960-8524(00)00154-1

Cited by 98 publications

(77 citation statements)

References 7 publications

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“…The results were close to the effects of applying that bacterial strain for the degumming of kenaf (Chiliveri et al, 2016;Liu et al, 2012). Compared with other degumming bacterial strains (Basu et al, 2009;Zheng et al, 2001), B. cereus has a shorter degumming cycle (10 h) and ideal gum removal capabilities (residual gum rate is less than 6 %) and produces fiber of good quality (breaking strength is more than 850 cN dtex −1…”

Section: Discussionsupporting

confidence: 66%

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Cheng

Wang

Feng

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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ABSTRACT:Biological degumming is an eco-friendly, efficient, high-quality and low-cost method that has become the leading bast fiber degumming technology. However, bacterial strains with short degumming cycles, high gum removal rates and small fiber damage are few. To screen high quality microbial resources with bast-fiber biological degumming function, soil samples were collected from a continuously cultivated banana plantation and then used to be enriched by ramie and kenaf materials in turn. A selective pectin-degrading medium was used to screen for excellent bacteria. A dominant bacterial strain was identified by phenotypic and genotypic characteristics, and its biological degumming effects on ramie and kenaf were verified by a comprehensive evaluation system. Results showed that seven bacterial strains secreting pectinase were obtained and the largest hydrolysis circle with a diameter ratio H/C of 2.4 was produced by the bacterial strain hn1-1, which was preliminarily identified as the Bacillus cereus by colony morphological characteristics and 16S rDNA sequence (GenBank accession number: KX013542) cluster analysis. The fiber production of ramie and kenaf degummed by B. cereus hn1-1 for 10 h were 72 % and 76 %, the residual gum rates were 4 % and 5 %, respectively. These values satisfied the textile industry requirement of < 6 % residual gum rate. Therefore, an effective biological degumming bacterium, B. cereus, was identified using a pectin-hydrolysis selective medium through a simple, economical, and time-saving method. Furthermore, the biological degumming technology by B. cereus for ramie and kenaf had a short cycle, ideal removal gum rate, and high-quality and productive fiber output.

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Section: Discussionsupporting

confidence: 66%

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Cheng

Wang

Feng

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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“…Pectinolytic activity played an active role in degumming of ramie bast fibers (Brühlmann et al, 1994;Brühlmann et al, 2000;Zheng et al, 2001). Xylanase also played an important role in the degradation of residual gum (Zheng et al, 2001), and, we found strain R10, R12 and R13 did not secret xylanase, however, they had better degomming function. Therefore, pectinase was the key enzyme of ramie degumming, and the xylanase and mannanase were just assistants.…”

mentioning

confidence: 54%

“…Cao et al selected four strains designated as NT-2, NT-6, NT-33 and NT-82, which all could produce pectinase (Cao et al, 1992). Pectinolytic activity played an active role in degumming of ramie bast fibers (Brühlmann et al, 1994;Brühlmann et al, 2000;Zheng et al, 2001). Xylanase also played an important role in the degradation of residual gum (Zheng et al, 2001), and, we found strain R10, R12 and R13 did not secret xylanase, however, they had better degomming function.…”

mentioning

confidence: 62%

“…(He and Feng, 1997), Actinomycete sp. (Brühlmann et al, 1994), Erwinia carotovorum (Peng et al, 1995), Amycolata sp., Streptomyces lividans (Brühlmann et al, 2000), Bacillus subtilis (Sun et al, 1979), Bacillus pumilus (Basu et al, 2009), some other alkalophilic Bacillus (Cao et al, 1992;Zheng et al, 2001), and Aspergillus sp. (Deshpande and Gurucharanam, 1985).…”

Section: -----------------------------------------------Cfu ---------mentioning

confidence: 99%

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Diversity and characterization of ramie-degumming strains

Duan

Liu

Feng

et al. 2012

Sci. agric. (Piracicaba, Braz.)

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Ramie (Boehmeria nivea and Boehmeria tenacissima) is a widely used fiber crop.Traditional water retting or chemical boiling method performed in order to extract ramie fiber seriously pollute the environment and severely damage the fiber, so biological method is the general trend of the fiber-extracting industry. Some strains (687), involving 26 genera and 43 species, were collected from the three samples, which produce hydrolyzed circles in the selective culture medium in order to detect the degumming effect and to compare the enzyme activity.Among these strains, 13 of them did not produce cellulase and had a ramie decreasing weight rate above 25 %, which were regarded as efficient ramie-degumming strains named from R1 Rhizobium sp. and Pseudomonas sp. were firstly reported to be used in ramie-degumming. At the same time, the pectinase was the key enzyme in the ramie-degumming process.

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“…Bacteria secreting alkaline pectinase were first used in the retting process of Mitsumata bast 52 . Alkaliphilic bacterial strain NT-33 can also degum ramie fibers 53 and the retting process of a type of Japanese paper has been improved considerably by the use of alkaline pecticlyase (optimal pH of 9.5) from Bacillus sp. strain GIR 277; this use leads to the production of stronger, better quality paper.…”

Section: Xylanasementioning

confidence: 99%

Industrial Important Enzymes from Alkaliphiles – An overview

Chinnathambi¹

2015

Biosci., Biotech. Res. Asia

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Alkaliphiles are an interesting group of extremophilic organisms that thrive at pH of 9.0 and above and which are represented by prokaryotes, eukaryotes, and archaea. It is clear that a variety of taxa are represented among the alkaliphiles, some of which are new. Alkaliphiles can be isolated from normal environments such as garden soil, although the number isolated increases dramatically in highly alkaline environments. Many alkaliphile products, notably enzymes, have found widespread practical applications, primarily in the detergent and laundry industries; other potentially important products isolated from alkaliphiles include antibiotics and carotenoids. Alkaliphiles, and their products, are also potentially important for use in the degradation of xenobiotics, and they play a major role in the biogeocycling of key inorganic compounds. This review provides an insight into the huge diversity of alkaliphilic bacteria, the varied products obtained from them, and the need for further investigations on this interesting group of bacteria.

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Degumming of ramie fibers by alkalophilic bacteria and their polysaccharide-degrading enzymes

Cited by 98 publications

References 7 publications

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Diversity and characterization of ramie-degumming strains

Industrial Important Enzymes from Alkaliphiles – An overview

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