A fluorescence‐based sensor for calibrated measurement of protein kinase stability in live cells
Joseph W. Paul,
Serena Muratcioğlu,
John Kuriyan
Abstract:Oncogenic mutations can destabilize signaling proteins, resulting in increased or unregulated activity. Thus, there is considerable interest in mapping the relationship between mutations and the stability of signaling proteins, to better understand the consequences of oncogenic mutations and potentially inform the development of new therapeutics. Here, we develop a tool to study protein‐kinase stability in live mammalian cells and the effects of the HSP90 chaperone system on the stability of these kinases. We … Show more
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