Degradation of Synthetic Dyes by Laccases – A Mini-Review

Legerská, Barbora; Chmelová, Daniela; Ondrejovič, Miroslav

doi:10.1515/nbec-2016-0010

Cited by 74 publications

(59 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…SAC01, and also Alcántara et al (2017) for yeast species. Previous studies have indicated that the toxicity of azo dyes strongly depends on chemical structure, functional groups and the number of azo bonds (Costa et al 2012;Legerská et al 2016;Da Costa et al 2017). This was also confirmed in this study showing the relatively higher toxicity of α-naphtol dye Acid Violet 7 comparing to β-naphtol dye Orange G. Even more importantly, laccase treatment resulted in degradation products with no toxicity on tested microorganisms (Table 3).…”

Section: Toxicity Testsmentioning

confidence: 99%

“…Azonaphthalene dyes have naphthalene ring with delocalized conjugated bonds of carbon atoms stabilise total structure of azo dye (Zhu et al 2012). Moreover, functional groups present in their structure are responsible for various physical properties such as solubility, lipophilicity or absorption (Legerská et al 2016;Da Costa et al 2017). Approximately 2 -50 % of synthetic dyes used in industrial dyeing operations have been discharged into wastewater (Sarkar et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Biological elimination of synthetic azo dyes is environmentally friendly and relatively inexpensive. Moreover, the biological methods do not form any toxic degradation products (Sathe et al 2015;Legerská et al 2016, Chmelová and. Useful enzymes for this purpose seem to be azoreductases (EC 1.7.1.6), laccases (EC 1.10.3.2) and peroxidases (1.11.1.x) (Viswanath et al 2014;Singh et al 2015).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Azonaphthalene dyes decolorization and detoxification by laccase from Trametes versicolor

Legerská¹,

Chmelová²,

Ondrejovič³

2018

Nova Biotechnologica Et Chimica

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to investigate the dye decolorization ability of laccase from Trametes versicolor. Five azonaphthalene dyes (Acid Violet 7, Acid Red 1, Allura Red AC, Orange G and Sunset Yellow FCF) were used to evaluate dye decolorization. Laccase from T. versicolor is capable of decolorizing dyes, namely Acid Violet 7 (53.7±2.3 %) and Orange G (46.0±2.2 %). The less effective ability of laccase was observed at the decolorization of other selected dyes (6.9 -18.6 %). The presence of redox mediator (1-hydroxybenzotriazole) increased decolorization percentage for all tested dyes (≥ 90.5 %). Toxic effect of azo dyes and their degradation products after laccase treatment was observed on the growth of selected bacteria (Micrococcus luteus, Bacillus subtilis, Pseudomonas syringae and Escherichia coli), yeasts (Candida parapsilosis and Saccharomyces cerevisiae) and algae (Chlorella vulgaris and Microcystis aeruginosa). It was confirmed that degradation products showed lower inhibition effect compared to initial dyes. These findings suggest that laccase from T. versicolor are able to decolorize and detoxify selected azonaphthalene dyes.

show abstract

Section: Toxicity Testsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Azonaphthalene dyes decolorization and detoxification by laccase from Trametes versicolor

Legerská¹,

Chmelová²,

Ondrejovič³

2018

Nova Biotechnologica Et Chimica

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among the various fungi, white rot fungi are the well-known and potent producers of laccase. In recent years laccase have attracted commercial significance due to their extensive use in food (Minussi et al 2002;Rodrìguez and Toca Herrera 2006), textile (Abadulla et al 2000), pulp and paper industry, waste water treatment (Casa et al 2003), bioremediation (Duran and Esposito 2000), nano-biotechnology, agrobiotechnology and pharmaceutical industries (Rodríguez-Delgado et al 2015;Legerská et al 2016). Laccase can be produced by batch, fed-batch or continuous cultivation.…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization for enhanced production of extracellular laccase from Aspergillus sp. HB_RZ4 isolated from bark scrapping

