A novel extracellular low‐temperature active phytase from Bacillus aryabhattai RS1 with potential application in plant growth

Abstract

Bacillus aryabhattai RS1 isolated from rhizosphere produced an extracellular, low temperature active phytase. The cultural conditions for enzyme production were optimized to obtain 35 U mL of activity. Purified phytase had specific activity and molecular weight of 72.97 U mg and ∼40 kDa, respectively. The enzyme was optimally active at pH 6.5 and 40°C and was highly specific to phytate. It exhibited higher catalytic activity at low temperature, retaining over 40% activity at 10°C. Phytase was more thermostable in presence of Ca ion and retained 100% residual activity on preincubation at 20-50°C for 30 min. Partial phytase encoding gene, phy (816 bp) was cloned and sequenced. The encoded amino acid sequence (272 aa) contained two conserved motifs, DA[A/T/E]DDPA[I/L/V]W and NN[V/I]D[I/L/V]R[Y/D/Q] of β-propellar phytase and had lower sequence homology with other Bacillus phytases, indicating its novelty. Phytase and the bacterial inoculum were effective in improving germination and growth of chickpea seedlings under phosphate limiting condition. Moreover, the potential applications of the enzyme with relatively high activity at lower temperatures (20-30°C) could also be extended to aquaculture and food processing. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:633-641, 2017.

Phylogenetic diversity and biotechnological potentials of marine bacteria from continental slope of eastern Arabian Sea

“…B. pumilus (MSB6) showed highest amylase activity with maximum zone of clearance. B. aryabhattai (MSB1) showed efficient phytate degrading activity with a maximum zone of clearance around the colony, supporting the observations made by Pal Roy et al [21]. Among Gram-negative bacterial isolates from hypersaline environment, most of the hydrolytic enzyme producers were belonged to the genus Halomonas [22].…”

Ornamental cabbage (Brassica oleracea var. acephala) responses to phytase enzyme purified from Lactobacillus coryniformis application

“…Application of Bacillus aryabhattai RS1 as seed and soil inoculants has been reported to improve the growth of chickpea seedlings with a significant increase in total plant biomass in the absence of additional inorganic phosphate in the soil. 29 In the present study, it was concluded that the inoculation time is important in the inoculation of the phytase enzyme, which is applied after being purified from the microorganism, and that the inoculation time should be at least 30 min.…”

“…In this study, it is estimated that the reason for the increase in the width of the plant crown is that the plant nutrition, especially the nutrient dissolution and uptake of phosphorus, is facilitated. In previous studies, [26][27][28][29] it has been reported that microorganisms that play a role in phosphorus solubility positively affect plant growth and development. The main use of phytase supplementation in soil is to reduce the frequent application of chemical phosphate fertilizers to the soil.…”

“…28 It has been reported that applications of phytase and the bacterial inoculum had positive effects on germination of chickpea seed and growth of chickpea seedlings under phosphate-limiting condition. 29 F I G U R E 1 Beginning of the emergence of ornamental cabbage seeds and the period of cotyledonous leaves (A); two to three leaf period of ornamental cabbage seedlings (B); their period when the seedlings had four to five true leaves (C); and the period in which all parameters were measured and measurement of diameter center color parameter (D).…”

Harnessing rhizobacteria to fulfil inter-linked nutrient dependency on soil and alleviate stresses in plants

“…Besides inorganic phosphate, phytates are the prevalent storage form of organic P in plants which can be sequentially hydrolysed by microbial phytases to enhance P availability in soil and in the plants. For instance, an increased chickpeas seed germination, plant biomass and P content were observed after inoculation with phytase‐producing Bacillus aryabhattai RS1, under limiting P conditions (Roy et al, 2017). The role of phytases in a plant's internal metabolism has also been suggested besides in hydrolysing soil phytate.…”