Degradation of sulfonated azo dyes by the purified lignin peroxidase from Brevibacillus laterosporus MTCC 2298

Gomare, Sushama S.; Jadhav, Jyoti P.; Govindwar, Sanjay P.

doi:10.1007/s12257-008-0008-5

Cited by 29 publications

(19 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yield of sugars from rice hulls by acid treatment under optimized conditions was 252 mg/g, which is about 58% of a theological yield based on the content of crude fiber in rice hulls. High content of lignin in cellulosic materials has a negative effect on enzymatic saccharification by cellulase [20,23]. Removal rate of lignin from acidic pretreated rice hulls by 1.0% sodium hydroxide increased with reaction temperature and time.…”

Section: Resultsmentioning

confidence: 99%

Pretreatment and saccharification of rice hulls for the production of fermentable sugars

Wei

Gao

Jin

et al. 2009

Biotechnol Bioproc E

View full text Add to dashboard Cite

Hydrolytic conditions of rice hulls by acid and alkaline treatments before enzymatic saccharification were optimized in this study. Based on the results of single-factor experiments and an orthogonal array experiment, reaction time was found to be the most important factor for the acidic hydrolysis of rice hulls. Maximum yield of sugars from 1 g of rice hulls by acidic treatment under optimized conditions was 213.6 mg. The yield of lignin removal from acidic pretreated rice hulls by alkaline treatment increased with increase in reaction temperature and time. The amount of sugars obtained from 1 g of pretreated rice hulls by enzymatic saccharification was 307.7 mg, and the conversion rate of sugars from crude fibers in pretreated rice hulls was about 72%. Instrumental analyses with FTIR and SEM indicated that lignin in rice hulls was partially removed by alkaline treatment, and the structure of rice hulls became deformed and more fibers were exposed to cellulases after acidic treatment. © KSBB hÉóïçêÇëW=êáÅÉ=ÜìääëI=éêÉíêÉ~íãÉåíI=ÜóÇêçäóëáëI=ë~ÅÅÜ~êáÑáÅ~íáçåI=ÅÉääìä~ëÉI=ÑÉêãÉåí~ÄäÉ=ëìÖ~êë= = = = =

show abstract

Section: Resultsmentioning

confidence: 99%

Pretreatment and saccharification of rice hulls for the production of fermentable sugars

Wei

Gao

Jin

et al. 2009

Biotechnol Bioproc E

View full text Add to dashboard Cite

show abstract

“…Lignin peroxidase catalyzes the oxidative breakdown of azo dye Disperse orange 3, resulting in the formation of nitrobenzene as a major product (Zhao et al 2006). The role of purified lignin peroxidase in the degradation of azo dyes like Methyl orange and Methyl red has also been reported (Gomare et al 2008;Dawkar et al 2009;Ghodake et al 2009). …”

Section: Introductionmentioning

confidence: 89%

Purification and characterization of a bacterial peroxidase from the isolated strain Pseudomonas sp. SUK1 and its application for textile dye decolorization

et al. 2010

Self Cite

View full text Add to dashboard Cite

The intracellular bacterial peroxidase from Pseudomonas sp. SUK1 was purified by anion exchange and molecular sieve chromatography. The molecular weight of the purified peroxidase was estimated to be 86 kDa by SDS-PAGE analysis. The UV-visible absorption spectra revealed that the purified peroxidase was a heme-containing protein. The purified enzyme exerted its optimal activity with n-propanol and also oxidized various lignin-related phenols, whereas no activity was observed with p-cresol and veratrole. Optimum pH and temperature for the purified peroxidase were 3.0 and 40°C, respectively. The purified enzyme proficiently decolorized various textile dyes. Methyl orange was used as a model azo dye to understand the mechanism of action of peroxidase in dye degradation. Further, dye degradation was confirmed by analytical techniques like FTIR, HPLC and GC-MS.

show abstract

“…These dyes or their degradation products, when released into water even at very low concentrations can be toxic and sometimes carcinogenic, mutagenic, or teratogenic to various organisms, including humans (Novotny et al 2006; Hai et al 2007). Large amounts of the dyestuff, which are usually unbound dye materials, are directly lost to the wastewater, during textile processing (Gomare et al 2008). In the present scenario, the release of colored wastewater to the ecosystem is major source of eutrophication and perturbations in aquatic life.…”

Section: Introductionmentioning

confidence: 99%

Biotransformation studies of textile dye Remazol Orange 3R

et al. 2012

Self Cite

View full text Add to dashboard Cite

In the present study, biotransformation of Remazol Orange 3R (RO3R) was studied using well-known bacterial isolate Pseudomonas aeruginosa strain BCH. The dye was decolorized up to 98 % within 15 min. The induction in the level of various oxidoreductive enzymes viz. laccase, tyrosinase, veratryl alcohol oxidase and DCIP reductase were observed in the cells obtained after decolorization of RO3R, which supports their role in decolorization. The metabolites of RO3R obtained after biodegradation were identified and characterized by various analytical techniques viz, HPLC, FTIR, and GC–MS. The RO3R was transformed to the N-(7 amino 8 hydroxy-napthalen-2yl) actamide (m/z, 198), Acetamide (m/z, 59) and Napthalen-1-ol (m/z, 144).

show abstract

Degradation of sulfonated azo dyes by the purified lignin peroxidase from Brevibacillus laterosporus MTCC 2298

Cited by 29 publications

References 30 publications

Pretreatment and saccharification of rice hulls for the production of fermentable sugars

Pretreatment and saccharification of rice hulls for the production of fermentable sugars

Purification and characterization of a bacterial peroxidase from the isolated strain Pseudomonas sp. SUK1 and its application for textile dye decolorization

Biotransformation studies of textile dye Remazol Orange 3R

Contact Info

Product

Resources

About