Bhamare

Jadhav

Sayyed

2018

Environmental Sustainability

View full text Add to dashboard Cite

Laccase enzyme has gained tremendous demand in various industries, waste water treatment, bioremediation and nanobiotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential laccase producing cultures and statistical optimization. Use of Plackett-Burman design (PBD) that evaluates various medium components and having two level factorial design help to determine the factor and its level to increase the yield of product. Present paper reports the screening of laccase producting fungus identified as Aspergillus sp. by 18S rDNA of internal transcribed spacer regions and the large subunit. Aspergillus sp. HB_RZ4 isolated from tree bark scrapping showed enhanced production of laccase in minimal medium by applying two-stage statistical approach, as PBD and Response Surface Methodology using central composite design (CCD). In the first stage of optimization, out of 8 variables of minimal medium, glucose, yeast extract, CuSO 4 and temperature were found as significant components that influenced laccase production. Aspergillus sp. HB_RZ4 produced highest amount (7.27 Uml −1 ) of extracellular laccase. Further optimization using CCD revealed that 34 °C temperature, 2.1 gL −1 of glucose (carbon source), 2.5 gL −1 of yeast extract (nitrogen source) and 0.0025 gLCuSO 4 (inducer) were the optimum values that yielded maximum laccase. The optimization by PBD statistical design studies revealed optimum laccase yield with conditions being; pH, 6.0; temperature, 34 °C; incubation, 9 days; glucose, 2.1 gL −1 ; yeast extract, 2.5 gL −1 and CuSO 4 ., 0.0025 gL −1 .

show abstract

“…Therefore, these enzymes are potentially used for different industrial applications including agro-food, chemical or pharmaceutical industries (Rodríguez-Delgado et al 2015;Legerská et al 2016). The main problem of laccase applications for the commercial purposes is the high costs of their production.…”

Section: Introductionmentioning

confidence: 99%

The optimization of propagation medium for the increase of laccase production by the white-rot fungus Pleurotus ostreatus

Hazuchová¹,

Chmelová²,

Ondrejovič³

2017

Nova Biotechnologica Et Chimica

Self Cite

View full text Add to dashboard Cite

The lignocellulolytic enzymes are routinely produced by submerged fermentation using lignocellulosic material, but for more effective production, it would be suitable to precede the production phase on the lignocellulose by propagation phase in the nutrition medium suitable for growth of the fungi. Therefore, the aim of this study was to increase the laccase production by the white-rot fungus Pleurotus ostreatus by two-step cultivation strategy. In the first step, propagation medium was optimized for the maximal biomass growth, the second step included the laccase production by produced fungal biomass in media with the selected lignocellulosic material (pine sawdust, alfalfa steam and corn straw). From our experiments, parameters such as glucose concentration, yeast extract concentration and pH of propagation medium were selected as key factors affecting growth of P. ostreatus. The optimal conditions of propagation medium for maximal fungal growth determined by response surface methodology were: glucose concentration 102.68 g/L, yeast extract concentration 43.65 g/L and pH of propagation medium 7.24. These values were experimentally verified and used statistical model of biomass production prediction was appropriate adjusted. Thus prepared fungal biomass produced in the media with lignocellulose approximately 9-16 times higher concentrations of the laccase in 3 times shorter time than the fungal biomass without propagation phase in optimized propagation medium.

show abstract

Degradation of Synthetic Dyes by Laccases – A Mini-Review

Cited by 74 publications

References 42 publications

Azonaphthalene dyes decolorization and detoxification by laccase from Trametes versicolor

Azonaphthalene dyes decolorization and detoxification by laccase from Trametes versicolor

Statistical optimization for enhanced production of extracellular laccase from Aspergillus sp. HB_RZ4 isolated from bark scrapping

The optimization of propagation medium for the increase of laccase production by the white-rot fungus Pleurotus ostreatus

Contact Info

Product

Resources

About