Degradation of Phytate Pentamagnesium Salt by Bacillus sp. T4 Phytase as a Potential Eco-friendly Feed Additive

Abstract

A bacterial isolate derived from soil samples near a cattle farm was found to display extracellular phytase activity. Based on 16S rRNA sequence analysis, the strain was named Bacillus sp. T4. The optimum temperature for the phytase activity toward magnesium phytate (Mg-InsP6) was 40°C without 5 mM Ca2+ and 50°C with 5 mM Ca2+. T4 phytase had a characteristic bi-hump two pH optima of 6.0 to 6.5 and 7.4 for Mg-InsP6. The enzyme showed higher specificity for Mg-InsP6 than sodium phytate (Na-InsP6). Its activity was fairly inhibited by EDTA, Cu2+, Mn2+, Co2+, Ba2+ and Zn2+. T4 phytase may have great potential for use as an eco-friendly feed additive to enhance the nutritive quality of phytate and reduce phosphorus pollution.

A novel extracellular low‐temperature active phytase from Bacillus aryabhattai RS1 with potential application in plant growth

“…KHU-10 30 and Bacillus sp. T4, 33 whereas several phytases from the members of the same genus had greater temperature optima, such as, 558C for B. subtilis 12 and B. nealsonii, 15 658C for B. licheniformis 31 and 708C for B. laevolacticus. 13 Unlike other Bacillus phytases, the RS1 phytase exhibited marked (>40%) activity at lower temperature of even 108C.…”

“…T4 phytase was stable up to 408C, but at 508C it retained 10 and 70% of the initial activity in absence and presence of Ca 21 , respectively. 33 Most Bacillus phytases are reported to have a high specificity for sodium phytate 12,15 and so do the B. aryabhattai RS1 phytase. The enzyme activity was enhanced by Mg 21 and Ca 21 and inhibited by Zn 21 , Cu 21 , Mn 21 , Co 21 , Fe 21 , and EDTA, as reported earlier for phytase from B. nealsonii ZJ070.…”

“…As observed in the present work, Bacillus sp. T4 phytase was stable up to 40°C, but at 50°C it retained 10 and 70% of the initial activity in absence and presence of Ca 2+ , respectively . Most Bacillus phytases are reported to have a high specificity for sodium phytate and so do the B. aryabhattai RS1 phytase.…”

Unravelling the Role of Rhizosphere Microbiome and Root Traits in Organic Phosphorus Mobilization for Sustainable Phosphorus Fertilization. A Review

“…The phytase from Fusarium verticillioides showed an optimal temperature of 50 • C and stability up to 60 • C [104]. The optimal temperature for phytase activity towards magnesium phytate (Mg-IHP) has been reported to reach 40 • C without and 50 • C with 5 mM Ca 2+ [111] Most plant phytases have an optimal temperature of 45-60 • C, as reported by Johnson et al [112]. The lowest temperature has been reported to be 10 • C [110].…”

Efficient bio-deodorization of thioanisole by a novel bacterium Brevibacillus borstelensis GIGAN1 immobilized onto different parking materials in twin biotrickling filter

“…Genomic DNA was extracted using genomic DNA isolation kit (Sangon Biotech Co., Ltd., Shanghai), and used as the template for PCR amplification. Universal bacterial primers: 27 F (5 0 -AGAGTTTGATCCTGGCTCAG -3 0 ) and 1492 R (5 0 -GGTTACCTTGTTACGACTT -3 0 ) (Park et al, 2012) was designed to amplify the 16 sRNA gene by PCR. Experimental details are listed in the SI.…”