2016
DOI: 10.1007/s00253-016-8023-5
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Degrons of yeast and mammalian ornithine decarboxylase enzymes make potent combination for regulated targeted protein degradation

Abstract: Elevation of polyamine levels in eukaryotes leads to rapid degradation of ornithine decarboxylase (ODC), the first enzyme of polyamine biosynthesis pathway. ODC in yeast (yODC) has two domains, the Nα/β domain consisting of α/β barrel domain (α/β) preceded by an overhang of 50 residues at its N-terminus (N50) and β sheet domain at its C-terminus. Two degradation determinant signals or degrons in yODC sequence, namely the N50 and the antizyme-binding element (AzBE) housed in the α/β domain, are responsible for … Show more

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Cited by 4 publications
(4 citation statements)
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“…To further characterize the sensor constructs, we studied four variants of the sensor: one where GFP is expressed followed solely by the linker sequence (no insert); a second variant in which the well‐characterized ornithine decarboxylase degron is appended downstream of the linker (ODC d2 ) (Joshi & Prabha, 2017 ; Takeuchi et al, 2008 ); and additionally, the previously studied constructs with the c‐Src and Lck kinase domains (Src KD , Lck KD ). ODC d2 is posttranslationally degraded by the ubiquitin proteasome system and should behave similarly to a strong HSP90‐client when the chaperone is inhibited.…”
Section: Resultsmentioning
confidence: 99%
“…To further characterize the sensor constructs, we studied four variants of the sensor: one where GFP is expressed followed solely by the linker sequence (no insert); a second variant in which the well‐characterized ornithine decarboxylase degron is appended downstream of the linker (ODC d2 ) (Joshi & Prabha, 2017 ; Takeuchi et al, 2008 ); and additionally, the previously studied constructs with the c‐Src and Lck kinase domains (Src KD , Lck KD ). ODC d2 is posttranslationally degraded by the ubiquitin proteasome system and should behave similarly to a strong HSP90‐client when the chaperone is inhibited.…”
Section: Resultsmentioning
confidence: 99%
“…To further characterize the sensor constructs, we studied four variants of the sensor: one where GFP is expressed followed solely by the linker sequence (no insert); a second variant in which the well-characterized ornithine decarboxylase degron is appended downstream of the linker (ODC d2 ) 57,58 ; and additionally, the previously studied constructs with the c-Src and Lck kinase domains (Src KD , Lck KD ). ODC d2 is post-translationally degraded by the ubiquitin proteasome system and should behave similarly to a strong HSP90-client when the chaperone is inhibited.…”
Section: The Calibrated Sensor Reports On Proteasomal Degradation Of ...mentioning
confidence: 99%
“…One destabilized GFP named yeGFP-mODC contained a mouse Ornithine decarboxylase (mODC) PEST sequence [58] fused to the C-terminal of the yeast-enhanced GFP (yeGFP). This targets the GFP to the proteasome in a ubiquitin-independent manner [58] and destabilizes the protein in yeast [59]. The other destabilized GFP used in the study has the PEST sequence from the yeast protein Cyclin-2 fused to the c-terminal of yeGFP [60,61].…”
Section: Estimating Single Cell Proteasomal Decay Ratesmentioning
confidence: 99%